Establishment of a multi-line immunochromatography based on magnetic nanoparticles for simultaneous screening of multiple biomarkers

doi:10.1007/s00216-024-05432-4

科研成果详情

题名	Establishment of a multi-line immunochromatography based on magnetic nanoparticles for simultaneous screening of multiple biomarkers
作者	Shen, Liyue 1,2; Peng, Tao 2; Dong, Jiahui 1,2; Liang, Zhanwei 2; Si, Jihao 2; Ye, Hua3; Xie, Jie 2; Yu, Xiaoping 1; Dai, Xinhua 2
发表日期	2024-07-10
发表期刊	Analytical and Bioanalytical Chemistry 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	Magnetic nanoparticles Immunochromatography Liver cancer biomarkers Multiplex detection
其他关键词	TUMOR-MARKERS ; HEPATOCELLULAR-CARCINOMA ; QUANTITATIVE DETECTION ; SENSITIVE DETECTION ; EARLY-DIAGNOSIS ; TEST STRIP ; PIVKA-II ; ASSAY ; CLENBUTEROL ; PROTEIN
摘要	Biomarkers screening is a benefit approach for early diagnosis of major diseases. In this study, magnetic nanoparticles (MNPs) have been utilized as labels to establish a multi-line immunochromatography (MNP-MLIC) for simultaneous detection of carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA 19-9), and alpha-fetoprotein (AFP) in a single serum sample. Under the optimal parameters, the three biomarkers can be rapidly and simultaneously qualitative screening within 15 min by naked eye. As for quantitative detection, the MNP-MLIC test strips were precisely positioned and captured by a smartphone, and signals on the test and control lines were extracted by ImageJ software. The signal ratio of test and control lines has been calculated and used to plot quantitative standard curves with the logarithmic concentration, of which the correlation coefficients are more than 0.99, and the limit of detection for CEA, CA 19-9, and AFP were 0.60 ng/mL, 1.21 U/mL, and 0.93 ng/mL, respectively. The recoveries of blank serum were 75.0 similar to 112.5% with the relative standard deviation ranging from 2.5 to 15.3%, and the specificity investigation demonstrated that the MNP-MLIC is highly specific to the three biomarkers. In conclusion, the developed MNP-MLIC offers a rapid, simple, accurate, and highly specific method for simultaneously detecting multiple biomarkers in serum samples, which provides an efficient and accurate approach for the early diagnosis of diseases.
资助项目	National Key Research and Development program of China [2022YFF0608402]; Plan for Leading Talents of Science and Technology Innovation [WR2202]
出版者	SPRINGER HEIDELBERG
ISSN	1618-2642
EISSN	1618-2650
卷号	416 期号:22 页码:4823-4831
DOI	10.1007/s00216-024-05432-4
页数	9
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:001268902900002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	38981912
SCOPUSEID	2-s2.0-85198057336
通讯作者地址	[Yu, Xiaoping]China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China. ; [Dai, Xinhua]Natl Inst Metrol, Technol Innovat Ctr Mass Spectrometry State Market, Ctr Adv Measurement Sci, Beijing 100029, Peoples R China.
Scopus学科分类	Analytical Chemistry;Biochemistry
SCOPUS_ID	SCOPUS_ID:85198057336
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216735
专题	其他_温州市第三临床学院（温州市人民医院）
通讯作者	Yu, Xiaoping; Dai, Xinhua
作者单位	1.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China; 2.Natl Inst Metrol, Technol Innovat Ctr Mass Spectrometry State Market, Ctr Adv Measurement Sci, Beijing 100029, Peoples R China; 3.Wenzhou Med Univ, Shanghai Univ, Affiliated Hosp 3, Wenzhou Peoples Hosp,Wenzhou Clin Inst 3,Dept Neur, Wenzhou 325000, Peoples R China
推荐引用方式 GB/T 7714	Shen, Liyue,Peng, Tao,Dong, Jiahui,et al. Establishment of a multi-line immunochromatography based on magnetic nanoparticles for simultaneous screening of multiple biomarkers[J]. Analytical and Bioanalytical Chemistry,2024,416(22):4823-4831.
APA	Shen, Liyue., Peng, Tao., Dong, Jiahui., Liang, Zhanwei., Si, Jihao., ... & Dai, Xinhua. (2024). Establishment of a multi-line immunochromatography based on magnetic nanoparticles for simultaneous screening of multiple biomarkers. Analytical and Bioanalytical Chemistry, 416(22), 4823-4831.
MLA	Shen, Liyue,et al."Establishment of a multi-line immunochromatography based on magnetic nanoparticles for simultaneous screening of multiple biomarkers".Analytical and Bioanalytical Chemistry 416.22(2024):4823-4831.