MicroRNA-875-5p inhibits the growth and metastasis of cervical cancer cells by promoting autophagy and apoptosis and inhibiting the epithelial-mesenchymal transition

doi:10.3389/fonc.2024.1361721

科研成果详情

题名	MicroRNA-875-5p inhibits the growth and metastasis of cervical cancer cells by promoting autophagy and apoptosis and inhibiting the epithelial-mesenchymal transition
作者	Liang, Yingxiu1; Li, Chunyang2; Hou, Xiaohong1; Lin, Yiguang 3,4,5; Cheng, Jing 1
发表日期	2024
发表期刊	Frontiers in Oncology; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	apoptosis autophagy cervical cancer microRNA tumor biomarker tumorigenesis
其他关键词	MIR-875-5P ; INVASION
摘要	Introduction: MicroRNA-875-5p (miR-875-5p) is a cancer-related microRNA. It has been demonstrated that miR−875−5p participates in the development of various types of cancer such as hepatocellular carcinoma, gastric carcinoma, prostate and bladder cancer. Previous research suggested that miR-875 is implicated in the development of cervical cancer cells. However, the exact role and function of miR−875−5p in cervical cancer remain unexplored. It is important to examine the role and function of miR-875-5p and the associated signaling pathway, as the findings may have diagnostic and therapeutic significance. Thus, in this study, we investigated the effect of miR-875-5p on the growth and metastasis of cervical cancer cells and the possible underlying mechanisms. Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-875-5p in cervical cancer cells and normal cervical epithelium. After overexpression or co-expression of miR-875-5p in cells, the changes in cell function were analyzed. Western blot was used to detect the expression changes of epithelial-mesenchymal transition (EMT) -related proteins and autophagy-related proteins. Results: Functional studies demonstrated that miR-875-5p overexpression significantly inhibited the proliferation, migration, invasion, and EMT, and promotes apoptosis and autophagy of cervical cancer cells., while miR-875-5p knockdown promoted the proliferation, migration, invasion, and EMT, and inhibited apoptosis and autophagy cervical cancer cells. Furthermore, Western blot results showed that overexpression of miR-875-5p downregulated the expressions of N-cadherin, Snail, Vimentin and microtubule-associated protein 1 light chain 3B I (LC3B I). Conversely, miR-875-5p upregulated the expression of E-cadherin. Conclusion: In conclusion, our findings suggest that miR-875-5p functions as a tumor inhibitor suppressing the growth and metastasis of cervical cancer. Overexpression of miR-875-5p inhibits malignant behavior and promotes autophagy and apoptosis in cervical cancer cells. These findings advance our understanding of the role and function of miR-875-5p in cervical cancer and could facilitate the development of early genetic markers or biomarkers and therapeutic targets for cervical cancer.
资助项目	Basic Public Welfare Research Plan of Zhejiang Province[LGF22H160053];Health Commission Project of Zhejiang Province[2022KY897];
出版者	FRONTIERS MEDIA SA
ISSN	2234-943X
卷号	14
DOI	10.3389/fonc.2024.1361721
页数	12
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:001230143700001
收录类别	SCOPUS ; SCIE ; PUBMED
URL	查看原文
PubMed ID	38800376
SCOPUSEID	2-s2.0-85193964047
通讯作者地址	[Cheng, Jing]Reproductive Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital and the Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China ; [Lin, Yiguang]School of Pharmaceutical Science,Guangdong Pharmaceutical University,Guangzhou,China
Scopus学科分类	Oncology;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213416
专题	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
通讯作者	Lin, Yiguang; Cheng, Jing
作者单位	1.Reproductive Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital and the Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Biochemistry,School of Basic Sciences,Wenzhou Medical University,Wenzhou,China; 3.School of Pharmaceutical Science,Guangdong Pharmaceutical University,Guangzhou,China; 4.Department of Traditional Chinese Medicine (TCM),Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou,China; 5.Research & Development Division,Guangzhou Anjie Biomedical Technology Co. Ltd,Guangzhou,China
推荐引用方式 GB/T 7714	Liang, Yingxiu,Li, Chunyang,Hou, Xiaohong,et al. MicroRNA-875-5p inhibits the growth and metastasis of cervical cancer cells by promoting autophagy and apoptosis and inhibiting the epithelial-mesenchymal transition[J]. Frontiers in Oncology;,2024,14.
APA	Liang, Yingxiu, Li, Chunyang, Hou, Xiaohong, Lin, Yiguang, & Cheng, Jing. (2024). MicroRNA-875-5p inhibits the growth and metastasis of cervical cancer cells by promoting autophagy and apoptosis and inhibiting the epithelial-mesenchymal transition. Frontiers in Oncology;, 14.
MLA	Liang, Yingxiu,et al."MicroRNA-875-5p inhibits the growth and metastasis of cervical cancer cells by promoting autophagy and apoptosis and inhibiting the epithelial-mesenchymal transition".Frontiers in Oncology; 14(2024).