A newly developed UPLC-MS/MS method for simultaneous quantitative analysis of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-acetylharpagide derived from Ajuga plants in mice blood and the in vivo pharmacokinetics

doi:10.1080/03639045.2024.2328731

科研成果详情

题名	A newly developed UPLC-MS/MS method for simultaneous quantitative analysis of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-acetylharpagide derived from Ajuga plants in mice blood and the in vivo pharmacokinetics
作者	Shen, Xiuwei 1; Chen, Chen2; Wen, Congcong 3; Yu, Shuaishuai4; Liu, Huamin3; Gao, Xiaomin 5; Chen, Lianguo2
发表日期	2024-04-02
发表期刊	DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	Ajuforrestin A ajuforrestin B ajugamacrin 8-O-acetylharpagide pharmacokinetics UPLC-MS/MS
其他关键词	NEOCLERODANE DITERPENOIDS ; IRIDOIDS ; GLYCOSIDE ; L.
摘要	Objective To develop a sensitive and fast detection method via ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to assess the concentration of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-Acetylharpagide primarily derived from Ajuga plants in mice blood and their pharmacokinetics. Methods Single protein precipitation with high-proportioned acetonitrile is chosen for sample clean-up. The UPLC HSS T3 (2.1 mm x 100 mm, 1.8 mu m) column with a mobile phase in gradient elution mode at the flow rate of 0.4 mL/min was used for sample separation. Acetonitrile was selected as the organic phase solution and water containing 0.1% formic acid was chosen as the aqueous solution. A tandem mass spectrometer containing an electrospray ionization (ESI) source in the positive ionization mode was used to detect four compounds via multiple reaction monitoring (MRM). Results The calibration curves (5-1000 ng/mL) of four compounds were linear with correlation coefficients > 0.997. The matrix effects, accuracy, precision, and recovery were all within permissible scope. Conclusions In this approach, the corresponding pharmacokinetic parameters were successfully clarified in mouse for the first time, which provided a theoretical basis for the improvement of the standard of Ajuga plants and the safety of clinical medication. Furthermore, this method may provide the UPLC-MS/MS evidence for the differentiation of the main close relative varieties of genus Ajuga according to these plants contain different mixtures of the four marker compounds.
资助项目	Wenzhou Medical University
出版者	TAYLOR & FRANCIS LTD
ISSN	0363-9045
EISSN	1520-5762
卷号	50 期号:4 页码:354-362
DOI	10.1080/03639045.2024.2328731
页数	9
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:001185237100001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2024-03
URL	查看原文
PubMed ID	38456836
SCOPUSEID	2-s2.0-85188312789
通讯作者地址	[Chen, Lianguo]Department of Pharmacy,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China ; [Gao, Xiaomin]Department of Urology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Pharmacology;Pharmaceutical Science;Drug Discovery;Organic Chemistry
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210037
专题	其他_附属第三医院（瑞安市人民医院）检验医学院（生命科学学院、生物学实验教学中心）附属第一医院实验动物中心
通讯作者	Gao, Xiaomin; Chen, Lianguo
作者单位	1.Department of Pharmacy,The Third Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Pharmacy,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.Laboratory Animal Center,Wenzhou Medical University,Wenzhou,China; 4.Department of Biology,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou,China; 5.Department of Urological Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	其他_附属第三医院（瑞安市人民医院）
通讯作者单位	附属第一医院
第一作者的第一单位	其他_附属第三医院（瑞安市人民医院）
推荐引用方式 GB/T 7714	Shen, Xiuwei,Chen, Chen,Wen, Congcong,et al. A newly developed UPLC-MS/MS method for simultaneous quantitative analysis of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-acetylharpagide derived from Ajuga plants in mice blood and the in vivo pharmacokinetics[J]. DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY,2024,50(4):354-362.
APA	Shen, Xiuwei., Chen, Chen., Wen, Congcong., Yu, Shuaishuai., Liu, Huamin., ... & Chen, Lianguo. (2024). A newly developed UPLC-MS/MS method for simultaneous quantitative analysis of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-acetylharpagide derived from Ajuga plants in mice blood and the in vivo pharmacokinetics. DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 50(4), 354-362.
MLA	Shen, Xiuwei,et al."A newly developed UPLC-MS/MS method for simultaneous quantitative analysis of ajuforrestin A, ajuforrestin B, ajugamacrin and 8-O-acetylharpagide derived from Ajuga plants in mice blood and the in vivo pharmacokinetics".DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY 50.4(2024):354-362.