Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells

doi:10.4238/gmr16029298

科研成果详情

题名	Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells
作者	Huang Y.D.1; Tong X.2; Chen Q.1; Xia S.W.1; Zhuo Y.1; Kang Z.H.1; Xue X.Y.3
发表日期	2017-05-16
发表期刊	GENETICS AND MOLECULAR RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Angioma C/Ebp-homologous protein Pingyangmycin Endoplasmic reticulum stress Glucose-regulated protein 78
其他关键词	ENDOPLASMIC-RETICULUM STRESS ; VENOUS MALFORMATIONS ; INFANTILE HEMANGIOMAS ; BLEOMYCIN A5 ; HEAD ; NECK ; SCLEROTHERAPY ; CLEAVAGE ; GROWTH
摘要	In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK-8) was used to detect growth inhibiting effects. Annexin V-FITC/propidium iodide stain was used to detect apoptosis, and western blot was used to detect the expression of glucose-related protein 78 (GPR78) and C/EBP homologous protein CHOP) endoplasmic reticulum stress proteins. With increasing PYM concentration, the growth inhibition of HUVEC increased (P < 0.05), the apoptotic numbers of HUVEC increased (P < 0.05), with higher PYM concentrations inducing necrosis, and the protein expression of GRP78 and CHOP increased (P < 0.05). PYM could obviously inhibit the proliferation and promote the apoptosis of HUVEC. Necrotic cells were more prevalent than apoptotic cells at high PYM concentrations. This study helped to determine the proper concentration of PYM to induce more apoptosis than necrosis, which is critical to minimize inflammation, enhance the healing of the skin, and maintain safety for the patient. PYM might induce HUVEC apoptosis through the endoplasmic reticulum stress pathway.
出版者	FUNPEC-EDITORA
出版地	RIBEIRAO PRETO
ISSN	1676-5680
卷号	16 期号:2
DOI	10.4238/gmr16029298
页数	9
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000417362200005
收录类别	ESCI ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28510249
SCOPUSEID	2-s2.0-85019606080
通讯作者地址	[Huang Y.D.]Department of Otorhinolaryngology Head & Neck Surgery of the First Clinical College of Wenzhou Medical University,The 118th Hospital of PLA,Wenzhou,China
Scopus学科分类	Molecular Biology;Genetics
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2523
专题	第一临床医学院（信息与工程学院）、附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Huang Y.D.
作者单位	1.Department of Otorhinolaryngology Head & Neck Surgery of the First Clinical College of Wenzhou Medical University,The 118th Hospital of PLA,Wenzhou,China; 2.Department of Otorhinolaryngology Head and Neck Surgery,Zhengzhou Central Hospital,Zhengzhou,China; 3.Department of Microbiology and Immunology of Wenzhou Medical College,Research Institute of Molecular Virology and Immunology,Wenzhou,China
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Huang Y.D.,Tong X.,Chen Q.,et al. Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells[J]. GENETICS AND MOLECULAR RESEARCH,2017,16(2).
APA	Huang Y.D.., Tong X.., Chen Q.., Xia S.W.., Zhuo Y.., ... & Xue X.Y.. (2017). Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells. GENETICS AND MOLECULAR RESEARCH, 16(2).
MLA	Huang Y.D.,et al."Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells".GENETICS AND MOLECULAR RESEARCH 16.2(2017).