A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice

doi:10.1016/j.aca.2024.342434

科研成果详情

题名	A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice
作者	Huang, Zu-Sheng1; Zhang, Wenxuan1; Liang, Manshan1; Wang, Shihua1; Zhang, Zhongda1; Jiang, Yongsheng2; Ye, Xiaoxia1; Xie, Longteng 2; Quan, Yun-Yun1
发表日期	2024-04-22
发表期刊	Analytica chimica acta 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Fluorescent probe Hydrogen sulfide Multifunctional Sequential detection pH
摘要	Cancer as a leading cause of premature death worldwide has become a major threat to human health due to the high incidence and mortality. Monitoring tumor markers are reliable and significantly important for early detection of cancers. In complex biological systems, it is of great urgency but still remains challenging to conceive a fluorescent probe with multiple tumor markers detection property. Hydrogen sulfide (H2S) and pH are two target biomarkers for diagnosis of early cancer. The preparation of a novel probe with H2S and pH dual detection functions is highly anticipated.}, {AbstractText=Herein, a novel sequential detection probe HTPQ-HS for H2S and pH has been developed. In this system, HPQ (2-(2 -hydroxyphenyl)-4(3H)-quinazolinone) structure combined with triphenylamine is applied as the fluorophore, and 2, 4-dinitrophenylsulfonyl group is used as the recognition group. In the presence of H2S, HTPQ-HS is transformed into product HTPQ-OH which shows fluorescence enhancement (29-fold) at 525 nm in less than 4 min and further displays repeatable acid-base responsive ability. HTPQ-HS is able to sequentially response to H2S and pH in living cells and does not react directly with pH. Owing to the low cytotoxicity, HTPQ-HS is able to detect exogenous and endogenous H2S in colon cancer cells and mice, monitor H2S in inflammation model and in foodstuffs. As the environment changes from acidic to alkaline, the fluorescence intensity ratio (I470/I530) of product HTPQ-OH changes remarkably, illustrating the ratiometric fluorescent responsiveness to pH.}, {AbstractText=A multifunctional fluorescent probe HTPQ-HS for sequential detection of H2S and pH is synthesized. Probe HTPQ-OH realizes the monitoring of dynamic changes in intracellular pH and displays prospective application in security printing. We expect that our work could offer an important guidance on the development of multifunctional fluorescent probes for visualizing H2S and pH in biology and environment
资助项目	Public Science and Technology Project[S20210007,S20220020];National Natural Science Foundation of China[21702147];
出版者	Elsevier B.V.
ISSN	0003-2670
EISSN	1873-4324
卷号	1299
DOI	10.1016/j.aca.2024.342434
页数	11
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:001209391300001
收录类别	PUBMED ; EI ; SCOPUS ; SCIE
EI入藏号	20241015666100
EI主题词	Hydrogen sulfide
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.7 Health Care ; 741.1 Light/Optics ; 741.3 Optical Devices and Systems ; 801 Chemistry ; 801.1 Chemistry, General ; 804.2 Inorganic Compounds ; 941.3 Optical Instruments ; 941.4 Optical Variables Measurements
URL	查看原文
PubMed ID	3849942
SCOPUSEID	2-s2.0-85186263689
通讯作者地址	[Quan, Yun-Yun]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China ; [Ye, Xiaoxia]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China ; [Xie, Longteng]The Affiliated Xiangshan Hospital of Wenzhou Medical University,Xiangshan First People's Hospital Medical and Health Group,Xiangshan,315700,China
Scopus学科分类	Analytical Chemistry;Environmental Chemistry;Biochemistry;Spectroscopy
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210308
专题	药学院（分析测试中心）其他_附属象山医院（象山县第一人民医院）其他_附属萧山医院（杭州市萧山区第一人民医院）
通讯作者	Ye, Xiaoxia; Xie, Longteng; Quan, Yun-Yun
作者单位	1.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 2.The Affiliated Xiangshan Hospital of Wenzhou Medical University,Xiangshan First People's Hospital Medical and Health Group,Xiangshan,315700,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）; 附属萧山医院（杭州市萧山区第一人民医院）; 附属象山医院（象山县第一人民医院）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Huang, Zu-Sheng,Zhang, Wenxuan,Liang, Manshan,et al. A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice[J]. Analytica chimica acta,2024,1299.
APA	Huang, Zu-Sheng., Zhang, Wenxuan., Liang, Manshan., Wang, Shihua., Zhang, Zhongda., ... & Quan, Yun-Yun. (2024). A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice. Analytica chimica acta, 1299.
MLA	Huang, Zu-Sheng,et al."A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice".Analytica chimica acta 1299(2024).