Small ubiquitin-like modifier proteins specific protease 3 in microglia participating in the early progression of photothrombosic ischemic stroke mice model

doi:10.16098/j.issn.0529-1356.2021.04.008

科研成果详情

题名	Small ubiquitin-like modifier proteins specific protease 3 in microglia participating in the early progression of photothrombosic ischemic stroke mice model
其他题名	小胶质细胞中小泛素样修饰蛋白特异性蛋白酶３参与小鼠光化学栓塞法缺血性脑卒中早期的疾病进展
作者	Du, Juan-Juan; Ye, Zhen; Zhou, Tao; Ren, Yan-Hua; Uiao, Min
发表日期	2021-08-01
发表期刊	Acta Anatomica Sinica; 影响因子和分区
语种	中文
原始文献类型	Article
关键词	Microglia Mouse Photothrombosic ischemic stroke Small ubiquitin-like modifier proteins specific protease Western blotting
摘要	Objective To investigate the expression of small ubiquitin-like modifier proteins specific protease 3 (SENP3) in microglia of mice with ischemic stroke and its relationship with the progression of ischemic stroke. Methods The experimental animals were divided into control group, ischemic stroke day 1 group and ischemic stroke day 7 group (3 mice per group). The expression of inducible nitric oxide synthase (iNOS), argniase-1 (ARG-1), SENP3 and c-Jun N-terminal kinase (JNK) phosphorylation levels in the striatum were detected by immunoblotting. The expression of iNOS and ARG-1 in mouse striatum microglia was detected by immunofluorescence double labeling. Results Compared with the control group, the expression of SENP3 and the phosphorylation level of JNK in the ischemic stroke group increased significantly, and the expression of the marker iNOS of Ml type microglia increased significantly. The expression of the marker ARG-1 of M2 type microglia increased significantly in the day 7 group of ischemic stroke. The immunofluorescence double-labeled result of striatum ionized calcium binding adapter molecule 1 (Ibal) and iNOS, Ibal and ARG-1 were consistent with the result of immunoblotting. Conclusion In the early stage of ischemic stroke, the expression of SENP3 in microglia increases, which promote the cerebral inflammatory response by affecting the level of JNK phosphorylation and the polarization of microglia, and participate in the progression of ischemic stroke.
出版者	Editorial Board of Acta Anatomica Sinica
ISSN	0529-1356
卷号	52 期号:4 页码:548-553
DOI	10.16098/j.issn.0529-1356.2021.04.008
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85184763460
通讯作者地址	[Uiao, Min]Department of Histology and Embryology,Wenzhou Medical University,Wenzhou,Zhejiang,325035,China
Scopus学科分类	Anatomy;Structural Biology;Histology;Embryology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213693
专题	基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Uiao, Min
作者单位	Department of Histology and Embryology,Wenzhou Medical University,Zhejiang,325035,China
第一作者单位	组织胚胎学
通讯作者单位	组织胚胎学
第一作者的第一单位	组织胚胎学
推荐引用方式 GB/T 7714	Du, Juan-Juan,Ye, Zhen,Zhou, Tao,et al. Small ubiquitin-like modifier proteins specific protease 3 in microglia participating in the early progression of photothrombosic ischemic stroke mice model[J]. Acta Anatomica Sinica;,2021,52(4):548-553.
APA	Du, Juan-Juan, Ye, Zhen, Zhou, Tao, Ren, Yan-Hua, & Uiao, Min. (2021). Small ubiquitin-like modifier proteins specific protease 3 in microglia participating in the early progression of photothrombosic ischemic stroke mice model. Acta Anatomica Sinica;, 52(4), 548-553.
MLA	Du, Juan-Juan,et al."Small ubiquitin-like modifier proteins specific protease 3 in microglia participating in the early progression of photothrombosic ischemic stroke mice model".Acta Anatomica Sinica; 52.4(2021):548-553.