Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study

doi:10.3389/fmicb.2023.1320279

科研成果详情

题名	Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study
作者	Pan, Tongtong 1; Su, Lihuang 2; Zhang, Yiying 3; Yi, Fangfang1; Chen, Yongping1
发表日期	2024-01-08
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article ; Journal Article
关键词	gut microbiota nonalcoholic fatty liver disease Mendelian randomization colonystimulating factor 2 receptor beta fucosyltransferase 2 17-beta-hydroxysteroid dehydrogenase 14 colonystimulating factor 2 receptor β
其他关键词	GENOME-WIDE ASSOCIATION ; OBESITY ; HEALTH
摘要	Introduction: Enteric dysbacteriosis is strongly associated with nonalcoholic fatty liver disease (NAFLD). However, the underlying causal relationship remains unknown. Thus, the present study aimed to investigate the relationship between gut microbiota and NAFLD using Mendelian randomization (MR) and analyze the target genes potentially regulated by specific microbiota. Methods: Bidirectional two-sample MR analysis was performed using inverse variance weighted (IVW) supplemented by MR-Egger, weighted median, simple mode, and weighted mode methods. Data were pooled from gut microbiota and NAFLD association studies. The least absolute shrinkage, selection operator regression, and the Support Vector Machine algorithm were used to identify genes regulated by these intestinal flora in NAFLD. The liver expression of these genes was verified in methionine choline-deficient (MCD) diet-fed mice. Results: IVW results confirmed a causal relationship between eight specific gut microbes and NAFLD. Notably, the order Actinomycetales, NB1n, the family Actinomycetaceae, Oxalobacteraceae and the genus Ruminococcaceae UCG005 were positively correlated, whereas Lactobacillaceae, the Christensenellaceae R7 group, and Intestinibacter were negatively correlated with NAFLD onset. In NAFLD, these eight bacteria regulated four genes: colony-stimulating factor 2 receptor beta, fucosyltransferase 2, 17-beta-hydroxysteroid dehydrogenase 14, and microtubule affinity regulatory kinase 3 (MAPK3). All genes, except MARK3, were differentially expressed in the liver tissues of MCD diet-fed mice. Discussion: The abundance of eight gut microbiota species and NAFLD progression displayed a causal relationship based on the expression of the four target genes. Our findings contributed to the advancement of intestinal microecology-based diagnostic technologies and targeted therapies for NAFLD.
资助项目	National Natural Science Foundation of China [82070593]; Natural Science Foundation of Zhejiang Province [LD21H030002]
出版者	FRONTIERS MEDIA SA
ISSN	1664-302X
EISSN	1664-302X
卷号	14
DOI	10.3389/fmicb.2023.1320279
页数	10
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001154984900001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	3826091
SCOPUSEID	2-s2.0-85182646686
通讯作者地址	[Chen, Yongping]Hepatology Diagnosis and Treatment Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,Wenzhou,China
Scopus学科分类	Microbiology;Microbiology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/207237
专题	附属第一医院阿尔伯塔学院
通讯作者	Chen, Yongping
作者单位	1.Hepatology Diagnosis and Treatment Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseases,Wenzhou,China; 2.The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Alberta Institute,Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Pan, Tongtong,Su, Lihuang,Zhang, Yiying,et al. Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study[J]. FRONTIERS IN MICROBIOLOGY,2024,14.
APA	Pan, Tongtong, Su, Lihuang, Zhang, Yiying, Yi, Fangfang, & Chen, Yongping. (2024). Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study. FRONTIERS IN MICROBIOLOGY, 14.
MLA	Pan, Tongtong,et al."Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study".FRONTIERS IN MICROBIOLOGY 14(2024).