Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury

doi:10.1016/j.intimp.2024.112921

科研成果详情

题名	Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury
作者	Huang, Kaiyu 1,2; Cai, Chenchen2,3; He, Hualing1,2; Yi, Binghua1,2; Xu, Wencai1,2; Lin, Zhonghao 1,2; Lv, Xiao1,2; Liu, Ronghua1,2; Zheng, Cheng1,2; Zhou, Yingying 2,4; Lin, Jiafeng1,2
发表日期	2024-10-25
发表期刊	International Immunopharmacology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Corylin Myocardial ischemia-reperfusion Raf-1/ASK1 complex Apoptosis
其他关键词	SIGNAL-REGULATING KINASE-1 ; PATHWAY
摘要	Effective treatment of myocardial ischemia-reperfusion (MIR) injury remains an unmet clinical need. Cardiomyocyte apoptosis is common at this stage and poses a significant risk. Corylin, a flavonoid compound extracted from Psoralea corylifolia L., has been shown to have anti-inflammatory, anticancer, and antiatherosclerotic properties. However, whether and how corylin affects MIR injury remain unclear. In this study, we explored the mechanism of corylin as a potent therapeutic agent for MI/R injury, using a left anterior descending (LAD) coronary artery ligation and oxygen-glucose deprivation and reperfusion (OGD/R) model in vivo and in vitro. TUNEL, Annexin-V/PI double staining,Ki67 immunohistochemistry, western blot analysis, and immunofluorescence were used to validate cell apoptosis level and Raf-1/ASK1 complex activity. The interaction between corylin and Raf-1/ASK1 complex was detected using molecular docking, corylin-Raf-1 binding assays, and coimmunoprecipitation (Co-IP). Moreover, TTC staining, echocardiography, HE staining, Masson trichrome staining and serological testing were performed to assess the cardioprotective effects of corylin in vivo. These findings showed that corylin reduces MIR injury-induced cardiomyocyte apoptosis and improves cardiac function. Mechanistically, corylin can interact with Raf-1 and promote the formation of the Raf-1/ASK1 complex, thus inhibiting cardiomyocyte apoptosis. In conclusion, our results demonstrate that corylin ameliorated cardiac dysfunction after MIR injury by reducing myocardial apoptosis.
资助项目	Zhejiang Provincial Natural Science Foundation of China [LQ22H020001, LY21H020011]; Na-tional Natural Science Foundation of China [82070333]; Wenzhou Science and Technology Bureau Major Scientific Research Project [ZY2020018]; Scientific Research Foundation of the Science and Technology Department of Wenzhou City [Y20210191, Y20210140, Y20220472, Y2020064]
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	140
DOI	10.1016/j.intimp.2024.112921
页数	10
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:001294943100001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39133953
SCOPUSEID	2-s2.0-85200959206
通讯作者地址	[Lin, Jiafeng]Wenzhou Med Univ, Yuying Childrens Hosp, Xueyuanxi Rd 109, Wenzhou 325000, Zhejiang, Peoples R China. ; [Lin, Jiafeng]Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiol, Xueyuanxi Rd 109, Wenzhou 325000, Zhejiang, Peoples R China.
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
SCOPUS_ID	SCOPUS_ID:85200959206
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217480
专题	附属第二医院附属第二医院_耳鼻咽喉科附属第二医院_内分泌科第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Lin, Jiafeng
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Cardiol, Key Lab Panvasc Dis Wenzhou, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Xueyuanxi Rd 109, Wenzhou 325000, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp 2, Dept Phys Med & Rehabil, Wenzhou, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 2, Dept Endocrinol, Wenzhou, Zhejiang, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 耳鼻咽喉科
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 耳鼻咽喉科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Huang, Kaiyu,Cai, Chenchen,He, Hualing,et al. Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury[J]. International Immunopharmacology,2024,140.
APA	Huang, Kaiyu., Cai, Chenchen., He, Hualing., Yi, Binghua., Xu, Wencai., ... & Lin, Jiafeng. (2024). Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury. International Immunopharmacology, 140.
MLA	Huang, Kaiyu,et al."Promotion of Raf-1/ASK1 complex formation by corylin inhibits cell apoptosis in myocardial ischemia/reperfusion injury".International Immunopharmacology 140(2024).