Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

doi:10.3389/fmicb.2024.1387855

科研成果详情

题名	Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China
作者	Huang, Linyao1; Zhu, Liangrong 2; Yan, Jianxin 1; Lin, Yajing 1; Ding, Ding 1; He, Long 1; Li, Yexuzi3; Ying, Yi 4; Shen, Lijiong 1; Jiang, Yuhan 5; Cai, Haijun6; Jiang, Tian1
发表日期	2024-04-04
发表期刊	Frontiers in microbiology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	MRSA country-level hospitals molecular epidemiology nosocomial outbreak transmission whole-genome sequencing
其他关键词	INFECTIONS ; CLONES ; PVL
摘要	Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen contributing to healthcare-associated infections, which can result in multiple sites infections. The epidemiological characteristics of MRSA exhibit variability among distinct regions and healthcare facilities. The aim of this study was to investigate the molecular epidemiology and nosocomial outbreak characteristics of MRSA in a county-level hospital in China. A total of 130 non-repetitive MRSA strains were collected from December 2020 to November 2021. Whole-genome sequencing (WGS) was performed to identify antimicrobial resistance and virulence factors. Phylogenetic analysis was conducted to ascertain genetic diversity and phylogenetic relationships. Independent transmission scenarios were determined by the phylogeny derived from single nucleotide polymorphisms (SNPs) within the core genome. All the MRSA isolates were collected from the intensive care unit (30.00%, 39/130), the department of otorhinolaryngology (10.00%, 13/130) and the department of burn unit (9.23%, 12/130). The clinical samples mainly included phlegm (53.85%, 70/130), purulent fluid (24.62%, 32/130), and secretions (8.46%, 11/130). The resistance rates to erythromycin, clindamycin and ciprofloxacin were 75.38, 40.00, and 39.23%, respectively. All the isolates belonged to 11 clonal complexes (CCs), with the major prevalent types were CC5, CC59, and CC398, accounting for 30.00% (39/130), 29.23% (38/130), and 16.92% (22/130), respectively. Twenty sequence types (STs) were identified, and ST59 (25.38%, 33/130) was the dominant lineage, followed by ST5 (23.84%, 31/130) and ST398 (16.92%, 22/130). Three different SCCmec types were investigated, most of isolates were type IV (33.85%, 44/130), followed by type II (27.69%, 36/130) and type III (0.77%, 1/130). The common clonal structures included CC5-ST5-t2460-SCCmec IIa, CC59-ST59-t437-SCCmec IV and CC398-ST398-t034-SCCmec (-), with rates of 16.92% (22/130), 14.62% (19/130), and 13.84% (18/130), respectively. Only 12 panton-valentine leucocidin (PVL) positive strains were identified. Two independent clonal outbreaks were detected, one consisting of 22 PVL-negative strains belongs to CC5-ST5-t2460-SCCmec IIa and the other consisting of 8 PVL-negative strains belongs to CC5-ST5-t311-SCCmec IIa. Overall, our study indicated that the CC5 lineage emerged as the predominant epidemic clone of MRSA, responsible for nosocomial outbreaks and transmission within a county-level hospital in China, highlighting the necessity to strengthen infection control measures for MRSA in such healthcare facilities
资助项目	Scientific Research Fund of Taizhou Science and Technology Bureau[22ywb125];Zhejiang Provincial Medical and Health Science and Technology Project[2023XY088,2022RC299];Zhejiang Provincial Program for the Cultivation of New Health Talents[2020-3-187];Wenling Science and Technology Bureau[2021S00229];Taizhou City Program for the High-level Talent Special Support Plan[2022-3-18];
出版者	FRONTIERS MEDIA SA
ISSN	1664-302X
EISSN	1664-302X
卷号	15
DOI	10.3389/fmicb.2024.1387855
页数	11
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001205074400001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	38638904
SCOPUSEID	2-s2.0-85190606230
通讯作者地址	[Jiang, Tian]Department of Clinical Laboratory,The First People's Hospital of Wenling,Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China ; [Cai, Haijun]Burn Unit,The First People's Hospital of Wenling,Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China
Scopus学科分类	Microbiology;Microbiology (medical)
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/211165
专题	其他_附属温岭医院（温岭市第一人民医院）
通讯作者	Cai, Haijun; Jiang, Tian
作者单位	1.Department of Clinical Laboratory,The First People's Hospital of Wenling,Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China; 2.Department of Pharmacy,Wenling Hospital of Traditional Chinese Medicine,Affiliated Wenling Traditional Chinese Medicine Hospital,Zhejiang Chinese Medical University,Wenling,China; 3.Department of Critical Care Medicine,The First People's Hospital of Wenling,Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China; 4.Department of Traditional Chinese Medicine,The Affiliated Xianju’s Hospital,Hangzhou Medical College,Xianju,China; 5.School of Public Health,Hangzhou Medical College,Hangzhou,China; 6.Burn Unit,The First People's Hospital of Wenling,Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China
通讯作者单位	其他_附属温岭医院（温岭市第一人民医院）
推荐引用方式 GB/T 7714	Huang, Linyao,Zhu, Liangrong,Yan, Jianxin,et al. Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China[J]. Frontiers in microbiology,2024,15.
APA	Huang, Linyao., Zhu, Liangrong., Yan, Jianxin., Lin, Yajing., Ding, Ding., ... & Jiang, Tian. (2024). Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China. Frontiers in microbiology, 15.
MLA	Huang, Linyao,et al."Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China".Frontiers in microbiology 15(2024).