YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS

doi:10.1016/j.celrep.2024.113947

科研成果详情

题名	YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS
作者	Wang, Jing 1,2; Zhou, Yao 1; Zhang, Meng 1; Wu, Yujiao 1; Wu, Qun 1; Su, Wen 1; Xu, Min 2; Wu, Jinhong 2; Zhang, Min 2; Shuai, Jianwei 4,5; Tang, Wei 3; Lv, Jiajia 1; Wu, Min 4,5; Xia, Zhenwei 1
发表日期	2024-03-26
发表期刊	Cell reports 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	CP: Immunology CP: Molecular biology N6-methyladenosine YTHDF1 allergic airway inflammation liquid-liquid phase separation translation
其他关键词	N-6-METHYLADENOSINE RNA ; STRESS ; METHYLATION ; TRANSLATION ; BINDING
摘要	N6-methyladenosine (m6A) modification has been implicated in many cell processes and diseases. YTHDF1, a translation-facilitating m6A reader, has not been previously shown to be related to allergic airway inflammation. Here, we report that YTHDF1 is highly expressed in allergic airway epithelial cells and asthmatic patients and that it influences proinflammatory responses. CLOCK, a subunit of the circadian clock pathway, is the direct target of YTHDF1. YTHDF1 augments CLOCK translation in an m6A-dependent manner. Allergens enhance the liquid-liquid phase separation (LLPS) of YTHDF1 and drive the formation of a complex comprising dimeric YTHDF1 and CLOCK mRNA, which is distributed to stress granules. Moreover, YTHDF1 strongly activates NLRP3 inflammasome production and interleukin-1β secretion leading to airway inflammatory responses, but these phenotypes are abolished by deleting CLOCK. These findings demonstrate that YTHDF1 is an important regulator of asthmatic airway inflammation, suggesting a potential therapeutic target for allergic airway inflammation.
资助项目	National Natural Science Foundation of China[91542202,82300037,81900015,82170035,81970021];National Key Research Project of MOST[2023YFA0915000];
出版者	CELL PRESS
ISSN	2211-1247
卷号	43 期号:3
DOI	10.1016/j.celrep.2024.113947
页数	21
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:001210296000001
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	3849222
SCOPUSEID	2-s2.0-85187978015
通讯作者地址	[Xia, Zhenwei]Department of Pediatrics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China ; [Lv, Jiajia]Department of Pediatrics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China ; [Wu, Min]Joint Research Centre on Medicine,The Affiliated Xiangshan Hospital of Wenzhou Medical University,Ningbo,China ; [Tang, Wei]Department of Pulmonary and Critical Care Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all)
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210296
专题	其他_附属萧山医院（杭州市萧山区第一人民医院）其他_附属象山医院（象山县第一人民医院）
通讯作者	Tang, Wei; Lv, Jiajia; Wu, Min; Xia, Zhenwei
作者单位	1.Department of Pediatrics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China; 2.Department of Surgery,Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai,China; 3.Department of Pulmonary and Critical Care Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China; 4.Joint Research Centre on Medicine,The Affiliated Xiangshan Hospital of Wenzhou Medical University,Ningbo,China; 5.Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,China
通讯作者单位	附属萧山医院（杭州市萧山区第一人民医院）; 附属象山医院（象山县第一人民医院）
推荐引用方式 GB/T 7714	Wang, Jing,Zhou, Yao,Zhang, Meng,et al. YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS[J]. Cell reports,2024,43(3).
APA	Wang, Jing., Zhou, Yao., Zhang, Meng., Wu, Yujiao., Wu, Qun., ... & Xia, Zhenwei. (2024). YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS. Cell reports, 43(3).
MLA	Wang, Jing,et al."YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS".Cell reports 43.3(2024).