Hsacirc00 16600 通过调控 miR-149/PTK7 轴促进甲状腺癌细胞迁移的机制

doi:10.3969/j.issn.2095-9400.2024.02.005

科研成果详情

题名	Hsacirc00 16600 通过调控 miR-149/PTK7 轴促进甲状腺癌细胞迁移的机制
其他题名	The mechanism of hsa_circ_0016600 promoting thyroid cancer cell migration via modulating the miR-149/PTK7 axis
作者	张毅; 李飞蕾; 董剑达; 朱少俊; 孙艺晗
发表日期	2024-02-28
发表期刊	温州医科大学学报影响因子和分区
语种	中文
原始文献类型	Periodical
关键词	甲状腺癌环状RNAs 微小RNAs PTK7 细胞迁移
其他关键词	thyroid cancer ; hsa_circ_0016600 ; miR-149 ; PTK7 ; cell migration
摘要	目的:探究环状RNA hsa_circ_0016600对甲状腺癌细胞迁移的影响及其作用机制.方法:收集温州医科大学附属第二医院颈部外科收治的15例甲状腺癌患者的癌组织及正常组织标本,采用RT-qPCR法检测hsa_circ_0016600、miR-149和PTK7在甲状腺癌组织及甲状腺细胞系中的表达情况.体外培养甲状腺癌细胞系,分别敲低hsa_circ_0016600和过表达miR-149,采用CCK-8、Transwell和划痕实验检测甲状腺细胞增殖、迁移和侵袭能力.建立小鼠异种移植皮下瘤模型,观察敲低hsa_circ_0016600的甲状腺癌细胞在体内的成瘤作用,并利用荧光原位杂交实验检测hsa_circ_0016600在裸鼠移植瘤模型中的表达水平.采用RNA荧光原位杂交实验检测hsa_circ_0016600和miR-149在甲状腺癌细胞中的定位,双荧光素酶报告基因实验检测hsa_circ_0016600与miR-149及miR-149与PTK7的靶向调控关系.结果:在人甲状腺癌组织和细胞系中,hsa_circ_0016600(P＜0.05)和 PTK7 表达上调(P＜0.01),miR-149 表达下调(P＜0.05).敲低 hsa_circ_0016600表达后,甲状腺癌细胞的增殖(P＜0.01)、侵袭(P＜0.01)和迁移能力(P＜0.01)受到抑制.hsa_circ_0016600可通过靶向miR-149促进甲状腺癌细胞的增殖(P＜0.01)、迁移(P＜0.01)和血管形成,并上调PTK7的表达(P＜0.01).此外,PTK7是miR-149的一个靶基因.结论:Hsa_circ_0016600在甲状腺癌组织和细胞中高表达,并通过调控miR-149/PTK7轴促进甲状腺癌细胞的迁移.
其他摘要	Objective:To investigate the impact of circular RNA hsa_circ_0016600 on the migration of thyroid cancer cells and its mechanisms.Methods:Cancer tissues and normal tissue specimens from 15 thyroid cancer patients treated at the Department of Pate Surgery,the Second Affiliated Hospital of Wenzhou Medical University.Utilize RT-qPCR to assess the expression levels of hsa_circ_0016600,miR-149,and PTK7 in both thyroid cancer tissues and thyroid cell lines.Thyroid cancer cell lines were cultured in vitro,and knockdown experiments were conducted for hsa_circ_0016600 and overexpression experiments for miR-149.Cell proliferation,migration,and invasion capabilities were assessed using CCK-8 assays,transwell assays,and scratch assays.A xenograft subcutaneous tumor model in mice was established to observe the tumorigenic effect of thyroid cancer cells with hsacirc00 16600 knockdown ini vivo.Fluorescence in situ hybridization experiments were conducted to assess the expression level of hsa_circ_0016600 in the xenograft tumor model in nude mice.RNA fluorescence in situ hybridization experiments were performed to determine the cellular localization of hsa_circ_0016600 and miR-149 in thyroid cancer cells.Dual-luciferase reporter gene assays were used to investigate the targeted regulatory relationships between hsa_circ_0016600 and miR-149,as well as miR-149 and PTK7.Results:In human thyroid cancer tissues and cell lines,the expression of hsa_circ_0016600(P＜0.05)and PTK7(P＜0.01)was upregulated,while the expression of miR-149(P＜0.05)downregulated.Knockdown of hsa_circ_0016600 expression inhibited the proliferation(P＜0.01),invasion(P＜0.01),and migration(P＜0.01)capabilities of thyroid cancer cells.Hsa_circ_0016600 could promote the proliferation(P＜0.01),migration(P＜0.01),and angiogenesis of thyroid cancer cells by targeting miR-149 and upregulating the expression of PTK7(P＜0.01).Additionally,PTK7 was a target gene of miR-149.Conclusion:Hsa_circ_0016600 is highly expressed in thyroid cancer cells and promotes thyroid cancer cell migration by targeting regulation of the miR-149/PTK7 axis.
资助项目	Y20211115:温州市基础性科研项目
ISSN	2095-9400
卷号	54 期号:2 页码:120-127,134
DOI	10.3969/j.issn.2095-9400.2024.02.005
页数	9
收录类别	万方
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引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210616
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院附属第二医院_外科
作者单位	温州医科大学附属第二医院颈部外科,浙江温州 325027
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 外科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	张毅,李飞蕾,董剑达,等. Hsacirc00 16600 通过调控 miR-149/PTK7 轴促进甲状腺癌细胞迁移的机制[J]. 温州医科大学学报,2024,54(2):120-127,134.
APA	张毅, 李飞蕾, 董剑达, 朱少俊, & 孙艺晗. (2024). Hsacirc00 16600 通过调控 miR-149/PTK7 轴促进甲状腺癌细胞迁移的机制. 温州医科大学学报, 54(2), 120-127,134.
MLA	张毅,et al."Hsacirc00 16600 通过调控 miR-149/PTK7 轴促进甲状腺癌细胞迁移的机制".温州医科大学学报 54.2(2024):120-127,134.