A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells

doi:10.1016/j.talanta.2025.127622

科研成果详情

题名	A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells
作者	Hong, Yawen1; Xu, Lian1; Sun, Hengwei2; Wu, Wen1; Cai, Xiaojun 1; Lin, Qingfeng 3,4,5; Chen, Xiaoyang 3,4,5; Wang, Yanying 3,4,5; Li, Chunya 3,4,5; Qu, Jinmiao2; Sun, Dong1
发表日期	2025-05-15
发表期刊	TALANTA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Split-type biosensor Aptamer Photoelectrochemical sensing Colorimetric sensing HepG2 cells
其他关键词	AMPLIFICATION
摘要	Hepatocellular carcinoma (HCC) stands as a grave illness characterized by elevated death rates. Early identification plays a vital role in improving patient survival. Herein, a novel split-type dual-mode biosensor featuring with near-infrared photoelectronchemical (PEC) and colorimetric sensing characteristics was developed for the high-performance detection of HepG2 cells. Biotin labeled aptamer (Bio-Apt1) was immobilized onto 96-well plates functionalized with streptavidin to capture HepG2 cells through specific binding. HepG2 cells were then labeled with another aptamer (Apt-2) by recognizing GPC3 on the surface of HepG2 cells. Apt 2 could form DNA double strand (dsDNA-ALP) with ALP-labeled complementary DNA (cDNA-ALP). Subsequently, ALP was released to catalyze AAP to form ascorbic acid (AA), and AA reduced HAuCl4 to form gold nanoparticles (AuNPs). Then the mixture containing AuNPs was introduced onto the surface of Y-MOFs/GCE to enhance the photocurrent response. The change of photocurrent corresponding to the concentration of HepG2 cells can be used for the PEC determination. ALP can catalyze the hydrolysis of disodium phenyl phosphate to produce phenol, followed by a reaction with 4-aminoantipyrine and potassium ferricyanide, resulting in a quinone derivative for the colorimetric determination. The photoelectrochemical and colorimetric detection models show excellent selectivity and sensitivity in identifying HepG2 cells, exhibiting a linear reaction range from 1.0 x 102 to 1.0 x 106 cells mL- 1 and a detection limit of 13 cells mL- 1 and 51 cells mL- 1, respectively. The dual-mode split type biosensor avoided direct damage to biomolecules from high-energy light, and the independent signal transduction enabled the acquisition of reliable results.
资助项目	National Natural Science Foundation of China [22074160, 22474158, 21874157]; Wenzhou Science and Technology Planning Project [ZY2023024]; Fund for Scientific Research Platforms of South-Central Minzu University [PTZ24010]; Fund for Academic Innovation Teams of South-Central Minzu University [XTZ24010]; Open Project Funding of Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University [KLSAOFM2305]
出版者	ELSEVIER
ISSN	0039-9140
EISSN	1873-3573
卷号	287
DOI	10.1016/j.talanta.2025.127622
页数	9
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:001413169800001
收录类别	SCIE ; SCOPUS ; EI ; PUBMED
EI入藏号	20250417765791
EI主题词	Ascorbic acid
EI分类号	101.7 ; 103 ; 804.1 Organic Compounds ; 805 Chemical Engineering, General
URL	查看原文
PubMed ID	39874791
SCOPUSEID	2-s2.0-85216010603
通讯作者地址	[Sun, Dong]Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China. ; [Qu, Jinmiao]Wenzhou Med Univ, Affiliated Hosp 1, Dept thyroid Surg, Wenzhou 325035, Peoples R China. ; [Li, Chunya]South Cent Minzu Univ, Key Lab Catalysis & Energy Mat Chem, Minist Educ, Wuhan 430074, Peoples R China. ; [Li, Chunya]South Cent Minzu Univ, Hubei Key Lab Catalysis & Mat Sci, Wuhan 430074, Peoples R China. ; [Li, Chunya]South Cent Minzu Univ, Key Lab Analyt Chem State Ethn Affairs Commiss, Wuhan 430074, Peoples R China.
Scopus学科分类	Analytical Chemistry
SCOPUS_ID	SCOPUS_ID:85216010603
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/225561
专题	药学院（分析测试中心）第一临床医学院（信息与工程学院）、附属第一医院_外科学_甲状腺外科
通讯作者	Li, Chunya; Qu, Jinmiao; Sun, Dong
作者单位	1.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1, Dept thyroid Surg, Wenzhou 325035, Peoples R China; 3.South Cent Minzu Univ, Key Lab Catalysis & Energy Mat Chem, Minist Educ, Wuhan 430074, Peoples R China; 4.South Cent Minzu Univ, Hubei Key Lab Catalysis & Mat Sci, Wuhan 430074, Peoples R China; 5.South Cent Minzu Univ, Key Lab Analyt Chem State Ethn Affairs Commiss, Wuhan 430074, Peoples R China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）; 第一临床医学院（信息与工程学院）、附属第一医院_外科学_甲状腺外科
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Hong, Yawen,Xu, Lian,Sun, Hengwei,et al. A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells[J]. TALANTA,2025,287.
APA	Hong, Yawen., Xu, Lian., Sun, Hengwei., Wu, Wen., Cai, Xiaojun., ... & Sun, Dong. (2025). A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells. TALANTA, 287.
MLA	Hong, Yawen,et al."A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells".TALANTA 287(2025).