题名 | Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer |
作者 | |
发表日期 | 2024-08-30 |
发表期刊 | HELIYON 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Exosomes Gemcitabine PPP3CB Pancreatic cancer miR-298/STAT3 |
其他关键词 | COLORECTAL-CANCER ; CHEMORESISTANCE ; PROLIFERATION ; EPIDEMIOLOGY ; APOPTOSIS ; CELLS |
摘要 | Purpose: Due to resistance to gemcitabine (GEM), patients with pancreatic cancer (PC) usually have poor prognosis and low survival rate. The purpose of our research was to explore the impact of exosome PPP3CB on GEM resistance in PC, and concurrently analyze the regulatory role of the miR-298/STAT3 signaling pathway. Methods: Exosomes isolated from PC cells were verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). The interaction between PPP3CB and miR-298 was verified using dual-luciferase reporter gene assay, followed by evaluation of cell growth and death using CCK8 assay, EdU staining, and flow cytometry. Results: Increased PPP3CB expression was observed in GEM-resistant PC cells. Exosomes from PC cells and GEM-resistant PC cells were successfully extracted by ultra-high speed centrifugation. Confocal microscopy showed internalization of fluorescein amide (FAM)-labeled GEM-resistant exosomes by PC cells. PPP3CB enhanced the proliferation of GEM-resistant PC cells and inhibited their apoptosis, whereas down-regulation of PPP3CB promoted the death of PC cells and inhibited the proliferation of GEM-resistant PC cells, and enhance the susceptibility of PC cells to GEM. Additionally, PPP3CB positively regulated STAT3 expression in PC cells by down-regulating miR-298, thus promoting the growth and inhibiting the death of PC cells. Conclusion: PC cell-derived exosome PPP3CB enhances STAT3 expression by downregulating miR298, stimulating cell growth, and suppressing cell death, thereby increasing the resistance of PC cells to GEM. |
资助项目 | Zhejiang Basic Public Welfare Research Project [LGF22H160059] |
出版者 | CELL PRESS |
ISSN | 2405-8440 |
EISSN | 2405-8440 |
卷号 | 10期号:16 |
DOI | 10.1016/j.heliyon.2024.e36434 |
页数 | 12 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
WOS记录号 | WOS:001297799600001 |
收录类别 | SCIE ; SCOPUS |
URL | 查看原文 |
PubMed ID | 39253142 |
SCOPUSEID | 2-s2.0-85201477677 |
通讯作者地址 | [Qin, Yong]Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Hepatobiliary Pancreat Surg,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China. |
Scopus学科分类 | Multidisciplinary |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/217467 |
专题 | 附属第一医院 其他_附属第六医院(丽水市人民医院) |
通讯作者 | Qin, Yong |
作者单位 | 1.Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Ultrasound,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1,Lishui Univ, Lishui Peoples Hosp, Dept Hepatobiliary Pancreat Surg,Lishui Hosp, Lishui 323000, Zhejiang, Peoples R China |
第一作者单位 | 附属第一医院; 附属第六医院(丽水市人民医院) |
通讯作者单位 | 附属第一医院; 附属第六医院(丽水市人民医院) |
第一作者的第一单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Wang, Chaojun,Xu, Shengqian,Qin, Yong. Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer[J]. HELIYON,2024,10(16). |
APA | Wang, Chaojun, Xu, Shengqian, & Qin, Yong. (2024). Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer. HELIYON, 10(16). |
MLA | Wang, Chaojun,et al."Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer".HELIYON 10.16(2024). |
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