科研成果详情

题名Antimicrobial peptide A20L: in vitro and in vivo antibacterial and antibiofilm activity against carbapenem-resistant Klebsiella pneumoniae
作者
发表日期2024-07-09
发表期刊Microbiology spectrum   影响因子和分区
语种英语
原始文献类型Journal Article
关键词A20L Klebsiella pneumoniae antibacterial antibiofilm antimicrobial peptide carbapenem-resistance
其他关键词GENERATION ; LENGTH
摘要Antimicrobial resistance has become a growing public health threat in recent years. Klebsiella pneumoniae is one of the priority pathogens listed by the World Health Organization. Antimicrobial peptides are considered promising alternatives to antibiotics due to their broad-spectrum antibacterial activity and low resistance. In this study, we investigated the antibacterial activity of antimicrobial peptide A20L against K. pneumoniae. In vitro antibacterial activity of A20L against K. pneumoniae was demonstrated by broth microdilution method. We confirmed the in vivo efficacy of A20L by Galleria mellonella infection model. In addition, we found that A20L also had certain antibiofilm activity by crystal violet staining. We also evaluated the safety and stability of A20L, and the results revealed that at a concentration of ≤128 µg/mL, A20L exhibited negligible toxicity to RAW264.7 cells and no substantial toxicity to G. mellonella. A20L was stable at different temperatures and with low concentration of serum [5% fetal bovine serum (FBS)]; however, Ca2+, Mg2+, and high serum concentrations reduced the antibacterial activity of A20L. Scanning electron microscope (SEM) and membrane permeability tests revealed that A20L may exhibit antibacterial action by damaging bacterial cell membranes and increasing the permeability of outer membrane. Taken together, our results suggest that A20L has significant development potential as a therapeutic antibiotic alternative, which provides ideas for the treatment of K. pneumoniae infection.}, {AbstractText=A20L showed antibacterial and anti-infective efficacy in vitro and in vivo against Klebsiella pneumoniae. It can have an antibacterial effect by disrupting the integrity of cell membranes. A20L displayed anti-biofilm and anti-inflammatory activity against carbapenem-resistant K. pneumoniae and certain application potential in vivo, which provides a new idea for the clinical treatment of biofilm-associated infections
资助项目Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province [2022E10022]
出版者AMER SOC MICROBIOLOGY
ISSN2165-0497
卷号12期号:8
DOI10.1128/spectrum.03979-23
页数14
WOS类目Microbiology
WOS研究方向Microbiology
WOS记录号WOS:001270914900001
收录类别PUBMED ; SCIE ; SCOPUS
URL查看原文
PubMed ID38980018
SCOPUSEID2-s2.0-85201029918
通讯作者地址[Zhou, Tieli;Ye, Jianzhong]First Affiliated Hosp Wenzhou Med Univ, Dept Clin Lab, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Wenzhou, Zhejiang, Peoples R China. ; [Zhou, Tieli;Ye, Jianzhong]Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Hangzhou, Peoples R China.
Scopus学科分类Physiology;Ecology;Immunology and Microbiology (all);Genetics;Microbiology (medical);Cell Biology;Infectious Diseases
引用统计
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/215740
专题附属第一医院
检验医学院(生命科学学院、生物学实验教学中心)
第一临床医学院(信息与工程学院)、附属第一医院
检验医学院(生命科学学院、生物学实验教学中心)_基础检验学系
第一临床医学院(信息与工程学院)、附属第一医院_临床检验诊断学
作者单位
1.Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, Wenzhou, Zhejiang, China.;
2.Department of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang, China.
第一作者单位附属第一医院;  临床检验诊断学;  第一临床医学院(信息与工程学院)、附属第一医院
通讯作者单位临床检验诊断学
第一作者的第一单位附属第一医院
推荐引用方式
GB/T 7714
Huijing Zhou,Xin Du,Yue Wang,et al. Antimicrobial peptide A20L: in vitro and in vivo antibacterial and antibiofilm activity against carbapenem-resistant Klebsiella pneumoniae[J]. Microbiology spectrum,2024,12(8).
APA Huijing Zhou., Xin Du., Yue Wang., Jingchun Kong., Xiaodong Zhang., ... & Jianzhong Ye. (2024). Antimicrobial peptide A20L: in vitro and in vivo antibacterial and antibiofilm activity against carbapenem-resistant Klebsiella pneumoniae. Microbiology spectrum, 12(8).
MLA Huijing Zhou,et al."Antimicrobial peptide A20L: in vitro and in vivo antibacterial and antibiofilm activity against carbapenem-resistant Klebsiella pneumoniae".Microbiology spectrum 12.8(2024).

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