smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans

doi:10.3389/fmicb.2024.1507928

科研成果详情

题名	smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans
作者	Zhou, Jing 1,2; Ma, Qizhao 1,2; Liang, Jingou 1,2; Pan, Yangyang1,3; Chen, Yang 1,4; Yu, Shuxing 1,2; Liu, Yaqi 1,2; Zhang, Qiong 1,2; Li, Yuqing 1; Zou, Jing 1,2
发表日期	2025-01-17
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	dental caries Streptococcus mutans acetyltransferase biofilms water-insoluble extracellular polysaccharides ClpL
其他关键词	LYSINE ACETYLATION ; STRESS TOLERANCE ; VIRULENCE ; SYSTEM ; ACETYLOME ; CLPL
摘要	Streptococcus mutans is a key etiological agent in dental caries, owing to its strong ability to form biofilms through carbohydrate fermentation. Protein acetylation, facilitated by GNAT family acetyltransferases, plays a critical regulatory role in bacterial physiology, but its impact on S. mutans remains largely unexplored. In this study, we investigated the role of the GNAT family acetyltransferase encoded by smu_1558c in regulating the growth and biofilm formation of S. mutans. The deletion of smu_1558c resulted in impaired growth, reduced biofilm formation, and diminished synthesis of water-insoluble extracellular polysaccharides (EPS). Proteomic analysis revealed 166 differentially expressed proteins in the deletion mutant, with significant enrichment in pathways related to carbohydrate transport and metabolism, and translation. Notably, glucosyltransferases GtfB and GtfC, key enzymes in biofilm formation, were significantly downregulated in the deletion mutant, while ClpL, a Clp-like ATP-dependent protease involved in protein homeostasis under stress conditions, was highly upregulated. These findings highlight that acetyltransferase smu_1558c plays a crucial role in the growth, biofilm formation, and EPS synthesis of S. mutans through its regulation of carbohydrate transport and metabolism pathways, as well as stress response mechanisms. This study provides novel insights into the molecular mechanisms governing S. mutans pathogenicity and suggests potential therapeutic targets for caries prevention.
资助项目	National Natural Science Foundation of China [32170046, 82170947, 32400094]; Sichuan Science and Technology Program [23ZDYF2052]; Natural Science Foundation of Sichuan Province [2023NSFSC1508]; Fundamental Research Funds for the Central Universities, Research and Develop Program, West China Hospital of Stomatology Sichuan University [RD-02-202402, RD-03-202402, RD-02-202210]; West China School/Hospital of Stomatology Sichuan University [RCDWJS2023-10]
出版者	FRONTIERS MEDIA SA
ISSN	1664-302X
EISSN	1664-302X
卷号	15
DOI	10.3389/fmicb.2024.1507928
页数	11
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001409799500001
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	39895941
通讯作者地址	[Li, Yuqing;Zou, Jing]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China. ; [Zou, Jing]Sichuan Univ, West China Hosp Stomatol, Dept Pediat Dent, Chengdu, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/225522
专题	口腔医学院、附属口腔医院_儿童口腔
通讯作者	Li, Yuqing; Zou, Jing
作者单位	1.Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China; 2.Sichuan Univ, West China Hosp Stomatol, Dept Pediat Dent, Chengdu, Peoples R China; 3.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Pediat Dent, Wenzhou, Peoples R China; 4.Peking Univ, Sch & Hosp Stomatol, Dept Pediat Dent, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jing,Ma, Qizhao,Liang, Jingou,et al. smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans[J]. FRONTIERS IN MICROBIOLOGY,2025,15.
APA	Zhou, Jing., Ma, Qizhao., Liang, Jingou., Pan, Yangyang., Chen, Yang., ... & Zou, Jing. (2025). smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans. FRONTIERS IN MICROBIOLOGY, 15.
MLA	Zhou, Jing,et al."smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans".FRONTIERS IN MICROBIOLOGY 15(2025).