Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease

doi:10.1016/j.ijcard.2024.132364

科研成果详情

题名	Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease
作者	Yu, Lili 1,2,3,4,5; Li, Yucui1,2,3,6; Zhang, Yingying1,2,3,6; Weng, Luyi1,2,3; Shuai, Dujuan1,2,3,6; Zhu, Jinshun1,2,3,6; Niu, Chao1,2,3,6; Chu, Maoping1,2,3,6; Jia, Chang1,2,3,6
发表日期	2024-10-15
发表期刊	International Journal of Cardiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Kawasaki disease HCMV pUL135 Endothelial dysfunction CD2AP
其他关键词	ACTIN CYTOSKELETON ; CAPPING PROTEIN ; PROLIFERATION ; DYNAMICS ; REMODELS ; GROWTH ; LINK
摘要	Background: Kawasaki disease (KD) is a kind of pediatric vasculitis, whose pathogenesis has not been elucidated until now. Many scholars believe that KD is one type of infectious diseases in the susceptible groups. However, no recognized pathogens are confirmed. Human cytomegalovirus (HCMV) is a ubiquitous human herpes virus, which can infect varieties of cells including endothelial cells. Studies reported that the viral protein pUL135 is very important for virus replication, reactivation and immune escape. Therefore, we hypothesize that HCMV pUL135 may have a pathogenic effect on KD. Methods: We first determined pUL135 levels in the serum from KD patients. Next, we examined the effects and mechanisms of pUL135 on endothelial cell proliferation and migration. Finally, we assessed the effect of pUL135 on cardiac inflammation in a KD murine model. Results: Data showed that pUL135 level was significantly increased in the serum from KD patients compared with the healthy and fever controls. And pUL135 expression in endothelial cells remarkably inhibited cell proliferation, migration and tube formation. Moreover, expression of pUL135 obviously affected actin cytoskeleton. Mechanism investigation substantiated that pUL135 mediated endothelial cell dysfunction via regulating CD2AP. Ultimately, we found that HCMV pUL135 aggravated coronary arteritis in the Candida albicans cell wall extracts (CAWS)-induced KD mouse model. Conclusion: Our findings imply that HCMV pUL135-mediated endothelial dysfunction plays an important role in exacerbating coronary artery injury in KD conditions.
资助项目	National Natural Science Foun-dation of China [82370509, 82370510, 81970435, 82000469, 82100523]; Zhejiang Provincial Science and Technology Project of Traditional Chinese Medicine [2024ZL614]
出版者	ELSEVIER IRELAND LTD
ISSN	0167-5273
EISSN	1874-1754
卷号	413
DOI	10.1016/j.ijcard.2024.132364
页数	10
WOS类目	Cardiac & Cardiovascular Systems
WOS研究方向	Cardiovascular System & Cardiology
WOS记录号	WOS:001276416900001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39025135
SCOPUSEID	2-s2.0-85198918593
通讯作者地址	[Chu, Maoping;Jia, Chang]Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China. ; [Chu, Maoping;Jia, Chang]Wenzhou Med Univ, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China.
Scopus学科分类	Cardiology and Cardiovascular Medicine
SCOPUS_ID	SCOPUS_ID:85198918593
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216833
专题	附属第二医院附属第二医院_儿童心血管科第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Chu, Maoping; Jia, Chang
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Pediat Res Inst, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China; 3.Key Lab Struct Malformat Childern Zhejiang Prov, Wenzhou 325027, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Yuying Childrens Hosp, Dept Pediat, Wenzhou, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 2, Key Lab Children Genitourinary Dis Wenzhou, Wenzhou, Zhejiang, Peoples R China; 6.Wenzhou Med Univ, Affiliated Hosp 2, Childrens Heart Ctr, Wenzhou 325027, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Yu, Lili,Li, Yucui,Zhang, Yingying,et al. Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease[J]. International Journal of Cardiology,2024,413.
APA	Yu, Lili., Li, Yucui., Zhang, Yingying., Weng, Luyi., Shuai, Dujuan., ... & Jia, Chang. (2024). Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease. International Journal of Cardiology, 413.
MLA	Yu, Lili,et al."Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease".International Journal of Cardiology 413(2024).