Anti-Inflammatory, Analgesic and Anti-Oxidant Effects of Shirakiopsis Indica (Willd). Fruit Extract: A Mangrove Species in the Field of Inflammation Research

doi:10.2147/JIR.S470835

科研成果详情

题名	Anti-Inflammatory, Analgesic and Anti-Oxidant Effects of Shirakiopsis Indica (Willd). Fruit Extract: A Mangrove Species in the Field of Inflammation Research
作者	Jiko, Pair Ahmed ; Mohammad, Mahathir ; Richi, Fahmida Tasnim 1; Islam, Md. Anisul 2; Alam, Safaet 1,3; Taher, Mohammad Abdullah 1,4; Shao, Chuxiao 5; Wang, Shuanghu 5; Geng, Peiwu 5; Al Mamun, Abdullah 5,6
发表日期	2024
发表期刊	JOURNAL OF INFLAMMATION RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	natural products medicinal plants shirakiopsis indica acute oral toxicity analgesic anti-inflammatory antioxidant GC- MS/MS molecular docking
其他关键词	HELICOBACTER-PYLORI ; MOLECULAR DOCKING ; FORMALIN TEST ; DRUGS ; EDEMA ; MICE ; TOOL
摘要	Background: Shirakiopsis indica (Willd)., commonly known as Sa-Mor-Ta-Lay in Thailand, is a mangrove plant belonging to the Euphorbiaceae family. As mangrove plants' medicinal potentials are less explored, this study sought to qualitatively and quantitatively verify the bioactive components of Shirakiopsis indica fruits methanolic extract (SIF-ME) at the side of its analgesic, antiinflammatory and anti-oxidant effects followed by in-silico studies. Methods: The in-vivo assessments of analgesic activity involved the hot plate test, acetic acid-induced writhing test, and formalin-induced licking test. The anti-inflammatory efficacy was assessed through the human RBC membrane stabilization assay (HRBC), protein denaturation assay, and xylene-induced ear edema methods. Antioxidant potential was implemented by the DPPH scavenging method. Results: The SIF-ME consistently displayed significant anti-nociceptive activity in a dose-dependent pattern (p < 0.05). The maximum analgesic activity was found in the highest dose (200 mg/kg; p < 0.001) in a hot plate, acetic acid-induced writhing test 43.47%, and in formalin-induced licking test in both early phase (43.3%; p < 0.01) and late phase (61.84%; p < 0.001%). The extract provided optimal protection against hemolysis (83.41% decrease) at 1000 mu g/mL and significantly inhibited protein denaturation (67.34-26.05%) at doses of 1000-62.5 mu g/mL. At 200 mg/kg, the extract showed dose-dependent and substantial inhibition (54.07%; p < 0.01) of xylene-induced ear edema. The in-vitro DPPH (IC50 50 = 469.5 g/mL) results showed remarkable scavenging activity and concentration-dependent reducing power. The extract demonstrates no acute oral toxicity, as indicated by an LD50 50 value exceeding 1000 mg/kg body weight. Gas Chromatography-Mass Spectrometry/Mass Spectrometry (GC-MS/MS) analysis was performed which yielded sixty bioactive compounds. In-silico and molecular docking studies revealed favorable pharmacological properties, including good binding affinities and ADME/T profiles. Conclusion: These results support the medicinal use of the plant, which makes it a potential source of analgesic, anti-inflammatory, and antioxidant candidates.
资助项目	Key Research & Development Plan of Zhejiang Province [2024C03171]; Zhejiang Provincial Traditional Chinese Medicine Science and Technology Project [2024ZL1302]; Post-Doctoral Research Start-Up Fund of Lishui People's Hospital [2023bsh001]
出版者	DOVE MEDICAL PRESS LTD
EISSN	1178-7031
卷号	17 页码:5821-5854
DOI	10.2147/JIR.S470835
页数	34
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:001305929500001
收录类别	SCIE
PubMed ID	39228677
通讯作者地址	[Al Mamun, Abdullah]Wenzhou Med Univ, Lishui Univ, Lishui Peoples Hosp, Cent Lab,Lishui Hosp,Affiliated Hosp 1, Lishui 323000, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218579
专题	附属第一医院其他_附属第六医院（丽水市人民医院）
通讯作者	Al Mamun, Abdullah
作者单位	1.Chittagong Univ Engn & Technol, Dept Chem, Chittagong 4349, Bangladesh; 2.Univ Dhaka, Fac Pharm, Dept Pharmaceut Chem, Dhaka 1000, Bangladesh; 3.BGC Trust Univ Bangladesh, Dept Pharm, Chittagong 4202, Bangladesh; 4.Bangladesh Council Sci & Ind Res BCSIR, Chem Res Div, BCSIR Dhaka Labs, Dhaka 1205, Bangladesh; 5.Bangladesh Reference Inst Chem Measurements BRiCM, Lab Rd, Dhaka 1205, Bangladesh; 6.Wenzhou Med Univ, Lishui Univ, Lishui Peoples Hosp, Cent Lab,Lishui Hosp,Affiliated Hosp 1, Lishui 323000, Zhejiang, Peoples R China
通讯作者单位	附属第一医院; 附属第六医院（丽水市人民医院）
推荐引用方式 GB/T 7714	Jiko, Pair Ahmed,Mohammad, Mahathir,Richi, Fahmida Tasnim,et al. Anti-Inflammatory, Analgesic and Anti-Oxidant Effects of Shirakiopsis Indica (Willd). Fruit Extract: A Mangrove Species in the Field of Inflammation Research[J]. JOURNAL OF INFLAMMATION RESEARCH,2024,17:5821-5854.
APA	Jiko, Pair Ahmed., Mohammad, Mahathir., Richi, Fahmida Tasnim., Islam, Md. Anisul., Alam, Safaet., ... & Al Mamun, Abdullah. (2024). Anti-Inflammatory, Analgesic and Anti-Oxidant Effects of Shirakiopsis Indica (Willd). Fruit Extract: A Mangrove Species in the Field of Inflammation Research. JOURNAL OF INFLAMMATION RESEARCH, 17, 5821-5854.
MLA	Jiko, Pair Ahmed,et al."Anti-Inflammatory, Analgesic and Anti-Oxidant Effects of Shirakiopsis Indica (Willd). Fruit Extract: A Mangrove Species in the Field of Inflammation Research".JOURNAL OF INFLAMMATION RESEARCH 17(2024):5821-5854.