Identification of core genes related to severity of respiratory syncytial virus bronchiolitis based on RNA sequencing data

doi:10.3760/cma.j.cn112309-20210825-00278

科研成果详情

题名	Identification of core genes related to severity of respiratory syncytial virus bronchiolitis based on RNA sequencing data
其他题名	基于 RNA 测序数据筛选与呼吸道合胞病毒毛细支气管炎疾病严重程度相关的核心基因
作者	Tingting, Weng 1; Leying, Wang 1; Haiyan, Li 1; Chunchan, Liang 1; Zhenwei, Liu 2; Lin, Dong 1
发表日期	2022-05-31
发表期刊	Chinese Journal of Microbiology and Immunology (China); 影响因子和分区
语种	中文
原始文献类型	Article
关键词	Bronchiolitis Disease severity Respiratory syncytial virus RNA-sequencing Weighted gene co-expression network analysis
摘要	Objective To identify the core genes related to the disease severity of respiratory syncytial virus (RSV) bronchiolitis in children using RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA), aiming to provide reference for predicting the condition of RSV infection. Methods Twenty-two patients admitted to the Second Affiliated Hospital of Wenzhou Medical University with RSV bronchiolitis from October 1, 2019 to February 29, 2020 were enrolled as the case group. They were divided into three groups based on the severity of the disease: mild group, moderate group and severe group. Twenty-two healthy children were selected as the control group. Total RNA was extracted from whole blood leukocytes and analyzed by RNA-seq to compare the differentially expressed genes (DEGs) between children with RSV bronchiolitis and healthy children. The gene co-expression modules related to disease severity and biological indicators for disease severity assessment were identified. Results The median age of the 22 patients (19 males and 3 females) was 3 months. The median age of the 22 healthy children (14 males and 8 females) was 4 months. There was no significant difference in age or gender between the two groups. There were 8 cases in the mild group, 7 cases in the moderate group and 7 cases in the severe group. Through significance analysis, 416 DEGs were found in the mild group, 586 in the moderate group and 846 in the severe group. According to WGCNA analysis, 10 co-expression modules were found, among which brown module (r=0. 62, P<0. 001) was significantly correlated with disease severity. The protein-protein interaction network of DEGs in brown module was constructed and the top 30 core genes were selected according to the connectivity of gene nodes, among which the genes with high correlation were RBX1 and PSMA7. The expression of RBX1 and PSMA7 genes was up-regulated in the severe group, but their expression in the mild and moderate groups was not significantly different from that in the control group. Conclusions RBX1 and PSMA7 genes might be biological predictors of disease severity in RSV bronchiolitis.
资助项目	Basic Scientific Research Project of Wenzhou[Y20190437];Second Affiliated Hospital and Yuying Children′s Hospital of Wenzhou Medical University[18331103];Basic Public Welfare Technology Research Project of Zhejiang Province[LGF20H010004];
ISSN	0254-5101
卷号	42 期号:5 页码:396-403
DOI	10.3760/cma.j.cn112309-20210825-00278
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85133349039
通讯作者地址	[Lin, Dong]Department of Pediatric Pulmonology,The Second Affiliated Hospital and Yuying Children′ s Hospital of Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Microbiology;Immunology;Virology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213993
专题	附属第二医院基因组医学研究院
通讯作者	Lin, Dong
作者单位	1.Department of Pediatric Pulmonology,The Second Affiliated Hospital and Yuying Children′ s Hospital of Wenzhou Medical University,Wenzhou,325027,China; 2.Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,325025,China
第一作者单位	附属第二医院
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Tingting, Weng,Leying, Wang,Haiyan, Li,et al. Identification of core genes related to severity of respiratory syncytial virus bronchiolitis based on RNA sequencing data[J]. Chinese Journal of Microbiology and Immunology (China);,2022,42(5):396-403.
APA	Tingting, Weng, Leying, Wang, Haiyan, Li, Chunchan, Liang, Zhenwei, Liu, & Lin, Dong. (2022). Identification of core genes related to severity of respiratory syncytial virus bronchiolitis based on RNA sequencing data. Chinese Journal of Microbiology and Immunology (China);, 42(5), 396-403.
MLA	Tingting, Weng,et al."Identification of core genes related to severity of respiratory syncytial virus bronchiolitis based on RNA sequencing data".Chinese Journal of Microbiology and Immunology (China); 42.5(2022):396-403.