Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis

doi:10.3788/CJL201845.0307015

科研成果详情

题名	Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis
其他题名	Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis
作者	Cai, Shuangshuang1,2; Zheng, Longfei 1,2; Zeng, Bixin 1,2; Li, Run 3; Xu, Min 2,4
发表日期	2018-03-01
发表期刊	CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG 影响因子和分区
语种	中文
原始文献类型	Article
关键词	medical optics quantitative phase imaging transport-of-intensity equation differential interference contrast microscope scattering phase theorem optical pathology
其他关键词	SCATTERING ; RETRIEVAL ; MIE
摘要	A quantitative phase imaging system capable of revealing three-dimensional changes of microstructure and light scattering properties of cancer tissues is developed based on the transport-of-intensity equation using the differential interference contrast (DIC) microscope. The DIC images of unstained paraffin embedded tissue sections of 257 patients with invasive ductal carcinoma are acquired with the quantitative phase imaging system. The corresponding two-dimensional phase maps are obtained with the calculation of the transport-of-intensity equation. The spatially resolved optical properties of the tissues are computed from the two-dimensional phase maps using the scattering-phase theorem afterwards. The results show that the grade of invasive ductal carcinoma has strong correlation with reduced scattering coefficients and anisotropy of tissues. When the grade of invasive ductal carcinoma increases, the reduced scattering coefficient of invasive ductal carcinoma tissues increases significantly, while the anisotropy decreases significantly. The retrieved optical parameters are shown to distinguish invasive ductal carcinoma with different grades with a high accuracy of 88%. Owing to the label-free and high-contrast characteristics of DIC microscope, the proposed approach is also applicable for fresh or frozen tissues, and may have promising applications in real-time and rapid cancer diagnosis.
其他摘要	A quantitative phase imaging system capable of revealing three-dimensional changes of microstructure and light scattering properties of cancer tissues is developed based on the transport-of-intensity equation using the differential interference contrast (DIC) microscope. The DIC images of unstained paraffin embedded tissue sections of 257 patients with invasive ductal carcinoma are acquired with the quantitative phase imaging system. The corresponding two-dimensional phase maps are obtained with the calculation of the transport-of-intensity equation. The spatially resolved optical properties of the tissues are computed from the two-dimensional phase maps using the scattering-phase theorem afterwards. The results show that the grade of invasive ductal carcinoma has strong correlation with reduced scattering coefficients and anisotropy of tissues. When the grade of invasive ductal carcinoma increases, the reduced scattering coefficient of invasive ductal carcinoma tissues increases significantly, while the anisotropy decreases significantly. The retrieved optical parameters are shown to distinguish invasive ductal carcinoma with different grades with a high accuracy of 88%. Owing to the label-free and high-contrast characteristics of DIC microscope, the proposed approach is also applicable for fresh or frozen tissues, and may have promising applications in real-time and rapid cancer diagnosis.
资助项目	国家自然科学基金 ; 浙江省自然科学基金重点项目 ; 温州市公益性科技计划
出版者	CHINESE LASER PRESS
出版地	SHANGHAI
ISSN	0258-7025
卷号	45 期号:3 页码:0307015-1-0307015-7
DOI	10.3788/CJL201845.0307015
页数	7
WOS类目	Optics
WOS研究方向	Optics
WOS记录号	WOS:000582555000030
收录类别	ESCI ; EI ; SCOPUS ; CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
EI入藏号	20183205654421
EI主题词	Tissue
学科领域	物理学
URL	查看原文
CSCD记录号	CSCD:6209728
SCOPUSEID	2-s2.0-85050864560
通讯作者地址	[Xu, Min]Institute of Lasers and Biomedical Photonics,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Electronic, Optical and Magnetic Materials;Atomic and Molecular Physics, and Optics;Electrical and Electronic Engineering
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2493
专题	眼视光学院（生物医学工程学院）、附属眼视光医院仁济学院_眼视光、生物医学工程学部
通讯作者	Xu, Min
作者单位	1.School of Biomedical Engineering,Wenzhou Medical University,Wenzhou,325035,China; 2.Institute of Lasers and Biomedical Photonics,Wenzhou Medical University,Wenzhou,325035,China; 3.Department of Mechanical Engineering,Fairfield University,CT,06824,United States; 4.Department of Physics,Fairfield University,CT,06824,United States
第一作者单位	眼视光、生物医学工程学部; 温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	眼视光、生物医学工程学部
推荐引用方式 GB/T 7714	Cai, Shuangshuang,Zheng, Longfei,Zeng, Bixin,et al. Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis[J]. CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG,2018,45(3):0307015-1-0307015-7.
APA	Cai, Shuangshuang, Zheng, Longfei, Zeng, Bixin, Li, Run, & Xu, Min. (2018). Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis. CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG, 45(3), 0307015-1-0307015-7.
MLA	Cai, Shuangshuang,et al."Quantitative Phase Imaging Based on Transport-of-Intensity Equation and Differential Interference Contrast Microscope and Its Application in Breast Cancer Diagnosis".CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG 45.3(2018):0307015-1-0307015-7.

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