Decidual macrophages modulate trophoblast cells by transferring exosomes in unexplained recurrent spontaneous abortion

doi:10.3969/j.issn.1000-4718.2019.09.027

科研成果详情

题名	Decidual macrophages modulate trophoblast cells by transferring exosomes in unexplained recurrent spontaneous abortion
其他题名	蜕膜巨噬细胞通过外泌体调控滋养细胞生物学行为参与不明原因复发性流产
作者	Ying, Xiang; Jin, Xiao-Xiao; Zhang, Zi-Juan; Wang, Kai; Ding, Chen-Jing; Zheng, Ling-Zhi
发表日期	2019
发表期刊	Chinese Journal of Pathophysiology 影响因子和分区
语种	中文
原始文献类型	Article
关键词	Decidual macrophages Exosomes Trophoblast cells Unexplained recurrent spontaneous abortion
摘要	Aim: To investigate whether decidual macrophages have ability to regulate trophoblast cells by secreting exosomes in unexplained recurrent spontaneous abortion (URSA). Methods: The decidual macrophages were isolated from deciduas of URSA and normal early pregnant induced abortion women. Transmission electron microscopy revealed the morphology and size of the macrophages-derived exosomes. Besides, the characterization of the exosomes was confirmed by the presence of known exosomal marker CD63 by Western blot. The HTR8/Snveo cells were treated with exosomes extracted from the decidual macrophages of URSA or normal early pregnanty induced abortion women. Confocal microscopy, MTS assay and Transwell assay were used to explore the effect of URSA macrophages-derived exosomes on viability and migration ability of trophoblasts cells. Results: Electron microscopy showed exosomes secreted by macrophages displayed a round-shaped appearance, ranging 40 ∼ 80 nm in diameter. The results of Western blot indicated that exosomes exacted from the macrophages expressed CD63, a member of tetraspanin family protein. Confocal microscopy revealed the exosomes were taken up by the HTR8/Snveo cells. MTS assay indicated the viability of HTR8/Snveo cells was declined when incubated with the URSA macrophages-derived exosomes (P < 0.05). In URSA macrophages-derived exosomes group, the migration ability of the HTR8/Snveo cells was decreased significantly as compared with normal early pregnant induced abortion macrophages-derived exosome (P < 0.05). Conclusion: Decidual macrophages of URSA inhibits the viability and migratory properties of trophoblast cells by exosomes, which participates in regulating maternal-fetal immune in URSA.
出版者	Editorial Board of Chinese Journal of Pathophysiology, Jinan University
ISSN	1000-4718
卷号	35 期号:9
DOI	10.3969/j.issn.1000-4718.2019.09.027
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85132339611
通讯作者地址	[Zheng, Ling-Zhi]Department of Obstetrics,Taizhou Hospital Affiliated to Wenzhou Medical University,Taizhou,317000,China
Scopus学科分类	Pathology and Forensic Medicine;Physiology (medical);Medicine (miscellaneous)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/214239
专题	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
通讯作者	Zheng, Ling-Zhi
作者单位	Department of Obstetrics,Taizhou Hospital Affiliated to Wenzhou Medical University,Taizhou,317000,China
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
推荐引用方式 GB/T 7714	Ying, Xiang,Jin, Xiao-Xiao,Zhang, Zi-Juan,et al. Decidual macrophages modulate trophoblast cells by transferring exosomes in unexplained recurrent spontaneous abortion[J]. Chinese Journal of Pathophysiology,2019,35(9).
APA	Ying, Xiang, Jin, Xiao-Xiao, Zhang, Zi-Juan, Wang, Kai, Ding, Chen-Jing, & Zheng, Ling-Zhi. (2019). Decidual macrophages modulate trophoblast cells by transferring exosomes in unexplained recurrent spontaneous abortion. Chinese Journal of Pathophysiology, 35(9).
MLA	Ying, Xiang,et al."Decidual macrophages modulate trophoblast cells by transferring exosomes in unexplained recurrent spontaneous abortion".Chinese Journal of Pathophysiology 35.9(2019).