| 题名 | Colloidal quantum dots-modified electrochemical sensor for high-sensitive extracellular vesicle detection |
| 作者 | |
| 发表日期 | 2024-05-01 |
| 发表期刊 | Chemical Engineering Journal 影响因子和分区 |
| 语种 | 英语 |
| 原始文献类型 | Journal article (JA) |
| 关键词 | Chemical detection Diagnosis Electric discharges IV-VI semiconductors Lead compounds Medical applications Nanocrystals Proteins Semiconductor quantum dots Voltammetry Biological fluids Biomedical applications Colloidal quantum dots Detecting trace Exosomes Extracellular Extracellular vesicle Point of care Point-of-care test Precise diagnostics |
| 其他关键词 | EXOSOMES |
| 摘要 | Small extracellular vesicles (sEVs), primarily referring to exosomes, have emerged as potent tools for investigating pathological mechanisms and facilitating precise diagnostics owing to their ability to transfer informative molecules between cells and their accessibility from diverse biological fluids. Despite the multitude of biomedical applications, achieving enhanced specificity and sensitivity in detecting trace sEV samples continues to pose a significant challenge. Considering the potential of electrochemical sensing in efficient molecular detection, this study proposes a viable strategy for specifically recognizing sEVs by targeting their membrane proteins. Additionally, it demonstrates sensitive and quantitative detection of sEVs based on the unique performance of PbS colloidal quantum dots (CQDs) nanomaterials. The good immobilization of target proteins on the chemically modified electrode is achieved through the synergistic effect of the charge–discharge mechanism and strong suspension bonds of PbS CQDs. The results obtained from the analysis of differential pulse voltammetry demonstrate rapid and highly sensitive electrochemical responses. The CD63 antibody-modified sensor can achieve highly sensitive detection of sEVs, providing a wide linear detection range (102 ∼ 108 particles mL−1) and a low detection limit (19 particles mL−1). The established method is ultimately employed to differentiate the sEVs originating from distinct tumor cells, thereby demonstrating its potential in membrane-biomarker-based diagnostics. Consequently, the presented electrochemical sensor demonstrates remarkable efficiency in quantifying EVs with exceptional specificity and sensitivity, thereby offering extensive prospects for applications in EV analysis and point-of-care tests. © 2024 Elsevier B.V. |
| 资助项目 | Zhejiang Provincial Natural Science Foundation of China[Y23F040019];Hubei Provincial Natural Science Foundation of China[2022CFA035]; |
| 出版者 | Elsevier B.V. |
| ISSN | 1385-8947 |
| EISSN | 1873-3212 |
| 卷号 | 487 |
| DOI | 10.1016/j.cej.2024.150616 |
| 页数 | 9 |
| WOS类目 | Engineering, Environmental ; Engineering, Chemical |
| WOS研究方向 | Engineering |
| WOS记录号 | WOS:001216083300001 |
| 收录类别 | EI ; SCIE ; SCOPUS |
| 在线发表日期 | 2024-03 |
| EI入藏号 | 20241315828978 |
| EI主题词 | Electrochemical sensors |
| EI分类号 | 461.6 Medicine and Pharmacology ; 701.1 Electricity: Basic Concepts and Phenomena ; 712.1 Semiconducting Materials ; 714.2 Semiconductor Devices and Integrated Circuits ; 732.2 Control Instrumentation ; 761 Nanotechnology ; 801 Chemistry ; 801.4.1 Electrochemistry ; 804.1 Organic Compounds ; 933.1 Crystalline Solids |
| URL | 查看原文 |
| Scopus记录号 | 2-s2.0-85188800622 |
| 通讯作者地址 | [Hu, Liang]School of Integrated Circuits,Wuhan National Laboratory for Optoelectronics,Optics Valley Laboratory,Huazhong University of Science and Technology,Hubei,Wuhan,430074,China |
| scopus学科分类 | Chemistry (all);Environmental Chemistry;Chemical Engineering (all);Industrial and Manufacturing Engineering |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/211373 |
| 专题 | 眼视光学院（生物医学工程学院）、附属眼视光医院 |
| 通讯作者 | Hu, Liang |
| 作者单位 | 1.School of Integrated Circuits,Wuhan National Laboratory for Optoelectronics,Optics Valley Laboratory,Huazhong University of Science and Technology,Hubei,Wuhan,430074,China; 2.National Engineering Research Center of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 3.The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory,Systems Biology Theme,Department of Biomedical Engineering,College of Life Science and Technology,Huazhong University of Science and Technology,Hubei,Wuhan,430074,China; 4.Department of Laboratory Medicine,Maternal and Child Health Hospital of Hubei Province,Tongji Medical College,Huazhong University of Science and Technology,Hubei,Wuhan,430070,China; 5.Wenzhou Advanced Manufacturing Institute,Huazhong University of Science and Technology,Zhejiang,Wenzhou,325035,China |
| 推荐引用方式 GB/T 7714 | Huang, Jing,Chen, Tucan,Zhao, Yunong,et al. Colloidal quantum dots-modified electrochemical sensor for high-sensitive extracellular vesicle detection[J]. Chemical Engineering Journal,2024,487. |
| APA | Huang, Jing., Chen, Tucan., Zhao, Yunong., Li, Dandan., Huang, Qing., ... & Liu, Huan. (2024). Colloidal quantum dots-modified electrochemical sensor for high-sensitive extracellular vesicle detection. Chemical Engineering Journal, 487. |
| MLA | Huang, Jing,et al."Colloidal quantum dots-modified electrochemical sensor for high-sensitive extracellular vesicle detection".Chemical Engineering Journal 487(2024). |
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