Novel anti-CEA affibody for rapid tumor-targeting and molecular imaging diagnosis in mice bearing gastrointestinal cancer cell lines

doi:10.3389/fmicb.2024.1464088

科研成果详情

题名	Novel anti-CEA affibody for rapid tumor-targeting and molecular imaging diagnosis in mice bearing gastrointestinal cancer cell lines
作者	Huanyi Shao1,2; Kaiji Lv 3; Pengfei Wang3; Jinji Jin 3; Yiqi Cai3; Jun Chen2; Saidu Kamara2; Shanli Zhu2; Guanbao Zhu3; Lifang Zhang2
发表日期	2024
发表期刊	Frontiers in microbiology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	CEA affibody molecules gastrointestinal cancer molecular imaging tumor diagnosis
其他关键词	HUMAN CARCINOEMBRYONIC ANTIGEN ; PROTEIN SECONDARY STRUCTURE ; GROWTH-FACTOR ; CDNA-CLONES ; PREDICTION ; EXPRESSION ; FAMILY ; TOOLS
摘要	Gastrointestinal cancer is a common malignant tumor with a high incidence worldwide. Despite continuous improvements in diagnosis and treatment strategies, the overall prognosis of gastrointestinal tumors remains poor. Carcinoembryonic antigen (CEA) is highly expressed in various types of cancers, especially in gastrointestinal cancers, making it a potential target for therapeutic intervention. Therefore, the expression of CEA can be used as an indication of the existence of tumors, chosen as a target for molecular imaging diagnosis, and effectively utilized in the targeted therapy of gastrointestinal cancers. In this study, we report the selection and characterization of affibody molecules (ZCEA539, ZCEA546, and ZCEA919) specific to the CEA protein. Their ability to bind to recombinant and native CEA protein has been confirmed by surface plasmon resonance (SPR), immunofluorescence, and immunohistochemistry assays. Furthermore, Dylight755-labeled ZCEA affibody showed accumulation within the tumor site 1 h post injection and was continuously enhanced for 4 h. The Dylight755-labeled ZCEA affibody exhibited high tumor-targeting specificity in CEA+ xenograft-bearing mice and possesses promising characteristics for tumor-targeting imaging. Overall, our results suggest the potential use of ZCEA affibodies as fluorescent molecular imaging probes for detecting CEA expression in gastrointestinal cancer
资助项目	Public Welfare Foundation of Zhejiang Province [LGF18H160030]
出版者	FRONTIERS MEDIA SA
ISSN	1664-302X
EISSN	1664-302X
卷号	15
DOI	10.3389/fmicb.2024.1464088
页数	9
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001337952600001
收录类别	PUBMED ; SCIE
URL	查看原文
PubMed ID	39444679
通讯作者地址	[Zhang, Lifang]Wenzhou Med Univ, Inst Mol Virol & Immunol, Sch Basic Med Sci, Dept Microbiol & Immunol, Wenzhou, Peoples R China. ; [Zhu, Guanbao]Wenzhou Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Wenzhou, Peoples R China.
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/220992
专题	附属第一医院附属第一医院_胃肠外科基础医学院（机能实验教学中心）_病原生物学与免疫学系
作者单位	1.Department of Pediatric Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.; 2.Department of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, China.; 3.Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
第一作者单位	附属第一医院; 基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系; 附属第一医院_胃肠外科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huanyi Shao,Kaiji Lv,Pengfei Wang,et al. Novel anti-CEA affibody for rapid tumor-targeting and molecular imaging diagnosis in mice bearing gastrointestinal cancer cell lines[J]. Frontiers in microbiology,2024,15.
APA	Huanyi Shao., Kaiji Lv., Pengfei Wang., Jinji Jin., Yiqi Cai., ... & Lifang Zhang. (2024). Novel anti-CEA affibody for rapid tumor-targeting and molecular imaging diagnosis in mice bearing gastrointestinal cancer cell lines. Frontiers in microbiology, 15.
MLA	Huanyi Shao,et al."Novel anti-CEA affibody for rapid tumor-targeting and molecular imaging diagnosis in mice bearing gastrointestinal cancer cell lines".Frontiers in microbiology 15(2024).