Growth differentiation factor 11 suppresses intrahepatic inflammation via restricting NLRP3 inflammasome activation in LPS-induced liver injury

doi:10.14715/cmb/2023.69.13.37

科研成果详情

题名	Growth differentiation factor 11 suppresses intrahepatic inflammation via restricting NLRP3 inflammasome activation in LPS-induced liver injury
作者	Huang, Hui 1; Wu, Shengyi 1; Yan, Yawei2
发表日期	2023
发表期刊	CELLULAR AND MOLECULAR BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article ; Journal Article
关键词	GDF11 infectious acute liver injury NLRP3 inflammasome inflammatory response
其他关键词	SEPSIS
摘要	Growth differentiation factor 11 (GDF11) is reported as a member of TGF-beta superfamily, which plays a key negative role in various tissue inflammation. However, the specific effect of GDF11 on infectious acute liver injury remains unknown. The current study is designed to certify the role of GDF11 both in LPS-induced RAW 264.7 cell line and rodent model of acute liver injury (ALI) and further investigate its molecular mechanism of inflammatory regulation. In vitro, LPS was used to stimulate the inflammatory activation of RAW 264.7 cells and then recombinant GDF11 (rGDF11) was used to treat the cells. In vivo, we injected LPS and rGDF11 in abdomen of mouse. The inflammatory indexes, GDF11 level, NLRP3 level, liver tissue injury, and liver function were examined using qRT-PCR, western blot, ELISA, IHC, IF and HE staining, respectively. Supplement of GDF11 protected the histology and function of liver tissue in LPS-induced ALI mice, in which the level of AST, ALT and TBiL associated with tissue damage were reduced after ALI. Moreover, increased GDF11 in RAW 264.7 cells and ALI mice reduced the expressions of COX-2, TNF-alpha, IL-1 beta, and IL-6 via inhibiting NLRP3 inflammasome activation, suggesting the anti-inflammatory role of GDF11 in ALI. Besides, owing to the protective role of GDF11, the apoptotic degree in liver after LPS insult was attenuated, such as the reduced c-caspase-3 and annexin-V expressions. The results indicate that overexpression of GDF11 plays an antagonistic role in LPS-induced inflammatory response after ALI. Therefore, GDF11 may become a promising target for preventing infectious acute liver injury. Copyright: (c) 2023 by the C.M.B. Association. All rights reserved.
出版者	C M B ASSOC
ISSN	0145-5680
EISSN	1165-158X
卷号	69 期号:13 页码:250-255
DOI	10.14715/cmb/2023.69.13.37
页数	6
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:001164878200037
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	38158658
SCOPUSEID	2-s2.0-85181414301
通讯作者地址	[Yan, Yawei]Department of Biopharmaceuticals,College of Pharmacy,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Biochemistry;Molecular Biology;Cell Biology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/209908
专题	药学院（分析测试中心）_生物制药系
通讯作者	Yan, Yawei
作者单位	1.Department of Pharmacy,Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou,China; 2.Department of Biopharmaceuticals,College of Pharmacy,Wenzhou Medical University,Wenzhou,China
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Huang, Hui,Wu, Shengyi,Yan, Yawei. Growth differentiation factor 11 suppresses intrahepatic inflammation via restricting NLRP3 inflammasome activation in LPS-induced liver injury[J]. CELLULAR AND MOLECULAR BIOLOGY,2023,69(13):250-255.
APA	Huang, Hui, Wu, Shengyi, & Yan, Yawei. (2023). Growth differentiation factor 11 suppresses intrahepatic inflammation via restricting NLRP3 inflammasome activation in LPS-induced liver injury. CELLULAR AND MOLECULAR BIOLOGY, 69(13), 250-255.
MLA	Huang, Hui,et al."Growth differentiation factor 11 suppresses intrahepatic inflammation via restricting NLRP3 inflammasome activation in LPS-induced liver injury".CELLULAR AND MOLECULAR BIOLOGY 69.13(2023):250-255.