Graphene oxide quantum dots (GOQDs) induce behavioral disorders via the disturbance of kynurenine pathway in zebrafish larvae

doi:10.1016/j.cbpc.2024.109865

科研成果详情

题名	Graphene oxide quantum dots (GOQDs) induce behavioral disorders via the disturbance of kynurenine pathway in zebrafish larvae
作者	Deng, Shun 1; Zhang, Enming 2; Zhao, Yunyang3; Guo, Hao 2; Luo, Lan 4; Yi, Song 1; Zheng, Bingxin 1; Mu, Xiaoyuan 1; Yuan, Kezhu 1; Deng, Xuangen 1; Rong, Haibo 1; Ma, Yanbo 4; Bian, Wanping 4; Shen, Hai 1
发表日期	2024-05
发表期刊	Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	GOQDs Kynurenine pathway Metabolomics Thigmotaxis Zebrafish
其他关键词	PERTURBATION ; TOXICITY
摘要	The emergence of graphene quantum dots (GQDs) expands the use of graphene derivatives in nanomedicine for its direct therapeutic applications in treating neurodegeneration, inflammation, metabolic dysfunction, and among others. Nevertheless, the biosafety assessment of GQDs remains deficient mostly because of the diverse surface characteristics of the nanoparticles. Our prior work demonstrated that GQDs can induce strong thigmotactic effects in zebrafish larvae over a wide range of concentrations, yet the underlying metabolic mechanisms remain largely unknown. In this study, we conducted a further exploration about graphene oxide quantum dots (GOQDs) for its potential neurotoxic effect on the behaviors of zebrafish larvae by combining neurotransmitter-targeted metabolomics with locomotion analysis. After continuous exposure to a concentration gradient of GOQDs (12.5 - 25 - 50 - 100 - 200 μg/mL) for 7 days, the thigmotactic activities of zebrafish larvae were observed across all exposure concentrations relative to the control group, while the basal locomotor activities, including distance moved and average velocity, were significantly changed by low concentrations of GOQDs. Targeted metabolomics was performed using zebrafish larvae at 7 days post-fertilization (dpf) that were exposed to 12.5 and 200 μg/mL, both of which were found to perturb the kynurenine pathway by regulating the levels of kynurenine, 3-hydroxyanthranilic acid (3-HAA), and quinolinic acid (QA). Furthermore, the thigmotaxis of larval fish induced by GOQDs during exposure could be counteracted by supplementing Ro-61-8048, an agonist acting on kynurenine 3-monooxygenase (KMO). In conclusion, our study establishes the involvement of the kynurenine pathway in GOQDs-induced thigmotaxis, which is independent of the transcriptional modulation of glutamate receptor families.
资助项目	Central Government Funds of Guiding Local Scientific and Technological Development for Sichuan Province [2021ZYD0068]
出版者	ELSEVIER SCIENCE INC
ISSN	1532-0456
EISSN	1878-1659
卷号	279
DOI	10.1016/j.cbpc.2024.109865
页数	8
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS记录号	WOS:001210991800001
收录类别	PUBMED ; SCOPUS ; SCIE
在线发表日期	2024-03
URL	查看原文
PubMed ID	38403007
SCOPUSEID	2-s2.0-85187016419
通讯作者地址	[Deng, Shun]Sichuan Provincial Orthopedic Hospital,Chengdu,610041,China
Scopus学科分类	Biochemistry;Physiology;Aquatic Science;Animal Science and Zoology;Toxicology;Cell Biology;Health, Toxicology and Mutagenesis
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/207736
专题	附属第二医院_科研中心
通讯作者	Deng, Shun; Shen, Hai
作者单位	1.Sichuan Provincial Orthopedic Hospital,Chengdu,610041,China; 2.School of Sports Medicine and Physical Therapy,Beijing Sport University,Beijing,100084,China; 3.Scientific Research Center,Wenzhou Medical University,Wenzhou,325035,China; 4.Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Science,Chongqing,400714,China
推荐引用方式 GB/T 7714	Deng, Shun,Zhang, Enming,Zhao, Yunyang,et al. Graphene oxide quantum dots (GOQDs) induce behavioral disorders via the disturbance of kynurenine pathway in zebrafish larvae[J]. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,2024,279.
APA	Deng, Shun., Zhang, Enming., Zhao, Yunyang., Guo, Hao., Luo, Lan., ... & Shen, Hai. (2024). Graphene oxide quantum dots (GOQDs) induce behavioral disorders via the disturbance of kynurenine pathway in zebrafish larvae. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 279.
MLA	Deng, Shun,et al."Graphene oxide quantum dots (GOQDs) induce behavioral disorders via the disturbance of kynurenine pathway in zebrafish larvae".Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 279(2024).