题名 | FGF21 attenuates hypoxia-induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress |
作者 | |
发表日期 | 2018-09 |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | pulmonary arterial hypertension endoplasmic reticulum stress fibroblast growth factor 21 human pulmonary arterial endothelial cells |
其他关键词 | PULMONARY ARTERIAL-HYPERTENSION ; GROWTH-FACTOR 21 ; UNFOLDED PROTEIN RESPONSE ; SMOOTH-MUSCLE-CELLS ; ER-STRESS ; ENDOTHELIAL-CELLS ; NITRIC-OXIDE ; CROSS-TALK ; EXPRESSION ; PATHWAY |
摘要 | Vascular endothelial apoptosis and dysfunction have a crucial role in triggering pathological vascular remodeling of hypoxia-induced pulmonary arterial hypertension (PAH). Fibroblast growth factor (FGF)21, an endocrine regulator, has recently been reported to protect cardiac endothelial cells from damage and suppress inflammatory responses. In addition, FGF21 is reported to be involved in endoplasmic reticulum stress (ERS). Previous studies have suggested that ERS participates in the development of PAH, and attenuation of ERS could be an effective therapeutic strategy for the protection of pulmonary arteries. However, whether FGF21 has a protective function via suppression of ERS in pulmonary arterial endothelial cells in hypoxia remains unclear. The present study aimed to explore whether FGF21 could reduce the hypoxia-induced apoptosis of human pulmonary arterial endothelial cells (HPAECs) and prevent endothelial dysfunction via the inhibition of ERS. HPAECs were divided into six groups: Normoxia, hypoxia, hypoxia plus FGF21, hypoxia plus salubrinal (an ERS inhibitor), hypoxia plus tunicamycin (an ERS agonist), and hypoxia plus tunicamycin plus FGF21. The endoplasmic reticulum ultrastructure in HPAECs was assessed by transmission electron microscopy, and proliferation and apoptosis were examined by cell counting kit-8 and terminal deoxyribonucleotide transferase-mediated dUTP nick end-labelling assays, respectively. The expression levels of ERS-related proteins, including binding immunoglobulin protein (BiP), protein kinase R-like endoplasmic reticulum kinase (PERK), phosphorylated (p-) PERK, transcription factor C/EBP homologous protein (CHOP), B-cell lymphoma-2 (Bcl-2) and caspase-4 were detected by western blotting. Transwell migration chamber assays were performed, and the concentration of nitric oxide (NO)/endothelin-1 (ET-1) in the culture medium was determined to examine endothelial function. The results revealed that hypoxia increased the % of apoptotic cells and diminished the viability of HPAECs, accompanied by an upregulation of ERS-dependent apoptosis by increasing the expression of the proapoptotic caspase-4 and decreasing the antiapoptotic Bcl-2. Additionally, hypoxia upregulated the expression of representative proteins in the PERK branch of ERS, including BiP, p-PERK and CHOP, while it downregulated the expression of PERK. Furthermore, the secretion of NO/ET-1 and the migration rate of HPAECs were downregulated under conditions of hypoxia. FGF21 significantly attenuated the hypoxia-induced apoptosis and dysfunction of HPAECs through alleviating the aforementioned changes in ERS-dependent signaling pathways. In conclusion, ERS may be a crucial mechanism in the hypoxia-induced apoptosis and endothelial dysfunction of HPAECs. FGF21 may attenuate the hypoxia-induced apoptosis and dysfunction of HPAECs through alleviating ERS, via the PERK/CHOP signaling pathway and inhibition of caspase-4 expression. |
资助项目 | Project of Health Department of Zhejiang Province of China [2016DTA005]; Natural Science Foundation Grants of Zhejiang Province [Y17H010028]; Chinese National Natural Science Foundation Grants [81473406] |
出版者 | SPANDIDOS PUBL LTD |
出版地 | ATHENS |
ISSN | 1107-3756 |
EISSN | 1791-244X |
卷号 | 42期号:3页码:1684-1694 |
DOI | 10.3892/ijmm.2018.3705 |
页数 | 11 |
WOS类目 | Medicine, Research & Experimental |
WOS研究方向 | Research & Experimental Medicine |
WOS记录号 | WOS:000446875200047 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 29845288 |
SCOPUSEID | 2-s2.0-85049576789 |
通讯作者地址 | [Huang, Xiaoying]Division of Pulmonary Medicine,First Affiliated Hospital of Wenzhou Medical University,Key Laboratory of Heart and Lung,1 Nanbaixiang Street,Ouhai, Wenzhou, Zhejiang,325000,China |
Scopus学科分类 | Genetics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/3302 |
专题 | 附属第一医院 药学院(分析测试中心)_生物制药系_生物制药工程 |
通讯作者 | Huang, Xiaoying |
作者单位 | 1.Division of Pulmonary Medicine,First Affiliated Hospital of Wenzhou Medical University,Key Laboratory of Heart and Lung,1 Nanbaixiang Street,Ouhai, Wenzhou, Zhejiang,325000,China; 2.Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province,Wenzhou Medical University,Wenzhou, Zhejiang,325000,China; 3.Department of Respiratory Medicine,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an, Shanxi,710061,China |
第一作者单位 | 附属第一医院 |
通讯作者单位 | 附属第一医院 |
第一作者的第一单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Chen, Ali,Liu, Jingjing,Zhu, Jianfeng,et al. FGF21 attenuates hypoxia-induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2018,42(3):1684-1694. |
APA | Chen, Ali., Liu, Jingjing., Zhu, Jianfeng., Wang, Xuetao., Xu, Zhaona., ... & Huang, Xiaoying. (2018). FGF21 attenuates hypoxia-induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 42(3), 1684-1694. |
MLA | Chen, Ali,et al."FGF21 attenuates hypoxia-induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 42.3(2018):1684-1694. |
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