Sustained-release of PDGF from PLGA microsphere embedded thermosensitive hydrogel promoting wound healing by inhibiting autophagy

doi:10.1016/j.jddst.2019.101405

科研成果详情

题名	Sustained-release of PDGF from PLGA microsphere embedded thermosensitive hydrogel promoting wound healing by inhibiting autophagy
作者	Xu, Ke 1,2; An, Ningchen 2; Zhang, Haijuan2; Zhang, Qilu 2; Zhang, Kailun 1; Hu, Xinying2; Wu, Yanqing1,2; Wu, Fangfang 2; Xiao, Jian2; Zhang, Hongyu2; Peng, Renyi 1; Li, Hao 3; Jia, Chang4
发表日期	2020-02
发表期刊	JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	PDGF PLGA microspheres Proliferation Migration Autophagy
其他关键词	ENDOTHELIAL GROWTH-FACTOR ; DRUG-DELIVERY ; CELLS ; ANGIOGENESIS ; REPAIR ; BFGF
摘要	Growth factors (GFs) act as important active molecules for the treatment of skin wounds. However, their clinical translation is seriously restrained due to single function and short half-life. Besides, the underlying GFs-mediated wound healing mechanism remains elusive. Therefore, we aimed to develop a thermo-sensitive chitosan hydrogel embedding with poly lactic-co-glycolic acid (PLGA) microspheres which can deliver platelet-derived growth factor receptor (PDGF) for wound healing. The generated PDGF-PLGA hydrogel was systematically evaluated for its wound healing efficiency by the in vivo experiments, including biocompatibility testing, cell proliferation and migration, wound closure rate, granulation tissue formation and collagen deposition. Further, the expression levels of autophagy-related proteins were detected. The results showed that the PDGF-PLGA hydrogel exhibited excellent cell compatibility, cell proliferation and migration. The PDGF-PLGA hydrogel was superior to PDGF alone and control groups in promoting wound closure, granulation tissue formation and collagen synthesis. In addition, the autophagy levels were significantly decreased by PDGF hydrogel in wound tissue of treated mice as compared with PDGF alone and control groups. We also found that the autophagy activator rapamycin delayed PDGF hydrogel-mediated wound healing. This study provides a novel mechanism for the beneficial effects of PDGF-PLGA hydrogel on the wound repair.
资助项目	Zhejiang Provincial Natural Science [LQ18H150003, LQ18C010003, LY19H150001, LQ18H090011]; National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81802251, 81772450, 81801233]; Wenzhou Science & Technology Bureau Foundation [Y20190064]; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [YKFJ3-011]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	1773-2247
EISSN	2588-8943
卷号	55
DOI	10.1016/j.jddst.2019.101405
页数	7
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000518386500054
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85075320655
通讯作者地址	[Jia, Chang]Pediatric Research Institute,The Second Affiliated Hospital and Yuying Children’ s Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Pharmaceutical Science
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2175
专题	药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院研究生工作部（研究生院）其他_附属第六医院（丽水市人民医院）
通讯作者	Jia, Chang
作者单位	1.The Institute of Life Sciences,Wenzhou University,Wenzhou,China; 2.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China; 3.Department of Orthopedics Surgery,Lishui People's Hospital,The Sixth Affiliated Hospital of Wenzhou Medical University,Lishui,Zhe Jiang,China; 4.Pediatric Research Institute,The Second Affiliated Hospital and Yuying Children’ s Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	附属第二医院
推荐引用方式 GB/T 7714	Xu, Ke,An, Ningchen,Zhang, Haijuan,et al. Sustained-release of PDGF from PLGA microsphere embedded thermosensitive hydrogel promoting wound healing by inhibiting autophagy[J]. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY,2020,55.
APA	Xu, Ke., An, Ningchen., Zhang, Haijuan., Zhang, Qilu., Zhang, Kailun., ... & Jia, Chang. (2020). Sustained-release of PDGF from PLGA microsphere embedded thermosensitive hydrogel promoting wound healing by inhibiting autophagy. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 55.
MLA	Xu, Ke,et al."Sustained-release of PDGF from PLGA microsphere embedded thermosensitive hydrogel promoting wound healing by inhibiting autophagy".JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 55(2020).