Knockdown of ribosome RNA processing protein 15 suppresses migration of hepatocellular carcinoma through inhibiting PATZ1-associated LAMC2/FAK pathway

doi:10.1186/s12885-024-12065-4

科研成果详情

题名	Knockdown of ribosome RNA processing protein 15 suppresses migration of hepatocellular carcinoma through inhibiting PATZ1-associated LAMC2/FAK pathway
作者	Pan, Tongtong 2; Li, Jinhai3; Zhang, Ouyang 1; Zhu, Yuqin1; Zhou, Hongfei2; Ma, Mengchen1; Yu, Yanwen1; Lyu, Jiaojian 4; Chen, Yongping2; Xu, Liang1
发表日期	2024-03-12
发表期刊	BMC Cancer 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Focal adhesion kinase Hepatocellular carcinoma migration LAMC2 Ribosome RNA processing protein 15
其他关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; NF-KAPPA-B ; TUMOR-GROWTH ; EPIDERMAL-GROWTH ; INTEGRIN ; METASTASIS ; FAK ; LAMININ ; PROGRESSION ; EXPRESSION
摘要	Ribosomal RNA processing protein 15 (RRP15) has been found to regulate the progression of hepatocellular carcinoma (HCC). Nevertheless, the extent to which it contributes to the spread of HCC cells remains uncertain. Thus, the objective of this research was to assess the biological function of RRP15 in the migration of HCC., The expression of RRP15 in HCC tissue microarray (TMA), tumor tissues and cell lines were determined. In vitro, the effects of RRP15 knockdown on the migration, invasion and adhesion ability of HCC cells were assessed by wound healing assay, transwell and adhesion assay, respectively. The effect of RRP15 knockdown on HCC migration was also evaluated in vivo in a mouse model., Bioinformatics analysis showed that high expression of RRP15 was significantly associated with low survival rate of HCC. The expression level of RRP15 was strikingly upregulated in HCC tissues and cell lines compared with the corresponding controls, and TMA data also indicated that RRP15 was a pivotal prognostic factor for HCC. RRP15 knockdown in HCC cells reduced epithelial-to-mesenchymal transition (EMT) and inhibited migration in vitro and in vivo, independent of P53 expression. Mechanistically, blockade of RRP15 reduced the protein level of the transcription factor POZ/BTB and AT hook containing zinc finger 1 (PATZ1), resulting in decreased expression of the downstream genes encoding laminin 5 subunits, LAMC2 and LAMB3, eventually suppressing the integrin β4 (ITGB4)/focal adhesion kinase (FAK)/nuclear factor κB kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway., RRP15 promotes HCC migration by activating the LAMC2/ITGB4/FAK pathway, providing a new target for future HCC treatment.
资助项目	Natural Science Foundation of Zhejiang Province[LD21H030002];Natural Science Foundation of Zhejiang Province[LY21H070004];National Natural Science Foundation of China[81900778]
出版者	BMC
ISSN	1471-2407
EISSN	1471-2407
卷号	24 期号:1
DOI	10.1186/s12885-024-12065-4
页数	13
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:001183951000002
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	3847574
SCOPUSEID	2-s2.0-85187502362
通讯作者地址	[Xu, Liang]Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China ; [Chen, Yongping]Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
Scopus学科分类	Oncology;Genetics;Cancer Research
SCOPUS_ID	SCOPUS_ID:85187502362
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210264
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院其他_附属第三医院（瑞安市人民医院）
通讯作者	Chen, Yongping; Xu, Liang
作者单位	1.Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 3.Department of Liver and Gall Surgery,The Third Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325200,China; 4.Department of Infectious Diseases,Lishui People’s Hospital,Zhejiang,Lishui,323000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Pan, Tongtong,Li, Jinhai,Zhang, Ouyang,et al. Knockdown of ribosome RNA processing protein 15 suppresses migration of hepatocellular carcinoma through inhibiting PATZ1-associated LAMC2/FAK pathway[J]. BMC Cancer,2024,24(1).
APA	Pan, Tongtong., Li, Jinhai., Zhang, Ouyang., Zhu, Yuqin., Zhou, Hongfei., ... & Xu, Liang. (2024). Knockdown of ribosome RNA processing protein 15 suppresses migration of hepatocellular carcinoma through inhibiting PATZ1-associated LAMC2/FAK pathway. BMC Cancer, 24(1).
MLA	Pan, Tongtong,et al."Knockdown of ribosome RNA processing protein 15 suppresses migration of hepatocellular carcinoma through inhibiting PATZ1-associated LAMC2/FAK pathway".BMC Cancer 24.1(2024).