Depression exacerbates myocardial ischemia-reperfusion injury in mice via CNR2 gene and MIF-AMPK signaling pathway

doi:10.1016/j.ijcard.2024.132505

科研成果详情

题名	Depression exacerbates myocardial ischemia-reperfusion injury in mice via CNR2 gene and MIF-AMPK signaling pathway
作者	Qian, Lu; Zhang, Suqin; Lin, Cong; Lin, Jiafeng; Li, Zhuoyuan
发表日期	2024-12-01
发表期刊	International Journal of Cardiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Depression Ischemia-reperfusion injury Cannabinoid receptor 2 Macrophage migration inhibitory factor AMP-activated protein kinase
其他关键词	MIGRATION INHIBITORY FACTOR ; ACTIVATED PROTEIN-KINASE ; EXPRESSION ; REGULATOR ; UNIQUE
摘要	Background: Myocardial ischemia-reperfusion(I/R)injury constitute the fundamental pathophysiology of acute myocardial infarction (AMI). Ischemic heart releases macrophage migration inhibitory factor (MIF), which activates MIF- AMPK signaling pathway. Depression is a significant risk factor for AMI. In a state of depression, peripheral expression of cannabinoid receptor 2 (CNR2) genes was downregulated. Aims: We investigated the mechanism by which depression exacerbates myocardial I/R injury through the CNR2 and MIF-AMPK signaling pathways. Methods: We established mouse models of depression and myocardial I/R. Left ventricular function was assessed using cardiac ultrasound and TTC staining. The protein levels of myocardial CNR2, MIF, AMPK, and ACC were determined by Western blot, while the expression level of CNR2 was measured using RT-qPCR. Additionally, MIF content in peripheral blood was quantified using ELISA. Results: After I/R, the expression level of CNR2 was found to be lower in the depression group, leading to a deterioration in left heart function. Depressed mice exhibited lower secretion of MIF, accompanied by a decrease in the activation of the MIF-AMPK signaling pathway. However, injection of CNR2 agonist JWH133 prior to ischemia increased the activation of the MIF-AMPK signaling pathway, while CNR2 inhibitor AM630 decreased the activation. Limitations: Further research is needed to investigate the specific neuroendocrine mechanism affecting myocardial CNR2 expression in depression. And these experimental conclusions require further verification at the cellular level. Conclusions: The activation of CNR2 in myocardium following I/R is impeded by depression, thereby exacerbating myocardial I/R injury through attenuation of the MIF-AMPK signaling pathway activation.
资助项目	Basic Research Project of Wenzhou City [Y20220471, Y20220390]
出版者	ELSEVIER IRELAND LTD
ISSN	0167-5273
EISSN	1874-1754
卷号	416
DOI	10.1016/j.ijcard.2024.132505
页数	9
WOS类目	Cardiac & Cardiovascular Systems
WOS研究方向	Cardiovascular System & Cardiology
WOS记录号	WOS:001309600000001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39222886
SCOPUSEID	2-s2.0-85203014771
通讯作者地址	[Lin, Jiafeng;Li, Zhuoyuan]Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiol, 109 Xueyuan Rd, Wenzhou 325000, Zhejiang, Peoples R China.
Scopus学科分类	Cardiology and Cardiovascular Medicine
SCOPUS_ID	SCOPUS_ID:85203014771
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218750
专题	附属第二医院附属第二医院_耳鼻咽喉科第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Lin, Jiafeng; Li, Zhuoyuan
作者单位	Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiol, 109 Xueyuan Rd, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 耳鼻咽喉科
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 耳鼻咽喉科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Qian, Lu,Zhang, Suqin,Lin, Cong,et al. Depression exacerbates myocardial ischemia-reperfusion injury in mice via CNR2 gene and MIF-AMPK signaling pathway[J]. International Journal of Cardiology,2024,416.
APA	Qian, Lu, Zhang, Suqin, Lin, Cong, Lin, Jiafeng, & Li, Zhuoyuan. (2024). Depression exacerbates myocardial ischemia-reperfusion injury in mice via CNR2 gene and MIF-AMPK signaling pathway. International Journal of Cardiology, 416.
MLA	Qian, Lu,et al."Depression exacerbates myocardial ischemia-reperfusion injury in mice via CNR2 gene and MIF-AMPK signaling pathway".International Journal of Cardiology 416(2024).