The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia

doi:10.1016/j.jtcme.2024.01.001

科研成果详情

题名	The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia
作者	Feng, Yuming 1,2; Zhao, Shanmeizi 2,3; Ju, Rong 4; Li, Jianbo 5; Pan, Chengshuang6; Tang, Qinglai 7; Zou, Zhichuan 2; Jing, Jun 2; Xu, Yao 8; Ding, Hualong 2; Ma, Jinzhao 2; Li, Zhou 9; Tang, Ting 2; Shao, Yong 2; Chen, Li 2; Huang, Xuefeng6; Wang, Xiaohong 5; Yao, Bing 1,2,3
发表日期	2024
发表期刊	Journal of Traditional and Complementary Medicine; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Glucose-6-phosphate Guilingji Metabolomics Oligo-asthenoteratozoospermia Traditional Chinese medicine
其他关键词	SERTOLI-CELLS ; INHIBIN B ; SPERMATOGENESIS ; GLUCOSE-6-PHOSPHATE
摘要	Introduction: Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there has been little evidence-based clinical research on the use of Guilingji to treat idiopathic oligo-asthenoteratozoospermia (OAT), and the therapeutic mechanism from a metabolic perspective needs to be investigated further. Methods: This was a multicenter, double-blind, randomized controlled clinical study of 240 patients with idiopathic OAT recruited from four hospitals between January 2020 and January 2022. Patients were randomly assigned in a 1꞉1 ratio to receive oral Guilingji capsules or placebo for 12 weeks. The total progressive motile sperm count (TPMSC) was considered the primary outcome, and the other sperm parameters, seminal plasma parameters and serum hormones were considered the secondary outcome. A nontargeted metabolomics analysis of serum from OAT patients before and after Guilingji administration was performed by HPLC–MS to identify key metabolites. Furthermore, we used a rat model to show spermatogenesis phenotypes to validate the effect of the key metabolites screened from the patients. Results: At weeks 4, 8 and 12, TPMSC and other sperm parameters were significantly improved in the Guilingji group compared with the placebo group (P < 0.05 for all comparisons). At week 4, superoxide dismutase (SOD) and acrosomal enzyme activity of seminal plasma were significantly elevated in the Guilingji group compared with the placebo group, while reactive oxygen species (ROS) levels were significantly reduced (P < 0.05). Lactate dehydrogenase-X (LDHX) levels appeared to be significantly increased after 12 weeks continuous medication compared with Placebo group (P = 0.032). The metabolomics analysis of serum from OAT patients before and after Guilingji administration showed that the glucose-6-phosphate (G6P) concentration in patients' serum was significantly elevated after Guilingji treatment. Compared to the control, when Kidney-Yang deficiency model rats were treated with Guilingji or its key intermediate metabolite G6P, their sperm concentration and spermatozoic activity were improved similarly, and their structural damage of rat's testicular and epididymal tissues were recovered. Conclusion: This study provided valuable clinical evidence for the utility of Guilingji as a treatment for OAT. These findings thus demonstrate that G6P is involved in the therapeutic mechanism of Guilingji in OAT treatment based on clinical and rat intervention studies.
资助项目	Annual Foundation of Nanjing Jinling Hospital[22JCYYYB4];Jiangsu Provincial Medical Key Discipline Cultivation Unit[JSDW202215];National Natural Science Foundation of China[81701431,81971373,81973965,82001618];National Key Research and Development Program of China[2018YFC1003800,2018YFC1004700];
出版者	National Taiwan University
ISSN	2225-4110
卷号	14 期号:4 页码:403-413
DOI	10.1016/j.jtcme.2024.01.001
页数	11
WOS类目	Integrative & Complementary Medicine
WOS研究方向	Integrative & Complementary Medicine
WOS记录号	WOS:001261226800001
收录类别	SCOPUS ; SCIE ; PUBMED
URL	查看原文
PubMed ID	39035689
SCOPUSEID	2-s2.0-85182353160
通讯作者地址	[Yao, Bing]Department of Reproductive Medicine,Jinling Hospital,305 Zhongshan East Road,Nanjing,210002,China ; [Chen, Li]Department of Reproductive Medicine,Jinling Hospital,Affiliated Hospital of Medical School,Nanjing University,Jiangsu,Nanjing,210002,China ; [Huang, Xuefeng]Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China ; [Wang, Xiaohong]Center for Reproductive Medicine of Obstetrics and Gynecology,Tangdu Hospital,Air Force Medical University,Shaanxi,Xi'an,710032,China
Scopus学科分类	Complementary and Alternative Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/208005
专题	附属第一医院_生殖医学中心
通讯作者	Chen, Li; Huang, Xuefeng; Wang, Xiaohong; Yao, Bing
作者单位	1.The First School of Clinical Medicine,Southern Medical University,Guangdong,Guangzhou,510515,China; 2.Department of Reproductive Medicine,Jinling Hospital,Affiliated Hospital of Medical School,Nanjing University,Jiangsu,Nanjing,210002,China; 3.Jiangsu Key Laboratory for Molecular and Medical Biotechnology,College of Life Sciences,Nanjing Normal University,Jiangsu,Nanjing,210023,China; 4.Department of Obstetrics and Gynecology,The Affiliated Jiangning Hospital with Nanjing Medical University,Jiangsu,Nanjing,211100,China; 5.Center for Reproductive Medicine of Obstetrics and Gynecology,Tangdu Hospital,Air Force Medical University,Shaanxi,Xi'an,710032,China; 6.Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 7.Department of Urology,The Affiliated Jiangning Hospital of Nanjing Medical University,Jiangsu,Nanjing,211100,China; 8.State Key Laboratory of Reproductive Medicine,Nanjing Medical University,Jiangsu,Nanjing,211116,China; 9.Department of Reproductive Medicine,Nanjing Jinling Hospital,School of Medicine,Jiangsu University,Jiangsu,Zhenjiang,212000,China
通讯作者单位	附属第一医院_生殖医学中心
推荐引用方式 GB/T 7714	Feng, Yuming,Zhao, Shanmeizi,Ju, Rong,et al. The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia[J]. Journal of Traditional and Complementary Medicine;,2024,14(4):403-413.
APA	Feng, Yuming., Zhao, Shanmeizi., Ju, Rong., Li, Jianbo., Pan, Chengshuang., ... & Yao, Bing. (2024). The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia. Journal of Traditional and Complementary Medicine;, 14(4), 403-413.
MLA	Feng, Yuming,et al."The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia".Journal of Traditional and Complementary Medicine; 14.4(2024):403-413.