Engineering of a DNA/γPNA Hybrid Nanoreporter for ctDNA Mutation Detection via γPNA Urinalysis

doi:10.1002/advs.202310225

科研成果详情

题名	Engineering of a DNA/γPNA Hybrid Nanoreporter for ctDNA Mutation Detection via γPNA Urinalysis
作者	Xiang, Zhichu1,2,3,4,5,6; Lu, Jianhua1,2; Ming, Yang 3,4,5,6; Guo, Weisheng 7,8; Chen, Xiaoyuan 3,4,5,6,9,10,11,12; Sun, Weijian1,2
发表日期	2024-07-03
发表期刊	Advanced Science 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	ctDNA mutation tumor detection urinalysis gamma PNA
其他关键词	CIRCULATING TUMOR DNA ; LIQUID BIOPSY ; NUCLEIC-ACIDS ; HYBRIDIZATION ; SENSORS ; PLASMA
摘要	Detection of circulating tumor DNA (ctDNA) mutations, which are molecular biomarkers present in bodily fluids of cancer patients, can be applied for tumor diagnosis and prognosis monitoring. However, current profiling of ctDNA mutations relies primarily on polymerase chain reaction (PCR) and DNA sequencing and these techniques require preanalytical processing of blood samples, which are time-consuming, expensive, and tedious procedures that increase the risk of sample contamination. To overcome these limitations, here the engineering of a DNA/gamma PNA (gamma peptide nucleic acid) hybrid nanoreporter is disclosed for ctDNA biosensing via in situ profiling and recording of tumor-specific DNA mutations. The low tolerance of gamma PNA to single mismatch in base pairing with DNA allows highly selective recognition and recording of ctDNA mutations in peripheral blood. Owing to their remarkable biostability, the detached gamma PNA strands triggered by mutant ctDNA will be enriched in kidneys and cleared into urine for urinalysis. It is demonstrated that the nanoreporter has high specificity for ctDNA mutation in peripheral blood, and urinalysis of cleared gamma PNA can provide valuable information for tumor progression and prognosis evaluation. This work demonstrates the potential of the nanoreporter for urinary monitoring of tumor and patient prognosis through in situ biosensing of ctDNA mutations. A DNA/gamma PNA hybrid nanoreporter is engineered for urinalysis of tumor-specific ctDNA mutations in peripheral blood with high selectivity. The specific recognition and recording of ctDNA mutations are accomplished by introducing gamma PNA into a DNA-based toehold-mediated strand displacement system. This work demonstrates the feasibility of employing nanoreporter for tumor urinalysis through in situ sensing and recording of ctDNA mutations in peripheral blood. image
资助项目	National University of Singapore [NUHSRO/2020/133/Startup/08, NUHSRO/2023/008/NUSMed/TCE/LOA, NUHSRO/2021/034/TRP/09/ Nanomedicine, NUHSRO/2021/044/Kickstart/09/LOA]; National Medical Research Council [MOH-OFIRG23jan-0005, CG21APR1005]; Singapore Ministry of Education [MOE-000387-00]; National Research Foundation [NRF-000352-00]; National Natural Science Foundation of China [22207013, 81972261, 82372128, 31971302]; Key project cultivation project of Wenzhou Medical University [KYYW202006]; Natural Science Foundation Distinguished Young Scholars grant of Guangdong Province [2023B1515020071]; Talent young scientist supporting program of China Association for Science and Technology; Educational Commission of Guangdong Province of China key Project [2020ZDZX2001]
出版者	WILEY
ISSN	2198-3844
EISSN	2198-3844
卷号	11 期号:33
DOI	10.1002/advs.202310225
页数	10
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:001260854100001
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20242716645389
EI主题词	Tumors
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.9 Biology ; 801.2 Biochemistry
URL	查看原文
PubMed ID	38958527
SCOPUSEID	2-s2.0-85197432333
通讯作者地址	[Sun, Weijian]Wenzhou Med Univ, Affiliated Hosp 2, Dept Gastrointestinal Surg, Wenzhou 325027, Peoples R China. ; [Sun, Weijian]Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China. ; [Chen, Xiaoyuan]Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Diagnost Radiol, Singapore 119074, Singapore. ; [Chen, Xiaoyuan]Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg Chem & Biomol Engn, Singapore 119074, Singapore. ; [Chen, Xiaoyuan]Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biomed Engn, Singapore 119074, Singapore. ; [Chen, Xiaoyuan]Natl Univ Singapore, Coll Design & Engn, Singapore 119074, Singapore. ; [Guo, Weisheng]Guangzhou Med Univ, Sch Biomed Engn, Dept Minimally Invas Intervent Radiol, State Key Lab Resp Dis, Guangzhou 510260, Peoples R China. ; [Guo, Weisheng]Guangzhou Med Univ, Affiliated Hosp 2, Guangzhou 510260, Peoples R China. ; [Chen, Xiaoyuan]Natl Univ Singapore, Clin Imaging Res Ctr, Ctr Translat Med, Yong Loo Lin Sch Med, Singapore 117599, Singapore. ; [Chen, Xiaoyuan]Natl Univ Singapore, Yong Loo Lin Sch Med, Nanomed Translat Res Program, Singapore 117597, Singapore. ; [Chen, Xiaoyuan]Natl Univ Singapore, Theranost Ctr Excellence TCE, Yong Loo Lin Sch Med, 11 Biopolis Way, Singapore 138667, Singapore. ; [Chen, Xiaoyuan]ASTAR, Inst Mol & Cell Biol, 61 Biopolis Dr, Singapore 138673, Singapore.
Scopus学科分类	Medicine (miscellaneous);Chemical Engineering (all);Materials Science (all);Biochemistry, Genetics and Molecular Biology (miscellaneous);Engineering (all);Physics and Astronomy (all)
SCOPUS_ID	SCOPUS_ID:85197432333
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/215203
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院附属第二医院_胃肠外科
通讯作者	Guo, Weisheng; Chen, Xiaoyuan; Sun, Weijian
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Gastrointestinal Surg, Wenzhou 325027, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China; 3.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Diagnost Radiol, Singapore 119074, Singapore; 4.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg Chem & Biomol Engn, Singapore 119074, Singapore; 5.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biomed Engn, Singapore 119074, Singapore; 6.Natl Univ Singapore, Coll Design & Engn, Singapore 119074, Singapore; 7.Guangzhou Med Univ, Sch Biomed Engn, Dept Minimally Invas Intervent Radiol, State Key Lab Resp Dis, Guangzhou 510260, Peoples R China; 8.Guangzhou Med Univ, Affiliated Hosp 2, Guangzhou 510260, Peoples R China; 9.Natl Univ Singapore, Clin Imaging Res Ctr, Ctr Translat Med, Yong Loo Lin Sch Med, Singapore 117599, Singapore; 10.Natl Univ Singapore, Yong Loo Lin Sch Med, Nanomed Translat Res Program, Singapore 117597, Singapore; 11.Natl Univ Singapore, Theranost Ctr Excellence TCE, Yong Loo Lin Sch Med, 11 Biopolis Way, Singapore 138667, Singapore; 12.ASTAR, Inst Mol & Cell Biol, 61 Biopolis Dr, Singapore 138673, Singapore
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 胃肠外科
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 胃肠外科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Xiang, Zhichu,Lu, Jianhua,Ming, Yang,et al. Engineering of a DNA/γPNA Hybrid Nanoreporter for ctDNA Mutation Detection via γPNA Urinalysis[J]. Advanced Science,2024,11(33).
APA	Xiang, Zhichu, Lu, Jianhua, Ming, Yang, Guo, Weisheng, Chen, Xiaoyuan, & Sun, Weijian. (2024). Engineering of a DNA/γPNA Hybrid Nanoreporter for ctDNA Mutation Detection via γPNA Urinalysis. Advanced Science, 11(33).
MLA	Xiang, Zhichu,et al."Engineering of a DNA/γPNA Hybrid Nanoreporter for ctDNA Mutation Detection via γPNA Urinalysis".Advanced Science 11.33(2024).