Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response

doi:10.1016/j.riam.2024.04.002

科研成果详情

题名	Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response
作者	Yu, Wenwen; Ye, Hua; Li, Yunlei; Bao, Xiaoqiong; Ni, Yangyang; Chen, Xiangxiang; Sun, Yangjie; Chen, Ali; Zhou, Weilong; Li, Jifa
发表日期	2024-03
发表期刊	Revista iberoamericana de micologia 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Fibrosis pulmonar Fn1 Infección Infection Inflamación Inflammation Pneumocystis carinii Pulmonary fibrosis
其他关键词	FIBRONECTIN ; POLYMERIZATION
摘要	Pneumocystis carinii is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis., This study attempted to investigate the role of P. carinii infection-related genes in regulating lung fibrosis in mice., A screening of P. carinii infection-related differential mRNAs was performed using the GEO database, followed by protein-protein interaction (PPI) network construction using the STRING website in order to obtain P. carinii infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin-eosin (H&E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation., {AbstractText=Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in P. carinii-infected mice samples. The P carinii pneumonia (PCP)+Fn1fl/fl group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1fl/fl group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1fl/fl group had the most intense H&E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1fl/fl group.}, P. carinii infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.
出版者	ASOCIACION ESPANOLA MICOLOGIA-AEM
ISSN	1130-1406
EISSN	2173-9188
卷号	41 期号:1 页码:17-26
DOI	10.1016/j.riam.2024.04.002
页数	10
WOS类目	Mycology
WOS研究方向	Mycology
WOS记录号	WOS:001331508100001
收录类别	PUBMED ; SCIE
URL	查看原文
PubMed ID	39332977
通讯作者地址	[Li, Jifa]Wenzhou Med Univ, Affiliated Yueqing Hosp, Dept Resp & Crit Care Med, Wenzhou, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218992
专题	其他_附属乐清医院（乐清市人民医院）
通讯作者	Li, Jifa
作者单位	Wenzhou Med Univ, Affiliated Yueqing Hosp, Dept Resp & Crit Care Med, Wenzhou, Zhejiang, Peoples R China
第一作者单位	其他_附属乐清医院（乐清市人民医院）
通讯作者单位	其他_附属乐清医院（乐清市人民医院）
第一作者的第一单位	其他_附属乐清医院（乐清市人民医院）
推荐引用方式 GB/T 7714	Yu, Wenwen,Ye, Hua,Li, Yunlei,et al. Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response[J]. Revista iberoamericana de micologia,2024,41(1):17-26.
APA	Yu, Wenwen., Ye, Hua., Li, Yunlei., Bao, Xiaoqiong., Ni, Yangyang., ... & Li, Jifa. (2024). Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response. Revista iberoamericana de micologia, 41(1), 17-26.
MLA	Yu, Wenwen,et al."Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response".Revista iberoamericana de micologia 41.1(2024):17-26.