Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry

doi:10.2174/0115734129280521240110094134

科研成果详情

题名	Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry
作者	Zhang, Meiling1; Dong, Xicheng 1; Wang, Wanhang 2; Wang, Ziyue 2; Chen, Dizhong 2; Wen, Congcong 2; Wang, Xianqin1
发表日期	2024
发表期刊	Current Pharmaceutical Analysis; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	2-norfluoro-ketamine fluoroketamine Ketamine norketamine rat urine UPLC-MS/MS
摘要	Objective: This study aimed to establish a simple, reliable, and sensitive method for detecting ketamine, fluoroketamine, and their metabolites in urine using UPLC-MS/MS. Methods: The chromatographic separation was performed on UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) at a column temperature of 40°C. The mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile, with a flow rate set at 0.4 mL/min, following a specific elution procedure. A urine sample was treated with acetonitrile, and midazolam was used as an internal standard. Multiple reaction monitoring was used for quantitative analysis. Results: Ketamine, fluoroketamine, norketamine, and 2-norfluoro-ketamine exhibited linearity in urine (r>0.99) within the concentration range of 5-2000 ng/mL. Intra-day and inter-day precisions were 9% or less and 12% or less, respectively. The accuracy ranged from 92 to 107%. Mean recoveries were above 76%. The measured matrix effect was between 85 and 104%. Conclusion: This simple, reliable, and sensitive PLC-MS/MS method was successfully developed to determine ketamine, fluoroketamine, and their metabolite in rat urine.
资助项目	Wenzhou Science and Technology Bureau [Y2020069]
出版者	BENTHAM SCIENCE PUBL LTD
ISSN	1573-4129
EISSN	1875-676X
卷号	20 期号:1 页码:22-28
DOI	10.2174/0115734129280521240110094134
页数	7
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:001191209000002
收录类别	SCOPUS ; SCIE
URL	查看原文
SCOPUSEID	2-s2.0-85186939896
通讯作者地址	[Wang, Xianqin]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Biophysics;Biochemistry;Molecular Medicine;Pharmaceutical Science
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210653
专题	药学院（分析测试中心）实验动物中心
通讯作者	Wang, Xianqin
作者单位	1.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China; 2.Laboratory Animal Centre,Wenzhou Medical University,Wenzhou,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Zhang, Meiling,Dong, Xicheng,Wang, Wanhang,et al. Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry[J]. Current Pharmaceutical Analysis;,2024,20(1):22-28.
APA	Zhang, Meiling., Dong, Xicheng., Wang, Wanhang., Wang, Ziyue., Chen, Dizhong., ... & Wang, Xianqin. (2024). Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry. Current Pharmaceutical Analysis;, 20(1), 22-28.
MLA	Zhang, Meiling,et al."Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry".Current Pharmaceutical Analysis; 20.1(2024):22-28.