Electroacupuncture promotes macrophage/microglial M2 polarization and suppresses inflammatory pain through AMPK

doi:10.1097/WNR.0000000000002005

科研成果详情

题名	Electroacupuncture promotes macrophage/microglial M2 polarization and suppresses inflammatory pain through AMPK
作者	Nan, Fu-Bei1; Gu, Yi-Xiao 2; Wang, Jun-Lu1; Chen, Shuang-Dong1
发表日期	2024-03-20
发表期刊	Neuroreport 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	AMPK electroacupuncture inflammatory pain microglia polarization
其他关键词	MEDIATOR-INDUCED SENSITIZATION
摘要	Inflammatory pain, the most prevalent disease globally, remains challenging to manage. Electroacupuncture emerges as an effective therapy, yet its underlying mechanisms are not fully understood. This study investigates whether adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)-regulated silent information regulator 1 (SIRT1) contributes to electroacupuncture's antinociceptive effects by modulating macrophage/microglial polarization in the spinal dorsal horn of a mouse model of inflammatory pain. In this study, mice, introduced to inflammatory pain through subcutaneous injections of complete freund's adjuvant (CFA) in the plantar area, underwent electroacupuncture therapy every alternate day for 30-min sessions. The assessment of mechanical allodynia and thermal hyperalgesia in these subjects was carried out using paw withdrawal frequency and paw withdrawal latency measurements, respectively. Western blot analysis measured levels of AMPK, phosphorylation-adenosine 5'-monophosphate (AMP)-activated protein kinase, SIRT1, inducible nitric oxide synthase, cluster of differentiation 86, arginase 1, and interleukin 10. In contrast to the group treated solely with CFA, the cohort receiving both CFA and electroacupuncture demonstrated notable decreases in both thermal hyperalgesia and mechanical allodynia. This was accompanied by a marked enhancement in AMPK phosphorylation levels. AMPK knockdown reversed electroacupuncture's analgesic effects and reduced M2 macrophage/microglial polarization enhancement. Additionally, AMPK knockdown significantly weakened electroacupuncture-induced SIRT1 upregulation, and EX-527 injection attenuated electroacupuncture's facilitation of M2 macrophage/microglial polarization without affecting AMPK phosphorylation levels. Furthermore, combining electroacupuncture with SRT1720 enhanced the analgesic effect of SRT1720. Our findings suggest that AMPK regulation of SIRT1 plays a critical role in electroacupuncture's antinociceptive effect through the promotion of M2 macrophage/microglial polarization.
资助项目	Zhejiang Provincial Natural Science Foundation of China[LQ21H290006];
出版者	LIPPINCOTT WILLIAMS & WILKINS
ISSN	0959-4965
EISSN	1473-558X
卷号	35 期号:6 页码:343-351
DOI	10.1097/WNR.0000000000002005
页数	9
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:001233735500006
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	38526969
SCOPUSEID	2-s2.0-85189354039
通讯作者地址	[Chen, Shuang-Dong]Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang Street, Ouhai District, Zhejiang,Wenzhou,325035,China
Scopus学科分类	Neuroscience (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210381
专题	附属第一医院其他_附属台州医院（浙江省台州医院）
通讯作者	Chen, Shuang-Dong
作者单位	1.Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Anesthesiology,Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University,Zhejiang,Taizhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Nan, Fu-Bei,Gu, Yi-Xiao,Wang, Jun-Lu,et al. Electroacupuncture promotes macrophage/microglial M2 polarization and suppresses inflammatory pain through AMPK[J]. Neuroreport,2024,35(6):343-351.
APA	Nan, Fu-Bei, Gu, Yi-Xiao, Wang, Jun-Lu, & Chen, Shuang-Dong. (2024). Electroacupuncture promotes macrophage/microglial M2 polarization and suppresses inflammatory pain through AMPK. Neuroreport, 35(6), 343-351.
MLA	Nan, Fu-Bei,et al."Electroacupuncture promotes macrophage/microglial M2 polarization and suppresses inflammatory pain through AMPK".Neuroreport 35.6(2024):343-351.