Liensinine inhibits progression of intrahepatic cholangiocarcinoma by regulating TGF-β1 /P-smad3 signaling through HIF-1a

doi:10.1002/mc.23687

科研成果详情

题名	Liensinine inhibits progression of intrahepatic cholangiocarcinoma by regulating TGF-β1 /P-smad3 signaling through HIF-1a
作者	Zhu, Xuewen1,2; Bao, Wenming1,2; Xie, Xiaozai1,2; Chen, Bo 1,2; Li, Rizhao1,2; Zhao, Jungang1,2; Wu, Lijun 1,2; Yu, Zhengping1,2; Li, Shi 3; Zhu, Qiandong1,2,5; Chen, Gang1,2,4,5; Li, Jiacheng1,2,5
发表日期	2024-01-30
发表期刊	MOLECULAR CARCINOGENESIS 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	cholangiocarcinoma epithelial-mesenchymal transition HIF-1a liensinine
其他关键词	CANCER CELLS ; GEMCITABINE RESISTANCE ; HEPATOCELLULAR-CARCINOMA ; HIF-1-ALPHA ; HYPOXIA ; DIAGNOSIS ; CHEMOTHERAPY ; METASTASIS ; MANAGEMENT
摘要	Intrahepatic cholangiocarcinoma (ICC) is a high-grade malignant digestive system tumor with an insidious onset and unfavorable prognosis. Liensinine, a small molecule derived from plants, has been proven to have significant tumor suppressor activity in other cancers. However, there are no reports on whether liensinine can inhibit the proliferation or metastasis of ICC. This study aimed to explore the tumor-suppressive activity of liensinine in ICC and its underlying mechanisms. The phenotypic changes in ICC cells were monitored in vitro using cell function tests. Western blot and immunofluorescence analyses verified the efficacy of liensinine. Tumor-bearing nude mice were used to explore the effect of liensinine on tumors and its toxicity and side effects in vivo. Liensinine suppressed ICC cell proliferation and arrested the cell cycle at the G1 phase. The epithelial-mesenchymal transition (EMT) of ICC cells was also inhibited, thereby restraining their invasion and migration of tumor cells. In addition, this study found that the potential mechanism of liensinine inhibiting EMT may be via suppression of the TGF-beta 1/P-smad3 signaling pathway through hypoxia-inducible factor 1 alpha (HIF-1a). In vivo experiments showed that liensinine inhibited the growth of Hucc-T1 transplanted tumors in nude mice. Liensinine restrained the proliferation of ICC cells and suppressed EMT in ICC via the HIF-1a-mediated TGF-beta 1/P-smad3 signaling pathway.
资助项目	Science and Technology Plan Project of Wenzhou; National Natural Science Foundation of China [82072685]; [Y2020153]
出版者	WILEY
ISSN	0899-1987
EISSN	1098-2744
DOI	10.1002/mc.23687
页数	13
WOS类目	Biochemistry & Molecular Biology ; Oncology
WOS研究方向	Biochemistry & Molecular Biology ; Oncology
WOS记录号	WOS:001152250000001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	38289159
SCOPUSEID	2-s2.0-85183886308
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/207333
专题	附属第一医院_肝胆外科附属第一医院附属第一医院_泌尿外科
通讯作者	Zhu, Qiandong; Chen, Gang; Li, Jiacheng
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Wenzhou, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Diag & Treatment Severe Hepatopancreat Dis, Wenzhou, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp 1, Dept Urol Surg, Wenzhou, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 1, Hepatobiliary Pancreat Tumor Bioengn Cross Int Joi, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Fuxue Rd, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	附属第一医院_肝胆外科; 附属第一医院
第一作者的第一单位	附属第一医院_肝胆外科
推荐引用方式 GB/T 7714	Zhu, Xuewen,Bao, Wenming,Xie, Xiaozai,et al. Liensinine inhibits progression of intrahepatic cholangiocarcinoma by regulating TGF-β1 /P-smad3 signaling through HIF-1a[J]. MOLECULAR CARCINOGENESIS,2024.
APA	Zhu, Xuewen., Bao, Wenming., Xie, Xiaozai., Chen, Bo., Li, Rizhao., ... & Li, Jiacheng. (2024). Liensinine inhibits progression of intrahepatic cholangiocarcinoma by regulating TGF-β1 /P-smad3 signaling through HIF-1a. MOLECULAR CARCINOGENESIS.
MLA	Zhu, Xuewen,et al."Liensinine inhibits progression of intrahepatic cholangiocarcinoma by regulating TGF-β1 /P-smad3 signaling through HIF-1a".MOLECULAR CARCINOGENESIS (2024).