ZNF300 promotes proliferation and migration of hepatocellular carcinoma by upregulating c-MYC gene expression

doi:10.1016/j.clinre.2024.102415

科研成果详情

题名	ZNF300 promotes proliferation and migration of hepatocellular carcinoma by upregulating c-MYC gene expression
作者	Xiang, Wei; Ni, Junwei; Dong, Liyang; Zhu, Guoqing
发表日期	2024-08
发表期刊	CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Hepatocellular carcinoma ZNF300 c-MYC MAPK/ERK
其他关键词	SIGNALING PATHWAY ; MAPK/ERK
摘要	Background: Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver. Currently, the treatments of HCC are limited to surgical resection and liver transplantation, and there is no effective systemic therapy. Objectives: To investigate the regulatory mechanism of zinc finger protein 300 (ZNF300) in hepatocellular carcinoma (HCC). Methods: The expressions of ZNF300 in HCC tissue samples and HCC cell lines (Hep3B, Huh7, SNU-387) were detected. ZNF300 overexpression vector (ZNF300) or shRNAZNF300 (shZNF300) was transfected into HCC cells to increase or inhibit ZNF300 expression. 5-Ethynyl-2 '-deoxyuridine assay (EdU), cell counting kit-8 assay (CCK8) and transwell invasion assay were conducted to evaluate the proliferation, viability, migration, and invasion of HCC cells respectively. The expressions of tumor migration and invasion related proteins (matrix metallopeptidase 2 (MMP-2) and MMP-9), c-MYC, and MAPK/ERK signaling pathway related molecules (p-ERK1/2, ERK1/2, p-P38, P38) were determined by western blotting. Hep3B cells transfected with shZNF300 were subcutaneously injected into nude mice to perform tumor xenograft experiment. Tumor volume and weight were measured. Results: ZNF300 was upregulated in HCC tissues and cells. The expressions of MMP-2 and MMP-9 were increased in HCC cells after transfecting with ZNF300 but reduced in HCC cells transfected with shZNF300. Downregulation of ZNF300 inhibited HCC cell proliferation, migration, and invasion, while overexpression of ZNF300 showed the opposite effects. Moreover, the expressions of c-MYC and MAPK/ERK signaling pathway related molecules were increased after overexpression of ZNF300 but reduced after downregulating ZNF300. In tumor xenograft experiment, downregulation of ZNF300 reduced tumor volume and weight. Conclusion: The present study proved that downregulation of ZNF300 inhibited HCC growth by reducing c-MYC expression and MAPK/ERK signaling pathway.
出版者	ELSEVIER MASSON, CORP OFF
ISSN	2210-7401
EISSN	2210-741X
卷号	48 期号:7
DOI	10.1016/j.clinre.2024.102415
页数	7
WOS类目	Gastroenterology & Hepatology
WOS研究方向	Gastroenterology & Hepatology
WOS记录号	WOS:001273344900001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39018766
SCOPUSEID	2-s2.0-85198513906
通讯作者地址	[Ni, Junwei]Wenzhou Med Univ, Dept Intervent, Affiliated Hosp 1, Nanbaixiang St, Wenzhou 325015, Zhejiang, Peoples R China.
Scopus学科分类	Gastroenterology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216882
专题	附属第一医院附属第一医院_介入科
通讯作者	Ni, Junwei
作者单位	Wenzhou Med Univ, Dept Intervent, Affiliated Hosp 1, Nanbaixiang St, Wenzhou 325015, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 介入科
通讯作者单位	附属第一医院; 介入科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Xiang, Wei,Ni, Junwei,Dong, Liyang,et al. ZNF300 promotes proliferation and migration of hepatocellular carcinoma by upregulating c-MYC gene expression[J]. CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY,2024,48(7).
APA	Xiang, Wei, Ni, Junwei, Dong, Liyang, & Zhu, Guoqing. (2024). ZNF300 promotes proliferation and migration of hepatocellular carcinoma by upregulating c-MYC gene expression. CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY, 48(7).
MLA	Xiang, Wei,et al."ZNF300 promotes proliferation and migration of hepatocellular carcinoma by upregulating c-MYC gene expression".CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY 48.7(2024).