橙皮苷促进小鼠急性皮肤创面愈合的作用研究

doi:10.13699/j.cnki.1001-6821.2024.14.019

科研成果详情

题名	橙皮苷促进小鼠急性皮肤创面愈合的作用研究
其他题名	Effects of hesperidin in promoting acute skin wound healing
作者	黄乙明1; 陈飞飞 2; 张蓉蓉 1; 朱光辉1
发表日期	2024-07-19
发表期刊	中国临床药理学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	橙皮苷创面愈合 Wnt/β-catenin 人角质形成细胞人皮肤成纤维细胞
其他关键词	hesperidin ; wound healing ; Wnt/β-catenin ; human keratinocytes ; human skin fibroblasts
摘要	目的研究橙皮苷对人角质形成细胞（HaCaT）和人皮肤成纤维细胞（HSF）迁移能力的影响，初步探索橙皮苷促进小鼠急性皮肤创面愈合的作用及相关机制。方法将HaCaT和HSF分为空白组（不做任何处理）、对照组（加入0.1%二甲基亚砜）和实验组(加入5.0μg·mL-1的橙皮苷),培养48 h。用细胞划痕实验检测细胞体外愈合能力，用Transwell迁移实验检测细胞迁移情况。将C57小鼠随机分为模型组和低、高剂量实验组，用手术剪剪下小鼠背部全层皮肤建立急性皮肤全层缺损创面模型。模型组给予0.5%二甲基亚砜，低、高剂量实验组分别给予10、50 mg·kg-1 橙皮苷溶液。用蛋白质印迹法检测β-连环蛋白、增殖细胞核抗原（PCNA）、角蛋白14、Ⅰ型胶原蛋白的表达情况。结果 HaCaT-空白组、HaCaT-对照组和HaCaT-实验组的划痕愈合率分别为（21.05±1.10）%、（22.33±1.72）%、（41.61±2.90）%,细胞迁移数分别为（57.00±11.36）、（60.38±10.11）、（287.75±20.21）个；HSF-空白组、HSF-对照组和HSF-实验组的划痕愈合率分别为（17.82±1.62）%、（19.81±3.87）%、（64.22±1.94）%,细胞迁移数分别为（43.25±7.98）、（40.75±6.70）、（140.88±14.35）个。HaCaT-实验组的上述指标与HaCaT-空白组、HaCaT-对照组比较，HSF-实验组的上述指标与HSF-空白组、HSF-对照组比较，在统计学上差异均有统计学意义（均P<0.05）。模型组和低、高剂量实验组β-连环蛋白表达水平分别为0.53±0.06、0.74±0.17和1.44±0.11,角蛋白14相对表达水平分别为0.33±0.06、0.54±0.07和1.26±0.16,PCNA相对表达水平分别为0.46±0.05、0.72±0.09和1.14±0.11,Ⅰ型胶原蛋白相对表达水平分别为0.52±0.03、0.77±0.05和1.28±0.13。低、高剂量实验组的上述指标与模型组相比，在统计学上差异均有统计学意义（P<0.05,P<0.01）。结论橙皮苷可能通过激活Wnt/β-catenin信号通路，增加HaCaT和HSF的迁移，促进小鼠急性皮肤创面愈合。
其他摘要	Objective To study the effects of hesperidin on the migration ability of human keratinocytes(HaCaT)and human skin fibroblasts(HSF).Additionally,this research aims to preliminary investigate the influence and underlying mechanism of Hesperidin in facilitating the healing process of acute skin wounds in mice.Methods HaCaT and HSF were divided into blank group(without any treatment),control group(added 0.1％dimethyl sulfoxide)and experimental group(added 5.0 μg·mL-1 hesperidin)for 48 h.The healing ability of cells in vitro was detected by scratch test.The migration of cells was detected by Transwell migration test.C57 mice were randomly divided into model group,experimental-L,-H groups.The acute full-thickness skin defect wound model was established by surgical clipping of the full-thickness skin of the back of mice.The model group was given 0.5％dimethyl sulfoxide,and the experimental-L,-H groups were given 10,50 mg·kg-1 hesperidin solution,respectively.The protein expressions levels of β-catenin,proliferating cell nuclear antigen(PCNA),keratin 14 and collagen Ⅰ were detected by Western blot.Results The scratch healing rates of HaCaT-blank group,HaCaT-control group and HaCaT-experimental group were(21.05±1.10)％,(22.33±1.72)％and(41.61±2.90)％;the cell migration numbers were 57.00±11.36,60.38±10.11 and 287.75±20.21,respectively.The scratch healing rates of HSF-blank group,HSF-control group and HSF-experimental group were(17.82±1.62)％,(19.81±3.87)％and(64.22±1.94)％,the cell migration numbers were 43.25±7.98,40.75±6.70 and 140.88±14.35,respectively.The HaCaT-experimental group was compared with HaCaT-blank group and HaCaT-control group,and the HSF-experimental group was compared with HSF-blank group and HSF-control group,the differences were statistically significant(all P＜0.05).The protein expression levels of β-catenin in the model group,experimental-L,-H groups were 0.53±0.06,0.74±0.17 and 1.44±0.11;the protein expression levels of keratin 14 were 0.33±0.06,0.54±0.07 and 1.26±0.16;the protein expression levels of PCNA were 0.46±0.05,0.72±0.09 and 1.14±0.11;the protein levels of collagen Ⅰ were 0.52±0.03,0.77±0.05 and 1.28±0.13,respectively.There were significant differences in the above indexes between the experimental-L,-H groups and the model group(P＜0.05,P＜0.01).Conclusion Hesperidin may promote the healing of acute skin wounds in mice by activating the Wnt/β-catenin signaling pathway and increasing the migration of HaCaT and HSF.
ISSN	1001-6821
卷号	40 期号:14 页码:2093-2097
DOI	10.13699/j.cnki.1001-6821.2024.14.019
页数	5
收录类别	CNKI ; 万方 ; 维普 ; 北大核心 ; ISTIC ; CSCD
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217174
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院眼视光学院（生物医学工程学院）、附属眼视光医院_药剂科
通讯作者	朱光辉
作者单位	1.温州医科大学附属第二医院,育英儿童医院药学部; 2.温州医科大学附属台州妇女儿童医院药剂科
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	黄乙明,陈飞飞,张蓉蓉,等. 橙皮苷促进小鼠急性皮肤创面愈合的作用研究[J]. 中国临床药理学杂志,2024,40(14):2093-2097.
APA	黄乙明, 陈飞飞, 张蓉蓉, & 朱光辉. (2024). 橙皮苷促进小鼠急性皮肤创面愈合的作用研究. 中国临床药理学杂志, 40(14), 2093-2097.
MLA	黄乙明,et al."橙皮苷促进小鼠急性皮肤创面愈合的作用研究".中国临床药理学杂志 40.14(2024):2093-2097.