Analysis of HPV16 E7 gene transcripts in cervical cancer and precancerous lesions

doi:10.3969/j.issn.1000-4718.2019.07.005

科研成果详情

题名	Analysis of HPV16 E7 gene transcripts in cervical cancer and precancerous lesions
其他题名	宫颈癌及癌前病变组织中含ＨＰＶ１６Ｅ７基因转录本分析
作者	Ji, Hong-Wei 1; Zhu, Hua 2; Lin, Cong 2; Zhang, Wen-Miao2; Chen, Jun3; Zhu, Xue-Jie2; Zou, Ruan-Min2
发表日期	2019
发表期刊	Chinese Journal of Pathophysiology 影响因子和分区
语种	中文
原始文献类型	Article
关键词	Cervical cancer HPV16 E7 gene Precancerous lesions Transcripts
摘要	AIM: To investigate the changes of human papillomavirus type 16 (HPV16) £"7-related transcripts at different lesion stages during the development of cervical cancer. METHODS; Amplication of papillomavirus oncogene transcripts (APOT) assay was used to detect E7 gene transcripts in 15 cases of low-grode squamous intraepithelial lesion (LSIL), 20 cases of high-grode squamous intraepithelial lesion (HSIL), 18 cases of cervical cancer, and 9 cases of normal HPV-negative cervix. The sequencing of transcripts and alignment analysis of the sequences were performed. Southern blot was used to verify the expression of £7-related transcripts in cervical cancer tissues. RESULTS: Compared with LSIL, more complex and diverse E7 transcripts in HSIL and cervical cancer tissues were observed, but no characteristic transcripts was found. Transcriptional diversity and accumulation were synchronized with the degree of canceration. Five kinds of HPV16 E7 transcriptional patterns were obtained. The patterns A, C and E were found for the first time. The patterns A and B were detected in all HPV16-positive cervical tissues, and were predominantly in the HSIL tissue. The pattern C was detected mostly in cervical cancer, and secondly in HSIL tissue, whereas the patterns D and E were found only in cervical cancer. The pattern D and E transcription contained E4 sequences, which were not detected in some cervical cancer specimens, and significant differences of E4 transcripts detected in different cervical cancer specimens were also observed. CONCLUSION; The transcriptional pattern of HPV16 E7 is different during various stages of cervical cancer. As the ingravescent canceration of the cells, some specific transcriptional patterns of the virus are gradually increased to form dominant transcription.
出版者	Editorial Board of Chinese Journal of Pathophysiology, Jinan University
ISSN	1000-4718
卷号	35 期号:7 页码:1183-1188
DOI	10.3969/j.issn.1000-4718.2019.07.005
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85174394321
通讯作者地址	[Zhu, Xue-Jie]Department of Obstetrics and Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Zou, Ruan-Min]Department of Obstetrics and Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Pathology and Forensic Medicine;Physiology (medical);Medicine (miscellaneous)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/214280
专题	附属第一医院基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zhu, Xue-Jie; Zou, Ruan-Min
作者单位	1.Department of Obstetrics and Gynecology,Lishui Central Hospital,Lishui,323000,China; 2.Department of Obstetrics and Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Microbiology and Immunology,Wenzhou Medical University,Wenzhou,325035,China
通讯作者单位	附属第一医院; 妇产科学; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Ji, Hong-Wei,Zhu, Hua,Lin, Cong,et al. Analysis of HPV16 E7 gene transcripts in cervical cancer and precancerous lesions[J]. Chinese Journal of Pathophysiology,2019,35(7):1183-1188.
APA	Ji, Hong-Wei., Zhu, Hua., Lin, Cong., Zhang, Wen-Miao., Chen, Jun., ... & Zou, Ruan-Min. (2019). Analysis of HPV16 E7 gene transcripts in cervical cancer and precancerous lesions. Chinese Journal of Pathophysiology, 35(7), 1183-1188.
MLA	Ji, Hong-Wei,et al."Analysis of HPV16 E7 gene transcripts in cervical cancer and precancerous lesions".Chinese Journal of Pathophysiology 35.7(2019):1183-1188.