Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells

doi:10.2147/IJN.S129375

科研成果详情

题名	Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells
作者	Wei, Limin1,2; Wang, Jianfeng1,2; Chen, Aijie 1; Liu, Jia 1; Feng, Xiaoli 1; Shao, Longquan 1
发表日期	2017-12-31
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	PINK1/parkin mitophagy autophagy zinc oxide nanoparticles neurotoxicity
其他关键词	ZINC-OXIDE NANOPARTICLES ; MITOCHONDRIAL DYSFUNCTION ; TITANIUM-DIOXIDE ; BRAIN MICROGLIA ; EXPOSURE ; PATHWAY ; PARKIN ; HOMEOSTASIS ; APOPTOSIS ; AUTOPHAGY
摘要	With the increasing application of zinc oxide nanoparticles (ZnO NPs) in biological materials, the neurotoxicity caused by these particles has raised serious concerns. However, the underlying molecular mechanisms of the toxic effect of ZnO NPs on brain cells remain unclear. Mitochondrial damage has been reported to be a factor in the toxicity of ZnO NPs. PINK1/parkin-mediated mitophagy is a newly emerging additional function of autophagy that selectively degrades impaired mitochondria. Here, a PINK1 gene knockdown BV-2 cell model was established to determine whether PINK1/parkin-mediated mitophagy was involved in ZnO NP-induced toxicity in BV-2 cells. The expression of total parkin, mito-parkin, cyto-parkin, and PINK1 both in wild type and PINK1(-/-) BV-2 cells was evaluated using Western blot analysis after the cells were exposed to 10 mu g/mL of 50 nm ZnO NPs for 2, 4, 8, 12, and 24 h. The findings suggested that the downregulation of PINK1 resulted in a significant reduction in the survival rate after ZnO NP exposure compared with that of control cells. ZnO NPs were found to induce the transportation of parkin from the cytoplasm to the mitochondria, implying the involvement of mitophagy in ZnO NP-induced toxicity. The deletion of the PINK1 gene inhibited the recruitment of parkin to the mitochondria, causing failure of the cell to trigger mitophagy. The present study demonstrated that apart from autophagy, PINK1/parkin-mediated mitophagy plays a protective role in ZnO NP-induced cytotoxicity.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY16H140003, LY17H140008]; Wenzhou Municipal Science and Technology Bureau Foundation of China [Y20150070]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51672122, 81550011, 51172283]
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1178-2013
EISSN	1178-2013
卷号	12 页码:1891-1903
DOI	10.2147/IJN.S129375
页数	13
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS记录号	WOS:000395712900002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28331313
PMC记录号	PMC5352242
SCOPUSEID	2-s2.0-85015095318
通讯作者地址	[Shao, Longquan]Department of Stomatology,Nanfang Hospital,Southern Medical University,1838 North Guangzhoudadao,Guangzhou,510515,China
Scopus学科分类	Biophysics;Bioengineering;Biomaterials;Pharmaceutical Science;Drug Discovery;Organic Chemistry
TOP期刊	TOP期刊
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2096
专题	口腔医学院、附属口腔医院_儿童口腔
通讯作者	Shao, Longquan
作者单位	1.Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou,China; 2.Department of Pediatric Dentistry,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China
第一作者单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Wei, Limin,Wang, Jianfeng,Chen, Aijie,et al. Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12:1891-1903.
APA	Wei, Limin, Wang, Jianfeng, Chen, Aijie, Liu, Jia, Feng, Xiaoli, & Shao, Longquan. (2017). Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells. INTERNATIONAL JOURNAL OF NANOMEDICINE, 12, 1891-1903.
MLA	Wei, Limin,et al."Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells".INTERNATIONAL JOURNAL OF NANOMEDICINE 12(2017):1891-1903.