Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption

doi:10.1159/000537772

科研成果详情

题名	Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption
作者	Lin, Lina1; Gao, Wei1; Feng, Linya 1; Wang, Chundong2; Yang, Ruiqi3; Wang, Weijian1; Wu, Qiaolin1
发表日期	2024-04
发表期刊	Journal of vascular research 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Autophagy Glycocalyx Low shear stress MMP-2 and MMP-9
其他关键词	MATRIX METALLOPROTEINASES ; CARDIOPULMONARY BYPASS ; ARTERIAL ENLARGEMENT ; HEPARAN-SULFATE ; CELL AUTOPHAGY ; EXPRESSION ; ACTIVATION
摘要	Previous studies have confirmed that low shear stress (LSS) induces glycocalyx disruption, leading to endothelial dysfunction. However, the role of autophagy in LSS-induced glycocalyx disruption and relevant mechanism are not clear. In this study, we hypothesized that LSS may promote autophagy, disrupting the endothelium glycocalyx., Human umbilical vein endothelial cells were subjected to physiological shear stress and LSS treatments, followed by the application of autophagy inducers and inhibitors. Additionally, cells were treated with specific matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) inhibitor. The expression of autophagic markers, glycocalyx, MMP-2, and MMP-9 was measured., LSS impacted the expression of endothelium autophagy markers, increasing the expression of LC3II.LC3I-1 and Beclin-1, and decreasing the levels of p62, accompanied by glycocalyx disturbance. Moreover, LSS upregulated the expression of MMP-2 and MMP-9 and downregulated the levels of syndecan-1 and heparan sulfate (HS). Additionally, expression of MMP-2 and MMP-9 was increased by an autophagy promoter but was decreased by autophagy inhibitor treatment under LSS. Autophagy and MMP-2 and MMP-9 further caused glycocalyx disruption., LSS promotes autophagy, leading to glycocalyx disruption. Autophagy increases the expression of MMP-2 and MMP-9, which are correlated with the glycocalyx destruction induced by LSS.
资助项目	Wenzhou Science and Technology Bureau [Y2020160]; Wenzhou Science and Technology Bureau [Y2020162]
出版者	KARGER
ISSN	1018-1172
EISSN	1423-0135
卷号	61 期号:2 页码:77-88
DOI	10.1159/000537772
页数	12
WOS类目	Physiology ; Peripheral Vascular Disease
WOS研究方向	Physiology ; Cardiovascular System & Cardiology
WOS记录号	WOS:001189611900001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2024-03
URL	查看原文
PubMed ID	38503274
SCOPUSEID	2-s2.0-85190175318
通讯作者地址	[Wang, Weijian]Department of Anaesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China ; [Wu, Qiaolin]Department of Anaesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Physiology;Cardiology and Cardiovascular Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210315
专题	附属第一医院其他_附属东阳医院（东阳市人民医院）
通讯作者	Wang, Weijian; Wu, Qiaolin
作者单位	1.Department of Anaesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Anaesthesiology,Dongyang Hospital Affiliated to Wenzhou Medical University,Jinhua,China; 3.Department of the Operating Room,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Lina,Gao, Wei,Feng, Linya,et al. Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption[J]. Journal of vascular research,2024,61(2):77-88.
APA	Lin, Lina., Gao, Wei., Feng, Linya., Wang, Chundong., Yang, Ruiqi., ... & Wu, Qiaolin. (2024). Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption. Journal of vascular research, 61(2), 77-88.
MLA	Lin, Lina,et al."Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption".Journal of vascular research 61.2(2024):77-88.