Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques

doi:10.3389/fendo.2024.1423801

科研成果详情

题名	Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques
作者	Huang, Fu 1,2,3; Wang, Jiexia1,2,3; Wang, Hu4; Hu, Yun1,2,3; Li, Zhenni4; Xu, Jingfeng5; Qin, Mengjie4; Wen, Xin1,2,3; Cao, Shuyan6; Guan, Xiaoju1,2; Duan, Ping3; Chen, Haolin1,2,3,4,5; Chen, Congde1,2
发表日期	2024-08-20
发表期刊	FRONTIERS IN ENDOCRINOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	testicular interstitial cells rat Leydig cells scRNA-seq mesenchymal cells EDS hemicastration
其他关键词	DIMETHANE SULFONATE ; ETHANE DIMETHANE ; MACROPHAGES ; TESTIS ; SPERMATOGENESIS ; TESTOSTERONE ; EXPRESSION ; RATS ; GENE ; DIFFERENTIATION
摘要	Background The mammalian testicular interstitial cells are not well-defined. The present study characterized the interstitial cell types and their turnover dynamics in adult rats. Additionally, the heterogeneity of the mesenchymal population and the effects of Leydig cell elimination on interstitial homeostasis were further analyzed by scRNA-seq datasets and immunocytochemical techniques. Methods Interstitial cells were defined at the transcriptomic level by scRNA-seq and then confirmed and quantified with protein markers. The dividing activity of the major cell types was determined by continuous EdU labeling of the animals for one week. Some of the rats were also treated with a dose of ethylenedimethylsulfonate (EDS) to examine how the loss of Leydig cells (LCs) could affect interstitial homeostasis for three weeks. Results Seven interstitial cell types were identified, including cell types (percentage of the whole interstitial population) as follows: Leydig (44.6%), macrophage and dendritic (19.1%), lymphoid (6.2%), vascular endothelial (7.9%), smooth muscle (10.7%), and mesenchymal (11.5%) cells. The EdU experiment indicated that most cell types were dividing at relatively low levels (<9%) except for the mesenchymal cells (MCs, 17.1%). Further analysis of the transcriptome of MCs revealed 4 subgroups with distinct functions, including 1) glutathione metabolism and xenobiotic detoxification, 2) ROS response and AP-1 signaling, 3) extracellular matrix synthesis and binding, and 4) immune response and regulation. Stem LCs (SLCs) are primarily associated with subgroup 3, expressing ARG1 and GAP43. EDS treatment not only eliminated LCs but also increased subgroup 3 and decreased subgroups 1 and 2 of the mesenchymal population. Moreover, EDS treatment increased the division of immune cells by more than tenfold in one week. Conclusion Seven interstitial cell types were identified and quantified for rat testis. Many may play more diversified roles than previously realized. The elimination of LCs led to significant changes in MCs and immune cells, indicating the importance of LCs in maintaining testicular interstitial homeostasis.
资助项目	Wenzhou Municipal Science and Technology Bureau, CN [ZY2019002, ZY2020009]; Zhejiang Public Welfare Technology Application Research Project [LGF21H040010]; Natural Science Foundation of Zhejiang Province, CN [Z23H040003, Q23H040007]
出版者	FRONTIERS MEDIA SA
ISSN	1664-2392
卷号	15
DOI	10.3389/fendo.2024.1423801
页数	19
WOS类目	Endocrinology & Metabolism
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:001303722100001
收录类别	SCIE
PubMed ID	39229372
通讯作者地址	[Chen, Haolin;Chen, Congde]Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Pediat Urol, Key Lab Children Genitourinary Dis Wenzhou City, Wenzhou, Zhejiang, Peoples R China. ; [Chen, Haolin;Chen, Congde]Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Key Lab Struct Malformat Children Zhejiang Prov, Dept Pediat Urol, Wenzhou, Zhejiang, Peoples R China. ; [Chen, Haolin]Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Gynecol & Obstet, Wenzhou, Zhejiang, Peoples R China. ; [Chen, Haolin]Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Pharmacol, Wenzhou, Zhejiang, Peoples R China. ; [Chen, Haolin]Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Anesthesiol, Zhejiang Prov Key Lab Anesthesiol, Wenzhou, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218747
专题	第二临床医学院，附属第二医院、育英儿童医院第二临床医学院，附属第二医院、育英儿童医院_妇产科学第二临床医学院，附属第二医院、育英儿童医院_麻醉学_麻醉科
通讯作者	Chen, Haolin; Chen, Congde
作者单位	1.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Pediat Urol, Key Lab Children Genitourinary Dis Wenzhou City, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Key Lab Struct Malformat Children Zhejiang Prov, Dept Pediat Urol, Wenzhou, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Gynecol & Obstet, Wenzhou, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Pharmacol, Wenzhou, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Anesthesiol, Zhejiang Prov Key Lab Anesthesiol, Wenzhou, Zhejiang, Peoples R China; 6.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Basic Med Res Ctr, Sch Med 2, Wenzhou, Zhejiang, Peoples R China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 第二临床医学院，附属第二医院、育英儿童医院_妇产科学
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 第二临床医学院，附属第二医院、育英儿童医院_妇产科学; 第二临床医学院，附属第二医院、育英儿童医院_麻醉学_麻醉科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Huang, Fu,Wang, Jiexia,Wang, Hu,et al. Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques[J]. FRONTIERS IN ENDOCRINOLOGY,2024,15.
APA	Huang, Fu., Wang, Jiexia., Wang, Hu., Hu, Yun., Li, Zhenni., ... & Chen, Congde. (2024). Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques. FRONTIERS IN ENDOCRINOLOGY, 15.
MLA	Huang, Fu,et al."Effects of Leydig cell elimination on testicular interstitial cell populations: characterization by scRNA-seq and immunocytochemical techniques".FRONTIERS IN ENDOCRINOLOGY 15(2024).