Building endoplasmic reticulum stress-related LncRNAs signatures of lung adenocarcinoma

doi:10.1002/jgm.3731

科研成果详情

题名	Building endoplasmic reticulum stress-related LncRNAs signatures of lung adenocarcinoma
作者	Chen, Kai1; Dai, Peiling2; Gu, Lizhong1
发表日期	2024-08
发表期刊	JOURNAL OF GENE MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	endoplasmic reticulum stress LncRNAs lung adenocarcinoma signature
其他关键词	LONG NONCODING RNA ; CANCER STATISTICS ; PROLIFERATION ; APOPTOSIS ; MIGRATION ; BIOMARKER ; SNHG12
摘要	BackgroundEndoplasmic reticulum stress (ERS) could be a strategy for treating malignant tumors. Moreover, long noncoding RNAs (lncRNAs) can promote tumorigenesis and progression, and forecast the prognosis of cancers. Nevertheless, the prognostic value of ERS-related lncRNAs has not been reported in lung adenocarcinoma (LUAD).MethodsThe messenger RNA (mRNA), microRNA (miRNA) and lncRNA expression data related to LUAD were obtained in public databases (TCGA and GEO databases). Prognostic ERS-related differentially expressed lncRNAs (ERS-DELs) were obtained and used to build an ERS-related model by Cox regression analysis. Moreover, we further screened independent prognostic elements and built a nomogram. Furthermore, enrichment analysis of genes was conducted to investigate the functions. A lncRNA-miRNA-mRNA network was built to explore mechanism of lncRNAs. Finally, qRT-PCR was utilized to examine the expression levels of lncRNAs.Results30 ERS-DELs were identified, and an ERS-related signature was built based on AF131215.2, LINC00472, LINC01352, RP1-78O14.1, RP11-253E3.3, RP11-98D18.9, and SNHG12. Gene set enrichment analysis indicated that genes in the high-risk group were chiefly focused on the regulation of mRNA binding, and genes in the low-risk group were significantly focused on protein localization to cilia. A lncRNA-miRNA-mRNA network, containing 7 signature lncRNAs, 23 miRNAs, and 128 mRNAs, was also established. Eventually, quantitative real-time polymerase chain reaction was used to confirm that seven prognostic lncRNAs had a consistent expression with the analysis.ConclusionsAn ERS-related signature containing seven prognostic lncRNAs was built, which offered new thinking concerning the role of ERS-related lncRNAs in LUAD. This study identifies a prognostic signature for lung adenocarcinoma (LUAD) based on endoplasmic reticulum stress-related long noncoding RNAs (ERSLs). Thirty ERS-related differentially expressed lncRNAs (ERS-DELs) were identified, and a model including seven lncRNAs (AF131215.2, LINC00472, LINC01352, RP1-78O14.1, RP11-253E3.3, RP11-98D18.9, and SNHG12) was developed. Gene set enrichment analysis revealed distinct functional pathways in high- and low-risk groups. A lncRNA-miRNA-mRNA network was constructed, and qRT-PCR validated the expression of the seven lncRNAs, offering new insights into ERSLs' role in LUAD prognosis. image
出版者	WILEY
ISSN	1099-498X
EISSN	1521-2254
卷号	26 期号:8
DOI	10.1002/jgm.3731
页数	15
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
WOS记录号	WOS:001290835500001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39146558
SCOPUSEID	2-s2.0-85201146282
通讯作者地址	[Gu, Lizhong]Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiovasc & Thorac Surg, 109 Xueyuan Rd, Wenzhou 325027, Peoples R China.
Scopus学科分类	Molecular Medicine;Molecular Biology;Genetics;Drug Discovery;Genetics (clinical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217493
专题	附属第二医院附属第一医院附属第一医院_放化疗科附属第二医院_心胸外科第二临床医学院、附属第二医院、育英儿童医院附属第二医院_血管外科
通讯作者	Gu, Lizhong
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiovasc & Thorac Surg, 109 Xueyuan Rd, Wenzhou 325027, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1, Dept Radiotherapy, Wenzhou, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 血管外科; 心胸外科
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 血管外科; 心胸外科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Chen, Kai,Dai, Peiling,Gu, Lizhong. Building endoplasmic reticulum stress-related LncRNAs signatures of lung adenocarcinoma[J]. JOURNAL OF GENE MEDICINE,2024,26(8).
APA	Chen, Kai, Dai, Peiling, & Gu, Lizhong. (2024). Building endoplasmic reticulum stress-related LncRNAs signatures of lung adenocarcinoma. JOURNAL OF GENE MEDICINE, 26(8).
MLA	Chen, Kai,et al."Building endoplasmic reticulum stress-related LncRNAs signatures of lung adenocarcinoma".JOURNAL OF GENE MEDICINE 26.8(2024).