Establishment of a Modified and Standardized Ferric Chloride-Induced Rat Carotid Artery Thrombosis Model

doi:10.1021/acsomega.1c07316

科研成果详情

题名	Establishment of a Modified and Standardized Ferric Chloride-Induced Rat Carotid Artery Thrombosis Model
作者	Lin, Xiao1,2; Zhao, Peiqi 1,2; Lin, Zhongxiao 1,2; Chen, Jiayu1,2; Bingwa, Lebohang Anesu 1; Siaw-Debrah, Felix 4; Zhang, Peng1,2; Jin, Kunlin 3; Yang, Su 1,2; Zhuge, Qichuan1,2
发表日期	2022-03-15
发表期刊	ACS omega 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	PLASMINOGEN-ACTIVATOR ; STROKE ; ANTIPLATELET ; ISCHEMIA ; INJURY
摘要	Background:Ferric chloride is widely utilized in inducing thrombosis in small vessels of experimental animals. However, the lack of its application in large blood vessels of experimental animals and inconsistent concentration has limited its application. Therefore, we systematically tested the most suitable concentration and reliable induction time in the experiment of using ferric chloride to induce rat carotid artery thrombosis. Methods:In this study, we selected the common carotid artery of 59 Sprague-Dawley rats as the target vessel. The exploration process was divided into three stages. First, to determine the optimum induction concentration, we compared the effects of 30-60% ferric chloride on thrombus formation within 24 h. Second, to confirm the handling time, we tested different induction times from 3 min to 10 min. Lastly, we used the thrombolytic drug rt-PA to detect whether the formed thrombus can be lysed. Doppler blood flow imaging and H-E staining were employed to estimate the blood flow and thrombus. The ATP levels in the brain were measured using a bioluminescence ATP assay kit. Results:We found that the application of 50% ferric chloride for 10 min was enough to successfully induce thrombosis in the rat carotid artery and without spontaneous thrombolysis after 24 h. It is better than other concentrations and will lead to the decline of the ATP content in the ischemic hemisphere. Conclusions:Our results indicate that the rat carotid artery thrombosis model induced by 50% ferric chloride for 10 min is stable and reliable.
资助项目	National Natural Science Foundation of China[81771262];
出版者	AMER CHEMICAL SOC
出版地	WASHINGTON
ISSN	2470-1343
卷号	7 期号:10 页码:8919-8927.
DOI	10.1021/acsomega.1c07316
页数	9
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
WOS记录号	WOS:000783601100069
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	35309441
PMC记录号	PMC8928333
SCOPUSEID	2-s2.0-85126749707
通讯作者地址	[Yang, Su]Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Zhuge, Qichuan]Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Chemistry (all);Chemical Engineering (all)
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33550
专题	附属第一医院_神经外科第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Yang, Su; Zhuge, Qichuan
作者单位	1.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Pharmacology and Neuroscience,University of North Texas,Health Science Center,Fort Worth,76107,United States; 4.Department of Neurosurgery,Korlebu Teaching Hospital,Korlebu,00233,Ghana
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Xiao,Zhao, Peiqi,Lin, Zhongxiao,et al. Establishment of a Modified and Standardized Ferric Chloride-Induced Rat Carotid Artery Thrombosis Model[J]. ACS omega,2022,7(10):8919-8927..
APA	Lin, Xiao., Zhao, Peiqi., Lin, Zhongxiao., Chen, Jiayu., Bingwa, Lebohang Anesu., ... & Zhuge, Qichuan. (2022). Establishment of a Modified and Standardized Ferric Chloride-Induced Rat Carotid Artery Thrombosis Model. ACS omega, 7(10), 8919-8927..
MLA	Lin, Xiao,et al."Establishment of a Modified and Standardized Ferric Chloride-Induced Rat Carotid Artery Thrombosis Model".ACS omega 7.10(2022):8919-8927..