Panax notoginseng saponins regulate autophagy and proliferation of rat PASMCs under hypoxia and hypercapnia environment by activating PI3K/AKT/mTOR signaling pathways

doi:10.3969/j.issn.1000-4718.2021.07.013

科研成果详情

题名	Panax notoginseng saponins regulate autophagy and proliferation of rat PASMCs under hypoxia and hypercapnia environment by activating PI3K/AKT/mTOR signaling pathways
其他题名	三七总皂苷通过激活 PI3K/AKT/mTOR 信号通路调控低氧高二氧化碳环境下大鼠 PASMCs 的自噬和增殖
作者	Chen, Ling-Long 1; Wu, Yuan-Ling 2,3; Zhang, Sai2; Huang, Dan-Na 2; Wang, Wan-Tie2
发表日期	2021-07-01
发表期刊	Chinese Journal of Pathophysiology; 影响因子和分区
语种	中文
原始文献类型	Article
关键词	Autophagy Cell proliferation Hypoxic hypercapnia-induced pulmonary hypertension Panax notoginseng saponins PI3K/AKT/mTOR signaling pathway Pulmonary artery smooth muscle cells
摘要	AIM:to investigate the effect of Panax notoginseng saponins(PNS)on autophagy and viability of rat pulmonary artery smooth muscle cells(PASMCs)under hypoxia and hypercapnia(HH)environment, and to explore the mechanism. METHODS:The rat PASMCs were randomly divided into 5 groups:control(CON)group, HH group, HH+PNS group, HH+LY294002(LY)group and HH+PNS+LY group. The cells in CON group were cultured in an nor-moxic cell incubator(21% O2, 5% CO2)for 24 h, and the cells in other 4 groups were terated with corresponding drugs and cultured in a modeling incubator(5% O2, 6% CO2)for 24 h. Cell viability was measured by CCK-8 assay. RT-qPCR was used to detect the mRNA levels of AKT, mTOR, LC3 and P62. The protein levels of p-AKT, p-mTOR, LC3B, P62 and proliferating cell nuclear antigen(PCNA)were detected by Western blot. Transmission electron microscopy was used to observed the changes in the internal ultrastructure of cells, such as autophagosomes. RESULTS:Compared with CON group, the viability of PASMCs in HH group was decreased, AKT, mTOR and P62 mRNA levels and p-AKT, p-mTOR and P62 protein levels were down-regulated, LC3 mRNA level and LC3B protein level were up-regulated, the number of autophagosomes was increased, and PCNA expression was up-regulated(P<0. 05). Compared with HH group, the viability of PASMCs in HH+PNS group was decreased, AKT, mTOR and P62 mRNA levels and p-AKT, p-mTOR and P62 protein levels were up-regulated, LC3 mRNA level and LC3B protein level were down-regulated(P<0. 05), the number of autophagosomes was decreased, and PCNA expression was down-regulated(P<0. 05). Compared with HH group, the viability of PASMCs in HH+LY group was decreased, AKT, mTOR and P62 mRNA levels and p-AKT, p-mTOR and P62 protein levels were down-regulated, LC3 mRNA level and LC3B protein level were up-regulated, the number of autophagosomes was increased, and PCNA expression was up-regulated(P<0. 05). Compared with HH+PNS group, the viability of PASMCs in HH+PNS+LY group was decreased, AKT, mTOR and P62 mRNA levels and p-AKT, p-mTOR and P62 protein levels were down-regulated, LC3 mRNA level and LC3B protein level were up-regulated, the number of autophagosomes was increased, and PCNA expression was up-regulated(P<0. 05). CONCLUSION:PNS may inhibit autophagy and proliferation of rat PASMCs under HH environment by activating PI3K/AKT/mTOR signal pathways.
ISSN	1000-4718
卷号	37 期号:7 页码:1246-1251
DOI	10.3969/j.issn.1000-4718.2021.07.013
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85168285331
通讯作者地址	[Wang, Wan-Tie]Department of Physiology and Pathophysiology,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Pathology and Forensic Medicine;Physiology (medical);Medicine (miscellaneous)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213931
专题	基础医学院（机能实验教学中心）_病理学与病理生理学系
通讯作者	Wang, Wan-Tie
作者单位	1.Department of Emergency Medicine,Wenzhou People's Hospital,Wenzhou,325000,China; 2.Department of Physiology and Pathophysiology,Wenzhou Medical University,Wenzhou,325035,China; 3.Clinical Lab,Shanxi Province Bethune Hospital,Taiyuan,030000,China
通讯作者单位	病理学与病理生理学系
推荐引用方式 GB/T 7714	Chen, Ling-Long,Wu, Yuan-Ling,Zhang, Sai,et al. Panax notoginseng saponins regulate autophagy and proliferation of rat PASMCs under hypoxia and hypercapnia environment by activating PI3K/AKT/mTOR signaling pathways[J]. Chinese Journal of Pathophysiology;,2021,37(7):1246-1251.
APA	Chen, Ling-Long, Wu, Yuan-Ling, Zhang, Sai, Huang, Dan-Na, & Wang, Wan-Tie. (2021). Panax notoginseng saponins regulate autophagy and proliferation of rat PASMCs under hypoxia and hypercapnia environment by activating PI3K/AKT/mTOR signaling pathways. Chinese Journal of Pathophysiology;, 37(7), 1246-1251.
MLA	Chen, Ling-Long,et al."Panax notoginseng saponins regulate autophagy and proliferation of rat PASMCs under hypoxia and hypercapnia environment by activating PI3K/AKT/mTOR signaling pathways".Chinese Journal of Pathophysiology; 37.7(2021):1246-1251.