Strontium zinc silicate simultaneously alleviates osteoporosis and sarcopenia in tail-suspended rats via Piezo1-mediated Ca2+ signaling

doi:10.1016/j.jot.2024.07.014

科研成果详情

题名	Strontium zinc silicate simultaneously alleviates osteoporosis and sarcopenia in tail-suspended rats via Piezo1-mediated Ca2+ signaling
作者	Huang, Lingwei 1,2,3; Jiao, Yiren 1,2; Xia, Hangbin1,2,4; Li, Huili1,2,4; Yu, Jing1,2; Que, Yumei1,2; Zeng, Zhen 1,2,3; Fan, Chen 1,2; Wang, Chen 2; Yang, Chen 1,2; Chang, Jiang 1,2,5
发表日期	2024-09
发表期刊	Journal of Orthopaedic Translation 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Intracellular Ca 2 + Myogenesis Osteogenesis Osteosarcopenia Piezo1 Strontium zinc silicate
其他关键词	ELECTRICAL-STIMULATION ; BONE LOSS ; MUSCLE ; EXERCISE ; DIFFERENTIATION ; TIBIA ; SI
摘要	Background: Long-term physical inactivity probably leads to a co-existence of osteoporosis and sarcopenia which result in a high risk of falls, fractures, disability and even mortality. However, universally applicable and feasible approaches are lacking in the concurrent treatment of osteoporosis and sarcopenia. In this study, we evaluated the effect of strontium zinc silicate bioceramic (SZS) extract on osteoporosis and sarcopenia and explored its underlying mechanisms. Methods: Hindlimb osteoporosis and sarcopenia were established in a tail-suspended rat model. The bones were conducted mu CT scanning, histological examination, and gene expression analysis, and the muscles were conducted histological examination and gene expression analysis. In vitro, the effect of SZS extract on osteoblasts was determined by alizarin red S staining, immunofluorescence and qPCR. Similarly, the effect of SZS extract on myoblasts was determined by immunofluorescence and qPCR.. At last, the role of Piezo1 and the change of intracellular calcium ion (Ca2+) were explored through blockading the Piezo1 by GsMTx4 in MC3T3-E1 and C2C12 cells, respectively. Results: We found that SZS extract could concurrently and efficiently prevent bone structure deterioration, muscle atrophy and fibrosis in hind limbs of the tail-suspended rats. The in vivo study also showed that SZS extract could upregulate the mRNA expression of Piezo1, thereby maintaining the homeostasis of bones and muscles. In vitro study demonstrated that SZS extract could promote the proliferation and differentiation of MC3T3-E1 and C2C12 cells by increasing the intracellular Ca2+ in a Piezo1-dependent manner. Conclusion: This study demonstrated that SZS extract could increase Piezo1-mediated intracellular Ca2+, and facilitate osteogenic differentiation of osteoblast and myogenic differentiation of myoblasts, contributing to alleviation of osteoporosis and sarcopenia in a tail-suspended rat model. The translational potential of this article: The current study might provide a universally applicable and efficient strategy to treat musculoskeletal disorders based on bioactive ceramics. The verification of the role of Piezo1modulated intracellular Ca2+ during osteogenesis and myogenesis provided a possible therapeutic target against mechanical related diseases.
资助项目	National Key Research and Development Program of China [2023YFB3810202]; National Natural Science Foundation of China [32271386]; Opening Research fund from Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine [2022SKLS-KFKT005]; Wenzhou Basic Scientific Research Project [Y2023068]; Wenzhou Institute, University of Chinese Academy of Sciences [WIUCASQD2020013, WIUCASQD2021030, WIUCASQD2021029, WIUCASQD2022032, WIUCASQD2023019]; First Affiliated Hospital of Wenzhou Medical University
出版者	ELSEVIER
ISSN	2214-031X
卷号	48 页码:146-155
DOI	10.1016/j.jot.2024.07.014
页数	10
WOS类目	Orthopedics
WOS研究方向	Orthopedics
WOS记录号	WOS:001295744500001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39229332
SCOPUSEID	2-s2.0-85201073279
通讯作者地址	[Yang, Chen;Chang, Jiang]Wenzhou Med Univ, Affiliated Hosp 1, Joint Ctr Translat Med, Wenzhou 325000, Peoples R China.
Scopus学科分类	Orthopedics and Sports Medicine
SCOPUS_ID	SCOPUS_ID:85201073279
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217352
专题	附属第一医院眼视光学院（生物医学工程学院）、附属眼视光医院眼视光学院（生物医学工程学院）、附属眼视光医院_医学工程系
通讯作者	Yang, Chen; Chang, Jiang
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Joint Ctr Translat Med, Wenzhou 325000, Peoples R China; 2.Univ Chinese Acad Sci, Wenzhou Inst, Zhejiang Engn Res Ctr Tissue Repair Mat, Wenzhou 325000, Peoples R China; 3.Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China; 4.Wenzhou Med Univ, Sch Ophthalmol & Optometry, Sch Biomed Engn, Wenzhou 325027, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, Lingwei,Jiao, Yiren,Xia, Hangbin,et al. Strontium zinc silicate simultaneously alleviates osteoporosis and sarcopenia in tail-suspended rats via Piezo1-mediated Ca2+ signaling[J]. Journal of Orthopaedic Translation,2024,48:146-155.
APA	Huang, Lingwei., Jiao, Yiren., Xia, Hangbin., Li, Huili., Yu, Jing., ... & Chang, Jiang. (2024). Strontium zinc silicate simultaneously alleviates osteoporosis and sarcopenia in tail-suspended rats via Piezo1-mediated Ca2+ signaling. Journal of Orthopaedic Translation, 48, 146-155.
MLA	Huang, Lingwei,et al."Strontium zinc silicate simultaneously alleviates osteoporosis and sarcopenia in tail-suspended rats via Piezo1-mediated Ca2+ signaling".Journal of Orthopaedic Translation 48(2024):146-155.