Rapid well-plate assays for motor and social behaviors in larval zebrafish

doi:10.1016/j.bbr.2020.112625

科研成果详情

题名	Rapid well-plate assays for motor and social behaviors in larval zebrafish
作者	Shen, Qiaosen 1,2,3; Truong, Lisa 2; Simonich, Michael T.2; Huang, Changjiang 3; Tanguay, Robyn L.2; Dong, Qiaoxiang1,3
发表日期	2020-08-05
发表期刊	BEHAVIOURAL BRAIN RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Behavioral paradigm Zebrafish Neurobehavioral Rapid throughput screening
其他关键词	ADULT ZEBRAFISH ; DEVELOPMENTAL EXPOSURE ; NICOTINE ; MODEL ; RESPONSES ; KETAMINE ; DEFICITS ; FEMALE ; PICROTOXIN ; FLUOXETINE
摘要	Behavior phenotypes are a powerful means of uncovering subtle xenobiotic chemical impacts on vertebrate nervous system development. Rodents manifest complex and informative behavior phenotypes but are generally not practical models in which to screen large numbers of chemicals. Zebrafish recapitulate much of the behavioral complexity of higher vertebrates, develop externally and are amenable to assay automation. Short duration automated assays can be leveraged to screen large numbers of chemicals or comprehensive dose-response for fewer chemicals. Here we describe a series of mostly automated assays including larval photomotor response, strobe light response, blue color avoidance, shoaling and mirror stimulus-response performed on the ZebraBox (ViewPoint Behavior Technologies) instrument platform. To explore the sensitivity and uniqueness of each assay endpoint, larval cohorts from 5 to 28 days post fertilization were acutely exposed to several chemicals broadly understood to impact different neuro-activities. We highlight the throughput advantages of using the same instrument platform for multiple assays and the ability of different assays to detect unique phenotypes among different chemicals.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21477089]; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [P42 ES016465]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	0166-4328
EISSN	1872-7549
卷号	391 页码:112625
DOI	10.1016/j.bbr.2020.112625
页数	10
WOS类目	Behavioral Sciences ; Neurosciences
WOS研究方向	Behavioral Sciences ; Neurosciences & Neurology
WOS记录号	WOS:000540163600001
收录类别	SSCI ; SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	32428631
PMC记录号	PMC7341899
Scopus记录号	2-s2.0-85085582843
通讯作者地址	[Tanguay, Robyn L.]Sinnhuber Aquatic Research Laboratory,Department of Environmental & Molecular Toxicology,Oregon State University,1007 Agriculture & Life Sciences Building,Corvallis,97331,United States
scopus学科分类	Behavioral Neuroscience
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2622
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院公共卫生与管理学院_健康与环境生态研究所公共卫生与管理学院_环境安全与健康风险评估研究院
通讯作者	Tanguay, Robyn L.
作者单位	1.The Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Wenzhou,325035,China; 2.Sinnhuber Aquatic Research Laboratory,Department of Environmental & Molecular Toxicology,Oregon State University,1007 Agriculture & Life Sciences Building,Corvallis,97331,United States; 3.Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	附属第二医院; 环境安全与健康风险评估研究院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Shen, Qiaosen,Truong, Lisa,Simonich, Michael T.,et al. Rapid well-plate assays for motor and social behaviors in larval zebrafish[J]. BEHAVIOURAL BRAIN RESEARCH,2020,391:112625.
APA	Shen, Qiaosen, Truong, Lisa, Simonich, Michael T., Huang, Changjiang, Tanguay, Robyn L., & Dong, Qiaoxiang. (2020). Rapid well-plate assays for motor and social behaviors in larval zebrafish. BEHAVIOURAL BRAIN RESEARCH, 391, 112625.
MLA	Shen, Qiaosen,et al."Rapid well-plate assays for motor and social behaviors in larval zebrafish".BEHAVIOURAL BRAIN RESEARCH 391(2020):112625.