SIRT1 Activation Reduces LPS-Induced Human Middle Ear Epithelial Cell Injury via Promoting Autophagy

doi:10.23812/j.biol.regul.homeost.agents.20243802.107

科研成果详情

题名	SIRT1 Activation Reduces LPS-Induced Human Middle Ear Epithelial Cell Injury via Promoting Autophagy
作者	Zheng, Bo1,2; Zheng, Lei1,2; Liu, Fanli1,2
发表日期	2024-02
发表期刊	JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	otitis media SIRT1 inflammatory response autophagy human middle ear epithelial cells
其他关键词	ACUTE OTITIS-MEDIA ; COCHLEAR HAIR CELL ; OXIDATIVE STRESS ; KAPPA-B ; INFLAMMATION
摘要	Background: Otitis media (OM) is a prevalent pervasive illness among the pediatric population. Research on sirtuin 1 (SIRT1), a deacetylase sensitive to nicotinamide adenine dinucleotide (NAD(+)), has increasingly identified its pivotal role in various inflammatory conditions. Our study aims to elucidate the role of SIRT1 in inflammatory injury in OM and its potential action mechanism. Methods: Human middle ear epithelial cells (HMEECs) were stimulated with lipopolysaccharide (LPS) to establish in vitro models of OM. Western blot was used to assess SIRT1 expression. Following the addition of SIRT1 activator SRT1720, with or without the autophagy inhibitor 3-Methyladenine (3-MA), the Cell Counting Kit-8 (CCK-8) assay was used to evaluate cell viability. Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay was used to assess cell apoptotic levels. Enzyme-linked immunosorbent assay (ELISA) was used to estimate inflammatory levels and dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining was used to estimate reactive oxygen species (ROS) activity. Western blot was used to analyze the content of apoptosis-, inflammatory response-, autophagy-, and nuclear factor-kappaB (NF-kappa B) signaling-associated proteins. Results: SIRT1 expression was attenuated in LPS-treated HMEECs (p < 0.05). SRT1720 pretreatment significantly enhanced cell viability (p < 0.05), attenuated apoptosis (p < 0.05), suppressed the inflammatory response (p < 0.05), and reduced ROS production (p < 0.05). Additionally, it inhibited NF-kappa B signaling (p < 0.05) while inducing autophagy (p < 0.05) in HMEECs exposed to LPS in a concentration-dependent manner. Furthermore, 3-Methyladenine (3-MA) partially counteracted the suppressive effects of SRT1720 in LPS-induced viability loss, apoptosis (p < 0.05), inflammatory response (p < 0.05), NF-kappa B signaling activation (p < 0.05), and ROS generation (p < 0.05) in HMEECs. Conclusions: In summary, the activation of SIRT1 may mitigate the inflammatory response in LPS-induced HMEECs through the modulation of apoptosis, inflammatory signaling, and autophagic processes.
出版者	BIOLIFE SAS
ISSN	0393-974X
EISSN	1724-6083
卷号	38 期号:2 页码:1341-1350
DOI	10.23812/j.biol.regul.homeost.agents.20243802.107
页数	10
WOS类目	Endocrinology & Metabolism ; Immunology ; Medicine, Research & Experimental ; Physiology
WOS研究方向	Endocrinology & Metabolism ; Immunology ; Research & Experimental Medicine ; Physiology
WOS记录号	WOS:001159366700001
收录类别	SCIE
通讯作者地址	[Liu, Fanli]Wenzhou Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Wenzhou 325027, Zhejiang, Peoples R China. ; [Liu, Fanli]Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/209890
专题	附属第二医院_耳鼻咽喉科第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Liu, Fanli
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China
第一作者单位	附属第二医院_耳鼻咽喉科; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	附属第二医院_耳鼻咽喉科; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第二医院_耳鼻咽喉科
推荐引用方式 GB/T 7714	Zheng, Bo,Zheng, Lei,Liu, Fanli. SIRT1 Activation Reduces LPS-Induced Human Middle Ear Epithelial Cell Injury via Promoting Autophagy[J]. JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,2024,38(2):1341-1350.
APA	Zheng, Bo, Zheng, Lei, & Liu, Fanli. (2024). SIRT1 Activation Reduces LPS-Induced Human Middle Ear Epithelial Cell Injury via Promoting Autophagy. JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS, 38(2), 1341-1350.
MLA	Zheng, Bo,et al."SIRT1 Activation Reduces LPS-Induced Human Middle Ear Epithelial Cell Injury via Promoting Autophagy".JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 38.2(2024):1341-1350.