Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry

doi:10.1002/rcm.3569

科研成果详情

题名	Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry
作者	Liu, Xin 1,2; Zhou, Yan 1; Chen, Haibin3; Peng, Shulin 1; Gu, Yucheng 2; Ding, Lisheng 1
发表日期	2008-06
发表期刊	RAPID COMMUNICATIONS IN MASS SPECTROMETRY 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	CIMICIFUGOSIDES ; DERIVATIVES ; OXOSTEROIDS ; RHIZOMES ; MS
摘要	A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine hydrochloride (NH2OH center dot HCl) as a derivatization reagent. We use the oxime formed during the derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups. Copyright (C) 2008 John Wiley & Sons, Ltd.
出版者	WILEY
ISSN	0951-4198
EISSN	1097-0231
卷号	22 期号:12 页码:1981-1992
DOI	10.1002/rcm.3569
页数	12
WOS类目	Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
WOS记录号	WOS:000256985100025
收录类别	SCIE
PubMed ID	18494003
通讯作者地址	[Zhou, Yan]Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213321
专题	公共卫生学院_健康与环境生态研究所
通讯作者	Zhou, Yan
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; 2.Syngenta Jealotts Hill Int Res Ctr, Bracknell RG42 6EY, Berks, England; 3.Wenzhou Med Coll, Inst Hlth & Environm, Wenzhou 325035, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xin,Zhou, Yan,Chen, Haibin,et al. Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(12):1981-1992.
APA	Liu, Xin, Zhou, Yan, Chen, Haibin, Peng, Shulin, Gu, Yucheng, & Ding, Lisheng. (2008). Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 22(12), 1981-1992.
MLA	Liu, Xin,et al."Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22.12(2008):1981-1992.