科研成果详情

题名Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer
作者
发表日期2024-08-30
发表期刊HELIYON   影响因子和分区
语种英语
原始文献类型Article
关键词Exosomes Gemcitabine PPP3CB Pancreatic cancer miR-298/STAT3
其他关键词COLORECTAL-CANCER ; CHEMORESISTANCE ; PROLIFERATION ; EPIDEMIOLOGY ; APOPTOSIS ; CELLS
摘要Purpose: Due to resistance to gemcitabine (GEM), patients with pancreatic cancer (PC) usually have poor prognosis and low survival rate. The purpose of our research was to explore the impact of exosome PPP3CB on GEM resistance in PC, and concurrently analyze the regulatory role of the miR-298/STAT3 signaling pathway. Methods: Exosomes isolated from PC cells were verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). The interaction between PPP3CB and miR-298 was verified using dual-luciferase reporter gene assay, followed by evaluation of cell growth and death using CCK8 assay, EdU staining, and flow cytometry. Results: Increased PPP3CB expression was observed in GEM-resistant PC cells. Exosomes from PC cells and GEM-resistant PC cells were successfully extracted by ultra-high speed centrifugation. Confocal microscopy showed internalization of fluorescein amide (FAM)-labeled GEM-resistant exosomes by PC cells. PPP3CB enhanced the proliferation of GEM-resistant PC cells and inhibited their apoptosis, whereas down-regulation of PPP3CB promoted the death of PC cells and inhibited the proliferation of GEM-resistant PC cells, and enhance the susceptibility of PC cells to GEM. Additionally, PPP3CB positively regulated STAT3 expression in PC cells by down-regulating miR-298, thus promoting the growth and inhibiting the death of PC cells. Conclusion: PC cell-derived exosome PPP3CB enhances STAT3 expression by downregulating miR298, stimulating cell growth, and suppressing cell death, thereby increasing the resistance of PC cells to GEM.
资助项目Zhejiang Basic Public Welfare Research Project [LGF22H160059]
出版者CELL PRESS
ISSN2405-8440
EISSN2405-8440
卷号10期号:16
DOI10.1016/j.heliyon.2024.e36434
页数12
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
WOS记录号WOS:001297799600001
收录类别SCIE ; SCOPUS
URL查看原文
PubMed ID39253142
SCOPUSEID2-s2.0-85201477677
通讯作者地址[Qin, Yong]Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Hepatobiliary Pancreat Surg,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China.
Scopus学科分类Multidisciplinary
引用统计
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/217467
专题附属第一医院
其他_附属第六医院(丽水市人民医院)
通讯作者Qin, Yong
作者单位
1.Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Ultrasound,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China;
2.Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Hepatobiliary Pancreat Surg,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China
第一作者单位附属第一医院;  附属第六医院(丽水市人民医院)
通讯作者单位附属第一医院;  附属第六医院(丽水市人民医院)
第一作者的第一单位附属第一医院
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Wang, Chaojun,Xu, Shengqian,Qin, Yong. Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer[J]. HELIYON,2024,10(16).
APA Wang, Chaojun, Xu, Shengqian, & Qin, Yong. (2024). Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer. HELIYON, 10(16).
MLA Wang, Chaojun,et al."Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer".HELIYON 10.16(2024).

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