Accelerating thrombolysis of arterial thrombus with NO-MBs UTMD therapy

doi:10.1016/j.ejpb.2024.114566

科研成果详情

题名	Accelerating thrombolysis of arterial thrombus with NO-MBs UTMD therapy
作者	Shi, Binbin 1; Yang, Qiaohua 2; Liang, Zenghui 3; Yu, Runjie 4; Li, Hui 1; Wu, Qilong1; Fang, Mingling 1; Lin, Lili 3; Chen, Huafang5; Zhao, Yingzheng6; Chen, Bin 1,2
发表日期	2024-12
发表期刊	EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Nitric oxide Lipid microbubble UTMD Arterial thrombotic disease Thrombolysis
其他关键词	NITRIC-OXIDE ; ENDOTHELIAL-CELLS ; ULTRASOUND ; MICROBUBBLES ; DELIVERY ; ISCHEMIA ; HYPOXIA ; INHIBITION
摘要	Arterial thrombotic disease is a common and serious clinical medical problem. Nitric oxide (NO), as a therapeutic gas, can delay the progression of thrombosis and reduce tissue ischemia and hypoxia damage. However, systemic delivery of NO causes complications, and NO in the body is easily cleared by hemoglobin in the blood. In this study, we designed a lipid microbubble carrying NO (NO-MBs) combined with ultrasound-targeted microbubble destruction (UTMD) technology to achieve targeted delivery of NO under real-time contrast-enhanced ultrasound monitoring. The good stability of the NO-MBs was demonstrated by examining the changes in diameter, concentration and contrast-enhanced ultrasound intensity with time. Moreover, in vivo and in vitro thrombolysis experiments, it was confirmed that the combination of NO-MBs and UTMD could accelerate arterial thrombolysis. Meanwhile, the levels of inflammatory factors, superoxide dismutase (SOD) and malondialdehyde (MDA) in vascular tissue after treatment were detected, which showed that NO-MBs could significantly reduce the inflammatory response and oxidative stress induced by thromboembolism. In addition, so as to evaluate the safety of the NO-MBs UTMD treatment strategy, MTT assay, hemolysis test, detection of serum biochemical indicators, and H&E staining of major organs were performed. The results showed that this treatment strategy had excellent biosafety. In conclusion, the NO-MBs UTMD treatment strategy has great potential in the treatment of arterial thrombotic diseases.
资助项目	Zhejiang provincial program for the cultivation of high-level innovative health talents [LY19H180001, LQ19H300001, LY20H300002]; Wenzhou innovative technology project for high-level talents; [Y20190177]; [Y20180183]
出版者	ELSEVIER
ISSN	0939-6411
EISSN	1873-3441
卷号	205
DOI	10.1016/j.ejpb.2024.114566
页数	14
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:001350292800001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39477029
SCOPUSEID	2-s2.0-85207788206
通讯作者地址	[Chen, Bin]Wenzhou Med Univ, Affiliated Hosp 1, Dept Ultrasonog, Wenzhou, Zhejiang, Peoples R China. ; [Chen, Huafang;Zhao, Yingzheng;Chen, Bin]Shenzhen Univ, Affiliated Hosp 1, Peoples Hosp Shenzhen 2, Dept Ultrasound, Shenzhen, Guangdong, Peoples R China.
Scopus学科分类	Biotechnology;Pharmaceutical Science
SCOPUS_ID	SCOPUS_ID:85207788206
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/221265
专题	附属第一医院药学院（分析测试中心）_药学系附属第一医院_药物临床试验机构办公室
通讯作者	Chen, Huafang; Zhao, Yingzheng; Chen, Bin
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Ultrasonog, Wenzhou, Zhejiang, Peoples R China; 2.Shenzhen Univ, Affiliated Hosp 1, Peoples Hosp Shenzhen 2, Dept Med Ultrasound, Shenzhen, Guangdong, Peoples R China; 3.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Ultrasonog, Hangzhou, Zhejiang, Peoples R China; 4.Zhejiang Lab Regenerat Med Vis & Brain Hlth, Oujiang Lab, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 1, Off Drug Clin Trial Inst, Wenzhou, Zhejiang, Peoples R China; 6.Wenzhou Med Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Shi, Binbin,Yang, Qiaohua,Liang, Zenghui,et al. Accelerating thrombolysis of arterial thrombus with NO-MBs UTMD therapy[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2024,205.
APA	Shi, Binbin., Yang, Qiaohua., Liang, Zenghui., Yu, Runjie., Li, Hui., ... & Chen, Bin. (2024). Accelerating thrombolysis of arterial thrombus with NO-MBs UTMD therapy. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 205.
MLA	Shi, Binbin,et al."Accelerating thrombolysis of arterial thrombus with NO-MBs UTMD therapy".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 205(2024).