Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc

doi:10.7717/peerj.18040

科研成果详情

题名	Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc
作者	Liang, Feng 1; Sun, Shujuan 2; Zhou, YongGuang3; Peng, Tiantian3; Xu, Xianxian3; Li, Beibei3; Tan, Guoqiang3
发表日期	2024-09-10
发表期刊	PEERJ 影响因子和分区
语种	英语
原始文献类型	Article
关键词	YahK Iron ions Zinc ions Alcohol dehydrogenase NADP plus
其他关键词	ROLES ; K-12 ; MDR
摘要	Background: Previous studies have highlighted the catalytic activity of Escherichia coli alcohol dehydrogenase YahK in the presence of coenzyme nicotinamide adenine dinucleotide (NAD) and metal zinc. Notably, competitive interaction between iron and zinc ligands has been shown to influence the catalytic efficiency of several key proteases. This study aims to unravel the intricate mechanisms underlying YahK's catalytic action, with a particular focus on the pivotal roles played by metal ions zinc and iron. Methods: The purified YahK protein from E. coli cells cultivated in LB medium was utilized to investigate its metal-binding properties through UV-visible absorption measurements and determination of metal content. Subsequently, the effects of excess zinc and iron on the metal-binding ability and alcohol dehydrogenase activity of the YahK protein were explored using M9 minimal medium. Furthermore, site-directed mutagenesis technology was employed to determine the iron-binding site location within the YahK protein. Polyacrylamide gel electrophoresis was conducted to examine the relationship between iron and zinc with respect to the YahK protein. Results: The study confirmed the presence of iron and zinc in the YahK protein, with the zinc-bound form exhibiting enhanced catalytic activity in alcohol dehydrogenation reactions. Conversely, the presence of iron appears to play a pivotal role in maintaining overall stability of the YahK protein. Furthermore, experimental findings indicate that excessive zinc within M9 minimal medium can competitively bind to iron-binding sites on YahK, thereby augmenting its alcohol dehydrogenase activity. Conclusion: The dynamic binding of YahK to iron and zinc unveils its intricate regulatory mechanism as an alcohol dehydrogenase, thereby highlighting the possible physiological role of YahK in E. coli and its significance in governing cellular metabolic processes. This discovery provides a novel perspective for further investigating the specific impact of metal ion binding on YahK and E. coli cell metabolism.
资助项目	Wenzhou Municipal Science and Technology Bureau of China [Y20220743]; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province [2022E10022]
出版者	PEERJ INC
ISSN	2167-8359
卷号	12
DOI	10.7717/peerj.18040
页数	18
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:001316376400006
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	39282118
通讯作者地址	[Tan, Guoqiang]Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Technol & Applicat Model Org, Lab Mol Med,Key Lab Lab Med,Minist Educ, Wenzhou, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218689
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Tan, Guoqiang
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Clin Lab, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Wenzhou, Zhejiang, Peoples R China; 2.Linyi Univ, Coll Med, Shandong Prov Key Lab Detect Technol Tumor Markers, Linyi, Shandong, Peoples R China; 3.Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Technol & Applicat Model Org, Lab Mol Med,Key Lab Lab Med,Minist Educ, Wenzhou, Zhejiang, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 模式生物技术与应用重点实验室
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liang, Feng,Sun, Shujuan,Zhou, YongGuang,et al. Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc[J]. PEERJ,2024,12.
APA	Liang, Feng., Sun, Shujuan., Zhou, YongGuang., Peng, Tiantian., Xu, Xianxian., ... & Tan, Guoqiang. (2024). Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc. PEERJ, 12.
MLA	Liang, Feng,et al."Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc".PEERJ 12(2024).