MicroRNA‑432 inhibits the aggressiveness of glioblastoma multiforme by directly targeting IGF‑1R

doi:10.3892/ijmm.2019.4429

科研成果详情

名称	MicroRNA‑432 inhibits the aggressiveness of glioblastoma multiforme by directly targeting IGF‑1R
作者	Chen, Xudong 1; Zhang, Xufei2; Sun, Shunjin 1; Zhu, Meixiao3
发表期刊	International Journal of Molecular Medicine
语种	英语
原始文献类型	Retracted
关键词	Glioblastoma multiforme IGF-1R MicroRNA-432
摘要	MicroRNA-432 (miR-432) has been studied in multiple tumors, but the expression status, biological functions and the mechanism of action of miR-432 in glioblastoma multiforme (GBM) are yet to be elucidated. In the present study, miR-432 expression in GBM was determined and its clinical significance was evaluated among patients with GBM. The effects on the malignancy of GBM in vitro and in vivo were examined in detail and the interactions between miR-432 and insulin-like growth factor 1 receptor (IGF‑1R) mRNA were then explored. miR-432 expression in GBM tissue samples and cell lines was measured by reverse transcription‑quantitative (RT‑q)PCR. GBM cell proliferation, apoptosis, migration and invasion in vitro and tumor growth in vivo were evaluated by a Cell Counting Kit‑8 assay, flow‑cytometric analysis, Transwell migration and invasion assays, and a tumor xenograft experiment, respectively. Bioinformatic analysis followed by a luciferase reporter assay, RT‑qPCR and western blotting was applied to demonstrate that IGF‑1R is a direct target gene of miR-432 in GBM cells. It was found that miR-432 is downregulated in GBM tumors and cell lines. miR-432 under expression obviously correlated with the Karnofsky Performance Status score and shorter overall survival among patients with GBM. Exogenous miR‑432 expression significantly reduced proliferation and induced apoptosis of GBM cells. In addition, miR‑432 overexpression impaired the migratory and invasive abilities of GBM cells in vitro and decreased their tumor growth in vivo. Furthermore, IGF‑1R was validated as a direct target gene of miR‑432 in GBM cells. IGF‑1R knockdown imitated the tumor‑suppressive actions of miR‑432 overexpression in GBM cells. Rescue experiments proved IGF‑1R downregulation to be essential for the effects of miR-432 on GBM cells. The results of the present study revealed a tumor‑suppressive role of the miR‑432‑IGF‑1R axis in GBM cells and this axis may have implications for GBM therapy.
出版者	Spandidos Publications
ISSN	1107-3756
EISSN	1791-244X
卷号	45
期号	2
页码	597-609
DOI	10.3892/ijmm.2019.4429
收录类别	SCOPUS
发布日期	2020
URL	查看原文
PubMed ID	31894251
通讯作者地址	[Zhu, Meixiao]Department of TCM Pharmacy,Sixth Affiliated Hospital,Wenzhou Medical University,15 Dazong Road,Lishui, Zhejiang,323000,China
Scopus学科分类	Genetics
SCOPUSEID	2-s2.0-85077767165
引用统计
文献类型	其他
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/214315
专题	温州医科大学实验动物中心
通讯作者	Zhu, Meixiao
作者单位	1.Department of Neurosurgery,Wenzhou Medical University,Lishui, Zhejiang,323000,China; 2.Laboratory Animal Center,Wenzhou Medical University,Wenzhou, Zhejiang,325035,China; 3.Department of TCM Pharmacy,Sixth Affiliated Hospital,Wenzhou Medical University,Lishui, Zhejiang,323000,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Chen, Xudong,Zhang, Xufei,Sun, Shunjin,et al. MicroRNA‑432 inhibits the aggressiveness of glioblastoma multiforme by directly targeting IGF‑1R. 2020.